- Open Access
Molecular characterization of extended-spectrum beta-lactamases (ESBLs) produced by clinical isolates of Acinetobacter baumannii in Saudi Arabia
© Alyamani et al. 2015
- Received: 21 May 2015
- Accepted: 7 August 2015
- Published: 20 August 2015
Acinetobacter baumannii is a common opportunistic pathogen that causes major nosocomial infections in hospitals. In this study, we hypothesized a high prevalence of A. baumanni ESBL (extended-spectrum beta-lactamase) among all collected isolates.
A. baumannii isolates (n = 107) from ICU (Intensive care unit) of local hospitals in Makkah were phenotypically and genotypically characterized. The identity and antibiotic susceptibility of A. baumannii strains were determined using the Vitek-2 system. The identified ESBL producers were further analyzed by PCR and sequencing followed by MLST typing. bla TEM , bla SHV , and the bla CTX-M-group genes 1, 2, 8, 9, and 25 were investigated. Furthermore, bla OXA51-like and bla OXA23-like genes were also examined in the carbapenem-resistant A. baumannii isolates.
Our data indicated a high prevalence of A. baumannii ESBL producers among the collected strains. Of the 107 A. baumannii isolates, 94 % were found to be resistant to cefepime and ceftazidime, and aztreonam using the Vitek 2 system. The genes detected encoded TEM, OXA-51-like and OXA-23-like enzymes, and CTX-M-group proteins 1, 2, 8, 9, and 25. MLST typing identified eight sequence type (ST) groups. The most dominant STs were ST195 and ST557 and all of them belong to worldwide clonal complex (CC) 2.
This study has shown that there is a high prevalence of antimicrobial resistance in A. baumannii. The diversity of STs may suggest that new ESBL strains are constantly emerging. The molecular diversity of the ESBL genes in A. baumannii may have contributed to the increased antimicrobial resistance among all isolates.
- Acinetobacter baumannii
- Saudi Arabia
Acinetobacter baumannii is an opportunistic and rapidly emerging pathogen. It is an important agent of nosocomial infections worldwide, such as urinary tract infections, septicemia, pneumonia, burns, meningitis, and wound infections in hospitals, due to its remarkable propensity to rapidly acquire resistance determinants to a wide range of antibacterial agents [1–4]. Many studies have documented high rates of multidrug-resistance (MDR) in A. baumannii [4–6]. The development of resistance to the third-generation cephalosporins was a major breakthrough in the fight against MDR strains. However, due to the frequent use of these agents, new plasmids encoding β-lactamase capable of hydrolyzing extended-spectrum cephalosporins were first reported in 1983 [7, 8]. These extended-spectrum β-lactamases (ESBLs) are mutant, plasmid-mediated, and produced by gram negative bacilli that mediate resistance to penicillin, cephalosporins, and monobactams . These ESBLs are commonly recognized in Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii and are found worldwide . The majority of ESBLs are members of either the TEM, SHV, or CTX-M (class A) families based on the Ambler molecular classification of β-lactamase genes [11, 12]. One of the major genes of ESBL family is the CTX-M, which is divided into five phylogenetic groups based on amino acid sequence identity: the CTX-M-1 group, the CTX-M-2 group, the CTX-M-8 group, the CTX-M-9 group, and the CTX-M-25 group. The presence and prevalence of these different groups are variable depending on the geographical locale [13, 14]. In Saudi Arabia, the high prevalence of ESBL A. baumannii was reported in several studies [15–17]. The PCR technology is widely used technique to screen for ESBL in modern hospitals. A specific multiplex PCR assay has been optimized to screen for multiple ESBL genes to facilitate and monitoring the spread and emergence of ESBL-producing bacteria . The epidemiologic characterization of A. baumannii by multilocus sequence typing (MLST) is a highly used method and has been applied successfully . With reports on the high prevalence of ESBL production in members of A. baumannii globally and a paucity of information specifically regarding the emergence of ESBL A. baumannii in major Saudi general hospitals in Makkah, this study reports the analysis of the antibiotic susceptibility profiles and molecular characterization of 107 A. baumannii ESBL producers isolated from ICU ward based on the phenotypic and genotypic approach. Understanding the molecular nature of the spread of A. baumannii in local hospitals is important, especially in hospitals that admit thousands of local and foreign people during their holy journey to Makkah. This work may enhance our understanding of the extent of the epidemiologic re-emergence of this bacterium. The genes that were investigated from A. baumannii isolates by PCR were bla TEM , bla SHV , and the bla CTX-M-group genes 1, 2, 8, 9, and 25. Furthermore, bla OXA51-like and bla OXA23-like enzymes were examined in carbapenem-resistant A. baumannii. This work may partially contribute to the global effort to map the molecular signature of A. baumannii.
