Articles

Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue

Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Ap...

Authors: Nguyen Thi Ngoc Phuong, Dao Huy Manh, Shyam Prakash Dumre, Shusaku Mizukami, Lan Nguyen Weiss, Nguyen Van Thuong, Tran Thi Ngoc Ha, Le Hong Phuc, Tran Van An, Thuan Minh Tieu, Mohamed Gomaa Kamel, Mostafa Ebraheem Morra, Vu Thi Que Huong, Nguyen Tien Huy and Kenji Hirayama

Migration crisis in Venezuela and its impact on HIV in other countries: the case of Colombia

Authors: Alfonso J. Rodríguez-Morales, D. Katterine Bonilla-Aldana, Miguel Morales, José A. Suárez and Ernesto Martínez-Buitrago

Characterization of the clonal profile of methicillin resistant Staphylococcus aureus isolated from patients with early post-operative orthopedic implant based infections

To analyze the molecular epidemiology and to compare between the major methicillin resistant Staphylococcus aureus biotypes for association with patient characteristics who had an implant for closed fracture and ...

Authors: Sonia Jain, Rukhsana Chowdhury, Mousumi Datta, Goutam Chowdhury and Asish Kumar Mukhopadhyay

Accurate identification and epidemiological characterization of Burkholderia cepacia complex: an update

Bacteria belonging to the Burkholderia cepacia complex (Bcc) are among the most important pathogens isolated from cystic fibrosis (CF) patients and in hospital acquired infections (HAI). Accurate identification o...

Authors: Naveen Kumar Devanga Ragupathi and Balaji Veeraraghavan

Antimicrobial stewardship program (ASP): an effective implementing technique for the therapy efficiency of meropenem and vancomycin antibiotics in Iranian pediatric patients

Antimicrobial stewardship program (ASP) is a distinguished method to improve the prescription and efficacy of antibiotics.

Authors: Aliakbar Rahbarimanesh, Sayed Yousef Mojtahedi, Payman Sadeghi, Maryam Ghodsi, Sara Kianfar, Leila Khedmat, Saeedreza Jamali Moghadam Siyahkali, Mohammad Kaji Yazdi and Anahita Izadi

The effect of malaria rapid diagnostic tests results on antimicrobial prescription practices of health care workers in Burkina Faso

Malaria rapid diagnostic tests (RDT) are widely used in endemic areas in order to comply with the recommendation that malaria treatment should only be given after the clinical diagnosis has been confirmed by R...

Authors: Massa dit Achille Bonko, Francois Kiemde, Marc Christian Tahita, Palpouguini Lompo, Athanase M. Some, Halidou Tinto, Michael Boele van Hensbroek, Petra F. Mens and Henk D. F. H. Schallig

Hypervirulent Klebsiella pneumoniae (hypermucoviscous and aerobactin positive) infection over 6 years in the elderly in China: antimicrobial resistance patterns, molecular epidemiology and risk factor

The definition of hypervirulent Klebsiella pneumoniae (hvKp), traditionally regarded as hypermucoviscosity, is controversial. However, data based on both phenotype (hypermucoviscous) and genetic (aerobactin) crit...

Authors: Chao Liu and Jun Guo

Streptococcus agalactiae from Ethiopian pregnant women; prevalence, associated factors and antimicrobial resistance: alarming for prophylaxis

Maternal Streptococcus agalactiae (Group B Streptococcus, GBS) colonization rates and its antibiotic resistance patterns provide important information useful in guiding prevention strategies. There is a paucity o...

Authors: Mucheye Gizachew, Moges Tiruneh, Feleke Moges, Mulat Adefris, Zemene Tigabu and Belay Tessema

Application of next-generation sequencing to detect variants of drug-resistant Mycobacterium tuberculosis: genotype–phenotype correlation

Drug resistance in Mycobacterium tuberculosis (MTB) is a major health issue worldwide. Recently, next-generation sequencing (NGS) technology has begun to be used to detect resistance genes of MTB. We aimed to ass...

Authors: Dae-Hyun Ko, Eun Jin Lee, Su-Kyung Lee, Han-Sung Kim, So Youn Shin, Jungwon Hyun, Jae-Seok Kim, Wonkeun Song and Hyun Soo Kim

Prevalence of Acinetobacter baumannii in Saudi Arabia: risk factors, antimicrobial resistance patterns and mechanisms of carbapenem resistance

Acinetobacter baumannii is an important opportunistic pathogen due to its capabilities for developing mechanisms of resistance to a wide range of antimicrobial agents including carbapenems. This review described ...

Authors: Mutasim E. Ibrahim

Cerebrospinal fluid penetration of very high-dose meropenem: a case report

Standard dosing of meropenem (2 g t.i.d.) produces CSF concentrations of only 1–2 mg/L which is inferior to the clinical breakpoint for most Gram-negative bacteria. There is therefore concern that dosing must ...

Authors: Thomas Kerz, Friederike D. von Loewenich, Jason Roberts, Axel Neulen and Florian Ringel

In vivo efficacy of tobramycin-loaded synthetic calcium phosphate beads in a rabbit model of staphylococcal osteomyelitis

Osteomyelitis is an acute or chronic inflammatory process of the bone following infection with pyogenic organisms like Staphylococcus aureus. Tobramycin (TOB) is a promising aminoglycoside antibiotic used to trea...

Authors: Godday Anebow Lulu, Arunkumar Karunanidhi, Loqman Mohamad Yusof, Yusuf Abba, Fazlin Mohd Fauzi and Fauziah Othman

Metagenomic next-generation sequencing as a diagnostic tool for toxoplasmic encephalitis

More than 100 different pathogens can cause encephalitis. Testing of all the neurological pathogens by conventional methods can be difficult. Metagenomic next-generation sequencing (NGS) could identify the inf...