A total of 107 bacterial isolates were collected from different ICU patients from clinical labs at local general hospitals in Makkah during 2 years from 2012 to 2014. Samples were subjected to a conventional microbiology analysis, phenotyping, and genotyping characterizations at the national center for biotechnology, KACST. The nature of the samples were blood, and skin wound infections predominantly.
Species identification and antimicrobials susceptibilities
Bacterial identities were confirmed using the Vitek 2 system (GN ID Card, bioMérieux, Craponne, France) and PCR. Antibiotic susceptibility testing was conducted according to the manufacturer’s recommendations (gram negative antimicrobial susceptibility testing (AST) cards, bioMérieux, Craponne, France). The extraction of genomic DNA was performed using QIAGEN kits (QIAamp DNA Mini Kit, cat# 69506, QIAGen, Valencia, CA, USA) according to the manufacturer’s recommendations and or the MagNA Pure LC DNA Isolation Kit III Bacteria, Fungi (Roche, Basel, Switzerland). The results of Vitek ESBL susceptibility test were reported according to the CLSI criteria. Quality-control bacterial strains (E. coli ATCC 35218 and Pseudomonas aeruginosa ATCC 27853) were used in all tests.
Detection of ESBL and carbapenem genes by PCR
Primers for the rapid characterization of A. baumannii by multiplex PCR
blaOXA-like enzymes of A. baumannii
5′-TAA TGC TTT GAT CGG CCT TG
Initial denaturation at 94 °C for 5 min, followed by 30 cycles of 94 °C for 25 s, 52 °C for 40 s and 72 °C for 50 s, and a final elongation at 72 °C for 6 min
5′-TGG ATT GCA CTT CAT CTT GG
5′-GAT CGG ATT GGA GAA CCA GA
5′-ATT TCT GAC CGC ATT TCC AT
5′-AAA AAT CAC TGC GCC AGT TC
Initial denaturation at 94 °C for 5 min, followed by 30 cycles of 94 °C for 25 s, 52 °C for 40 s and 72 °C for 50 s, and a final elongation at 72 °C for 6 min
5′-AGC TTA TTC ATC GCC ACG TT
5′- CGA CGC TAC CCC TGC TAT T
5′-CCA GCG TCA GAT TTT TCA GG
5′-CAA AGA GAG TGC AAC GGATG
5′-ATT GGA AAG CGT TCA TCA CC
5′-TCG CGT TAA GCG GAT GAT GC
5′-AAC CCA CGA TGT GGG TAG C
5′-GCA CGA TGA CAT TCG GG
5′-AAC CCA CGA TGT GGG TAG C
Initial denaturation at 94 °C for 10 min, followed by 30 cycles at 94 °C for 40 s, 60 °C for 40 s, and 72 °C for 1 min, and a final elongation step at 72 °C for 7 min
16S rRNA 8F
5′-GCG GAT CCG CGG CCG CTG CAG AGT TTG ATC CTG GCT CAG
Initial denaturation at 94 °C for 5 min, followed by 35 cycles at 94 °C for 60 s, 55 °C for 30 s, and 72 °C for 60 s, and a final elongation step at 72 °C for 7 min
16S rRNA 805R
5′-GCG GAT CCG CGG CCG CGG ACT ACC AGG GTA TCT AAT
Amplification and sequencing of the 16S rRNA gene
Amplification and sequencing of 16S rRNA were performed to confirm the identity of A. baumannii used in this study . In the PCR amplification, each reaction contained 25 μl of illustra PuReTaq Ready-To-Go PCR beads (GE Health Biosciences, USA). The reaction was set up as follows: 22 μl of nuclease-free water (Promega), 2 µl of 10 pmol of each forward and reverse primer (Eurofins MWG Operon, Germany) were used (Table 1) . Exactly 1 μl of 100 ng/µl DNA template was added to the beads. The amplification conditions are highlighted in Table 1. The amplification products were subjected to gel electrophoresis in 1 % agarose followed by ethidium bromide staining and were visualized under ultraviolet illumination using the Gel Doc EZ system (Bio-Rad, Hercules, CA, USA). Sense and anti-sense strands of PCR amplicons were purified and sequenced in an ABI 3130 Genetic Analyzer (Life Technologies, Carlsbad, CA, USA) using ABI BigDye terminator cycle sequencing ready reaction kit chemistry according to the manufacturer’s recommendations. Following sequencing, the data were identified using a basic local alignment search tool BLAST-n (http://www.ncbi.nlm.nih.gov/BLAST) or RDP database . The identification of A. baumannii using the 16S rRNA was unequivocal. Therefore, there was no need to use additional confirmatory targets such as rpoB and gyrB genes .
Multilocus sequence typing (MLST)
Primers used in PCR to amplify the seven housekeeping genes in A. baumannii isolates
Amplicon size (bp)
AAT TTA CAG TGG CAC ATT AGG TCC C
GCA GAG ATA CCA GCA GAG ATA CAC G
TGA AGG CGG CTT ATC TGA GT
GCT GGG TCT TTT TCC TGA CA
GCT ACT TTT ATG CAA CAG AGC C
GDH SEC F
ACC ACA TGC TTT GTT ATG
GTT GAG TTG GCG TAT GTT GTG C
GDH SEC R
GTT GGC GTA TGT TGT GC
CCT GAA TCT TCY GGT AAA AC
GTT TCT GGG CTG CCA AAC ATT AC
GGT GCT CAA CTT GTT CGT GA
CAC CGA AAC CAG GAG CTT TA
GAA ATT TCC GGA GCT CAC AA
TCA GGA GCA ATA CCC CAC TC
ACC CGT GAA GGT GAA ATC AG
TTC AGC TGG AGC TTT AGC AAT
Ethical approval and consent were not required for this project because no human nor animal subjects were used.
Antimicrobial susceptibility testing and screening for ESBL
Detection and ESBL genotyping of 107 Acinetobacter baumannii clinical isolates
Positive isolates of 107 isolates
Multilocus sequence typing analysis
In this study, we detected and characterized the phenotypic and genotypic nature of ESBL producers in A. baumannii, which were isolated from general hospitals in Makkah, Saudi Arabia. At least 107 A. baumannii isolates were characterized by the Vitek-2 system and PCR-sequencing followed by MLST typing. Our data indicated a high prevalence of A. baumannii ESBL producers among the collected isolates. A remarkable outcome of this study was the large number of antibiotic resistance genes found in these isolates. Ninety-four percent of A. baumannii isolates were found to have three major resistant determinants. We speculate that if more drug-resistant genes were screened, we would have found pan-resistant A. baumannii isolates.
CTX-M β-lactamases produced by A.baumannii strains is plasmid-mediated hence the wide spread and long time survival in hospitals. The CTX-M gene activity conferring resistance to cefotaxime and ceftazidime. We detected CTX-M group 1, 2, 8, 9, 25 in our current study (81 %). The high rate of prevalence of CTX-M resistance in gram negative bacteria may be influenced by mobile genetic elements around these genes which include transposon, insertion sequences (IS) and integrons . Consistent with our study, all gram negative CTX-M producing bacteria are often associated with other families of other β-lactamases resistance causing multi-drug resistance phenomena. The high prevalence rate around the world of CTX-M makes it a predominant drug resistant gene in gram negative bacteria .
We studied the dynamic spread of A.baumannii in our population by MLST. The discriminatory power of the MLST system is comparable to other techniques such as pulsed field gel electrophoresis (PFGE). Yet, MLST provides a quick and easy method to study the epidemiology of ESBL-producing bacteria and to monitor the international emergence of multidrug resistant bacteria. Consistent with other studies that used MLST in the epidemiologic characterization of clinically important bacterial pathogens such as A.baumannii, Streptococcus pneumoniae, Streptococcus pyogenes, Neisseria meningitidis, Campylobacter jejuni, Staphylococcus aureus, Enterococcus faecium, Haemophilus influenza, and Vibrio cholera, our study has detected different allelic diversity (STs) which belongs to clonal complex (CC)2 which is globally distributed in Europe, Asia, Africa, Australia, USA, South America [19, 27–29].
The drug of choice to treat nosocomial infection caused by A.baumannii is the carbapenems. However, there is an increasing rate of carbapenem-resistant A.baumannii around the world caused by OXA23-like enzume or OXA51-like enzyme acitivies . The first OXA23-like enzyme with carbapenem-activity to A. baumannii was isolated and characterized in Scotland in 1985. This drug-resistant determinant is encoded by the plasmid therefore it is transferable . This may explain the high prevalence of carbapenemase-producing A. baumannii in hospitals around the world. The other gene cluster in the OXA family is the bla OXA-51-like gene which is chromosomally encoded and naturally occurs in A. baumannii. The functional product of this gene delivers carbapenemase resistance to meropenem and imipenem; its role in carbapenem resistance may be influenced by the presence of ISAba1. PCR mapping studies have found that the absence of this sequence upstream of blaOXA-51-like gene may contribute to a minimal effect on carbapenem susceptibility [32–36].
A recent study in the Gulf Countries Council (GCC) , namely, Saudi Arabia, the United Arab Emirates, Oman, Qatar, Bahrain, and Kuwait, suggested a high prevalence of carbapenemase resistance in A. baumannii, Escherichia coli and Klebsiella pneumonia. A. baumannii (n = 117) was studied as clusters in seven different sequence types: ST195, ST208, ST229, ST436, ST450, ST452 and ST499. Three of these sequences were identified in our study, including ST195, ST499, and ST208, which may suggest the circulation of these three STs in GCC countries [17, 37]. The circulation of the STs within GCC may be due to the closeness of these countries to each other. Recent reports have been accumulating from Saudi Arabia due to the wide and rapid spread of carbapenem-resistant gram negative bacteria isolated from local hospitals specially during high season [38–40].
The high level of detection of ESBL and carbapenemase resistance among local isolates may suggest an increasing incidence rate of infection with ESBL-producing A. baumannii. Such high rates of ESBL-producing bacteria may impose a burden on routine clinical practice, especially for patients with chronic diseases and immunocompromised patients. Although national surveillance data are lacking, outbreaks of infection due to ESBL-producing A. baumannii have been reported by many hospitals within the Kingdom of Saudi Arabia. The true prevalence of ESBL producers is not known and is likely underestimated because of the difficulties encountered in their detection by most local hospitals. However, it is clear that ESBL-producing bacteria are distributed worldwide and their prevalence is increasing [2, 6, 41, 42]. Therefore, periodic screening of ESBL-producing A. baumannii during the high hospital visitation season is recommended in all local hospitals to establish national surveillance data archives of the level of spread of ESBL producers.
In this study, we randomly surveyed and characterized ESBL-producing A. baumannii from ICU of local hospitals in Makkah city, Saudi Arabia. Our data indicated a high prevalence of A. baumannii ESBL producers among the collected isolates. Based on MLST typing, we have evidence of eight STs groups in our isolates. The epidemiologic diversity of these isolates may suggest that new ESBL strains are constantly emerging. The molecular diversity of the ESBL genes in A. baumannii may have contributed to the increased antimicrobial resistance among all isolates. Therefore, periodic screening of ESBL-producing A. baumannii during the high hospital visitation season is recommended in all local hospitals.
EA and MK contributed to study design. RB, FB, BA, MA collected the samples and all contributed equally to the lab experiments. All authors contributed to data interpretation. EA and MK drafted and revised the manuscript. All authors read and approved the final manuscript.
This study was supported by internal grant from the National Center for Biotechnology at King Abdulaziz City for Science and Technology in Riyadh.
Compliance with ethical guidelines
Competing interests The authors declare that they have no competing interests.
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- Eliopoulos GM, Maragakis LL, Perl TM (2008) Acinetobacter baumannii: epidemiology, antimicrobial resistance, and treatment options. Clin Infect Dis 46(8):1254–1263View ArticleGoogle Scholar
- Antunes L, Visca P, Towner KJ (2014) Acinetobacter baumannii: evolution of a global pathogen. Pathog Dis 71(3):292–301View ArticlePubMedGoogle Scholar
- Srinivasan VB, Rajamohan G, Pancholi P, Stevenson K, Tadesse D, Patchanee P, Marcon M, Gebreyes WA (2009) Genetic relatedness and molecular characterization of multidrug resistant Acinetobacter baumannii isolated in central Ohio, USA. Ann Clin Microbiol Antimicrob 8(1):21PubMed CentralView ArticlePubMedGoogle Scholar
- Bergogne-Berezin E, Towner KJ (1996) Acinetobacter spp. as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9(2):148–165PubMed CentralPubMedGoogle Scholar
- Peleg AY, Seifert H, Paterson DL (2008) Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 21(3):538–582PubMed CentralView ArticlePubMedGoogle Scholar
- Perez F, Hujer AM, Hujer KM, Decker BK, Rather PN, Bonomo RA (2007) Global challenge of multidrug-resistant Acinetobacter baumannii. Antimicrob Agents Chemother 51(10):3471–3484PubMed CentralView ArticlePubMedGoogle Scholar
- Knothe H, Shah PDP, Krcmery V, Antal M, Mitsuhashi S (1983) Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens. Infection 11(6):315–317View ArticlePubMedGoogle Scholar
- Philippon A, Labia R, Jacoby G (1989) Extended-spectrum beta-lactamases. Antimicrob Agents Chemother 33(8):1131PubMed CentralView ArticlePubMedGoogle Scholar
- Rawat D, Nair D (2010) Extended-spectrum β-lactamases in Gram negative bacteria. J Glob Infect Dis 2(3):263PubMed CentralView ArticlePubMedGoogle Scholar
- Fluit AC, Visser MR, Schmitz FJ (2001) Molecular detection of antimicrobial resistance. Clin Microb Rev 14(4):836–871View ArticleGoogle Scholar
- Bush K, Jacoby GA (2010) Updated functional classification of β-lactamases. Antimicrob Agents Chemother 54(3):969–976PubMed CentralView ArticlePubMedGoogle Scholar
- Paterson DL, Bonomo RA (2005) Extended-spectrum β-lactamases: a clinical update. Clin Microbiol Rev 18(4):657–686PubMed CentralView ArticlePubMedGoogle Scholar
- Naas T, Oxacelay C, Nordmann P (2007) Identification of CTX-M-type extended-spectrum-β-lactamase genes using real-tme PCR and pyrosequencing. Antimicrob Agents Chemother 51(1):223–230PubMed CentralView ArticlePubMedGoogle Scholar
- Naas T, Cuzon G, Bogaerts P, Glupczynski Y, Nordmann P (2011) Evaluation of a DNA microarray (Check-MDR CT102) for rapid detection of TEM, SHV, and CTX-M extended-spectrum beta-lactamases and of KPC, OXA-48, VIM, IMP, and NDM-1 carbapenemases. J Clin Microbiol 49(4):1608–1613PubMed CentralView ArticlePubMedGoogle Scholar
- Al Johani SM, Akhter J, Balkhy H, El-Saed A, Younan M, Memish Z (2010) Prevalence of antimicrobial resistance among gram-negative isolates in an adult intensive care unit at a tertiary care center in Saudi Arabia. Ann Saudi Med 30(5):364PubMed CentralPubMedGoogle Scholar
- Al-Agamy MH, Shibl AM, Ali MS, Khubnani H, Radwan HH, Livermore DM (2014) Distribution of β-lactamases in carbapenem-non-susceptible Acinetobacter baumannii in Riyadh, Saudi Arabia. J Glob Antimicrob Resist 2(1):17–21View ArticleGoogle Scholar
- Zowawi HM, Sartor AL, Sidjabat HE, Balkhy HH, Walsh TR, Al Johani SM, AlJindan RY, Alfaresi M, Ibrahim E, Al-Jardani A et al (2015) Molecular epidemiology of carbapenem resistant Acinetobacter baumannii in the Gulf Cooperation Council States. Dominance of OXA-23-type producers. J Clin Microb 53:896–903View ArticleGoogle Scholar
- Dallenne C, Da Costa A, Decre D, Favier C, Arlet G (2010) Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J Antimicrob Chemother 65(3):490–495View ArticlePubMedGoogle Scholar
- Bartual SG, Seifert H, Hippler C, Luzon MA, Wisplinghoff H, Rodriguez-Valera F (2005) Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol 43(9):4382–4390PubMed CentralView ArticlePubMedGoogle Scholar
- Bosshard PP, Abels S, Altwegg M, Böttger EC, Zbinden R (2004) Comparison of conventional and molecular methods for identification of aerobic catalase-negative gram-positive cocci in the clinical laboratory. J Clin Microbiol 42(5):2065–2073PubMed CentralView ArticlePubMedGoogle Scholar
- Maidak BL, Olsen GJ, Larsen N, Overbeek R, McCaughey MJ, Woese CR (1996) The Ribosomal database project (RDP). Nucleic Acids Res 24(1):82–85PubMed CentralView ArticlePubMedGoogle Scholar
- Lee MJ, Jang SJ, Li XM, Park G, Kook J-K, Kim MJ, Chang Y-H, Shin JH, Kim SH, Kim D-M et al (2014) Comparison of rpoB gene sequencing, 16S rRNA gene sequencing, gyrB multiplex PCR, and the VITEK2 system for identification of Acinetobacter clinical isolates. Diagn Microbiol Infect Dis 78(1):29–34View ArticlePubMedGoogle Scholar
- Jolley K, Maiden M (2010) BIGSdb: scalable analysis of bacterial genome variation at the population level. BMC Bioinform 11(1):595View ArticleGoogle Scholar
- Larsen N, Olsen GJ, Maidak BL, McCaughey MJ, Overbeek R, Macke TJ, Marsh TL, Woese CR (1993) The ribosomal database project. Nucleic Acids Res 21(13):3021–3023PubMed CentralView ArticlePubMedGoogle Scholar
- Lahlaoui H, Ben Haj Khalifa A, Ben Moussa M (2014) Epidemiology of Enterobacteriaceae producing CTX-M type extended spectrum beta-lactamase (ESBL). Med Mal Infect 44(9):400–404View ArticlePubMedGoogle Scholar
- Canton R, Novais A, Valverde A, Machado E, Peixe L, Baquero F, Coque TM (2008) Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe. Clin Microbiol Infect 14(Suppl 1):144–153View ArticlePubMedGoogle Scholar
- Huber CA, Sartor AL, McOdimba F, Shah R, Shivachi P, Sidjabat HE, Revathi G, Paterson DL (2014) Outbreaks of multidrug-resistant Acinetobacter baumannii strains in a Kenyan teaching hospital. J Glob Antimicrob Resist 2(3):190–193View ArticleGoogle Scholar
- Jeannot K, Diancourt L, Vaux S, Thouverez M, Ribeiro A, Coignard B, Courvalin P, Brisse S (2014) Molecular epidemiology of carbapenem non-susceptible Acinetobacter baumannii in France. PLoS One 9(12):e115452PubMed CentralView ArticlePubMedGoogle Scholar
- Pournaras S, Gogou V, Giannouli M, Dimitroulia E, Dafopoulou K, Tsakris A, Zarrilli R (2014) Single-locus-sequence-based typing of blaOXA-51-like genes for rapid assignment of acinetobacter baumannii clinical isolates to international clonal lineages. J Clin Microbiol 52(5):1653–1657PubMed CentralView ArticlePubMedGoogle Scholar
- Evans BA, Hamouda A, Amyes SG (2013) The rise of carbapenem-resistant Acinetobacter baumannii. Curr Pharm Des 19(2):223–238View ArticlePubMedGoogle Scholar
- Paton R, Miles RS, Hood J, Amyes SG, Miles RS, Amyes SG (1993) ARI 1: beta-lactamase-mediated imipenem resistance in Acinetobacter baumannii. Int J Antimicrob Agents 2(2):81–87View ArticlePubMedGoogle Scholar
- Brown S, Young HK, Amyes SG (2005) Characterisation of OXA-51, a novel class D carbapenemase found in genetically unrelated clinical strains of Acinetobacter baumannii from Argentina. Clin Microbiol Infect Off Publ Eur Soc Clin Microbiol Infect Dis 11(1):15–23Google Scholar
- Coelho JM, Turton JF, Kaufmann ME, Glover J, Woodford N, Warner M, Palepou M-F, Pike R, Pitt TL, Patel BC (2006) Occurrence of carbapenem-resistant Acinetobacter baumannii clones at multiple hospitals in London and Southeast England. J Clin Microbiol 44(10):3623–3627PubMed CentralView ArticlePubMedGoogle Scholar
- Walther-Rasmussen J, Høiby N (2006) OXA-type carbapenemases. J Antimicrob Chemother 57(3):373–383View ArticlePubMedGoogle Scholar
- Turton JF, Ward ME, Woodford N, Kaufmann ME, Pike R, Livermore DM, Pitt TL (2006) The role of ISAba1 in expression of OXA carbapenemase genes in Acinetobacter baumannii. FEMS Microbiol Lett 258(1):72–77View ArticlePubMedGoogle Scholar
- Karah N, Sundsfjord A, Towner K, Samuelsen O (2012) Insights into the global molecular epidemiology of carbapenem non-susceptible clones of Acinetobacter baumannii. Drug Resist Updates Rev Comment Antimicrob Anticancer Chemother 15(4):237–247View ArticleGoogle Scholar
- Zowawi HM, Sartor AL, Balkhy HH, Walsh TR, Al Johani SM, AlJindan RY, Alfaresi M, Ibrahim E, Al-Jardani A, Al-Abri S et al (2014) Molecular characterization of carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in the countries of the Gulf cooperation council: dominance of OXA-48 and NDM producers. Antimicrob Agents Chemother 58(6):3085–3090PubMed CentralView ArticlePubMedGoogle Scholar
- Memish ZA, Assiri A, Almasri M, Roshdy H, Hathout H, Kaase M, Gatermann SG, Yezli S (2015) Molecular characterization of carbapenemase production among gram-negative bacteria in Saudi Arabia. Microb Drug Resist 21(3):307–314View ArticlePubMedGoogle Scholar
- Memish ZA (2002) Infection control in Saudi Arabia: meeting the challenge. Am J Infect Control 30(1):57–65View ArticlePubMedGoogle Scholar
- Aly M, Tayeb HT, Al Johani SM, Alyamani EJ, Aldughaishem F, Alabdulkarim I, Balkhy HH (2014) Genetic diversity of OXA-51-like genes among multidrug-resistant Acinetobacter baumannii in Riyadh, Saudi Arabia. Eur J Clin Microbiol Infect Dis Off Publ Eur Soc Clin Microbiol 33(7):1223–1228View ArticleGoogle Scholar
- Dijkshoorn L, Nemec A, Seifert H (2007) An increasing threat in hospitals: multidrug-resistant Acinetobacter baumannii. Nat Rev Microbiol 5(12):939–951View ArticlePubMedGoogle Scholar
- Diancourt L, Passet V, Nemec A, Dijkshoorn L, Brisse S (2010) The population structure of Acinetobacter baumannii: expanding multiresistant clones from an ancestral susceptible genetic pool. PLoS One 5(4):e10034PubMed CentralView ArticlePubMedGoogle Scholar