Authors: Zhiliang Hu, Xing Weng, Chunhua Xu, Yang Lin, Cong Cheng, Hongxia Wei and Wei Chen

Detection of bacterial DNA from central venous catheter removed from patients by next generation sequencing: a preliminary clinical study

Catheter-related infection (CRI) is one of the serious challenges in clinical practice. This preliminary clinical study aimed to examine whether next-generation sequencing (NGS) targeting 16S rDNA, which was P...

Authors: Ken-ichi Okuda, Yutaka Yoshii, Satomi Yamada, Akio Chiba, Ippei Hironaka, Seiji Hori, Katsuhiko Yanaga and Yoshimitsu Mizunoe

Identification of pathogens in culture-negative infective endocarditis cases by metagenomic analysis

Pathogens identification is critical for the proper diagnosis and precise treatment of infective endocarditis (IE). Although blood and valve cultures are the gold standard for IE pathogens detection, many case...

Authors: Jun Cheng, Huan Hu, Yue Kang, Weizhi Chen, Wei Fang, Kaijuan Wang, Qian Zhang, Aisi Fu, Shuilian Zhou, Chen Cheng, Qingqing Cao, Feiyan Wang, Shela Lee and Zhou Zhou

Adherent/invasive Escherichia coli (AIEC) isolates from asymptomatic people: new E. coli ST131 O25:H4/H30-Rx virotypes

The widespread Escherichia coli clone ST131 implicated in multidrug-resistant infections has been recently reported, the majority belonging to O25:H4 serotype and classified into five main virotypes in accordance...

Authors: Edwin Barrios-Villa, Gerardo Cortés-Cortés, Patricia Lozano-Zaraín, Margarita María de la Paz Arenas-Hernández, Claudia Fabiola Martínez de la Peña, Ygnacio Martínez-Laguna, Carmen Torres and Rosa del Carmen Rocha-Gracia

Microbial virulence, molecular epidemiology and pathogenic factors of fluoroquinolone-resistant Haemophilus influenzae infections in Guangzhou, China

Fluoroquinolone-resistant Haemophilus influenzae (FRHI) has been reported worldwide but remain unclear in China.

Authors: Dingqiang Chen, Shuxian Wen, Donghua Feng, Ruirui Xu, Junyan Liu, Brian M. Peters, Danhong Su, Yongping Lin, Ling Yang, Zhenbo Xu and Mark E. Shirtliff

Culture- and PCR-based detection of BV associated microbiological profile of the removed IUDs and correlation with the time period of IUD in place and the presence of the symptoms of genital tract infection

The long-term use of intrauterine devices (IUDs) may lead to biofilm formation on the surface. The aim of this study was to perform the culture- and PCR-based detection of bacteria/fungi from the biofilm of th...

Authors: András Ádám, Zoltán Pál, Gabriella Terhes, Márta Szűcs, Israel David Gabay and Edit Urbán

Appropriateness of empiric antimicrobial therapy with imipenem/colistin in severe septic patients: observational cohort study

Empiric antimicrobial therapy (EAMT) using imipenem/colistin is commonly prescribed as a first line therapy in critically ill patients with severe sepsis. We aimed to assess the appropriateness of prescribing ...

Authors: Ahlem Trifi, Sami Abdellatif, Cyrine Abdennebi, Foued Daly, Rochdi Nasri, Yosr Touil and Salah Ben Lakhal

Clindamycin suppresses virulence expression in inducible clindamycin-resistant Staphylococcus aureus strains

Clindamycin is a protein synthesis inhibitory agent that has the ability to suppress the expression of virulence factors in Staphylococcus aureus. Recent guidelines recommend the use of clindamycin for the treatm...

Authors: Elisabeth Hodille, Cédric Badiou, Caroline Bouveyron, Michèle Bes, Anne Tristan, François Vandenesch, Gérard Lina and Oana Dumitrescu

Impact of the use of local fidaxomicin treatment algorithms for managing Clostridium difficile infection in hospitalized patients in southeastern United States

Clostridium difficile-associated diarrhea (CDAD) is a major public health threat that results in increased length of stay, hospital readmissions, deaths, and economic burden. CDAD treatment is often guided by sev...

Authors: Jonathan C. Cho, Sandy J. Estrada, Jamie J. Kisgen, Angelina Davis and Laura Puzniak

An update on gastrointestinal endoscopy-associated infections and their contributing factors

During clinical use, gastrointestinal endoscopes are grossly contaminated with patient’s native flora. These endoscopes undergo reprocessing to prevent infectious transmission upon future use. Endoscopy-associ...

Authors: Charles Eugenio McCafferty, Marra Jai Aghajani, David Abi-Hanna, Iain Bruce Gosbell and Slade Owen Jensen

Mycetoma: a clinical dilemma in resource limited settings

Mycetoma is a chronic mutilating disease of the skin and the underlying tissues caused by fungi or bacteria. Although recently included in the list of neglected tropical diseases by the World Health Organizati...

Authors: Pembi Emmanuel, Shyam Prakash Dumre, Stephen John, Juntra Karbwang and Kenji Hirayama

An evaluation of multidrug-resistant Escherichia coli isolates in urinary tract infections from Aguascalientes, Mexico: cross-sectional study

Uropathogenic Escherichia coli (UPEC) are one of the main bacteria causing urinary tract infections (UTIs). The rates of UPEC with high resistance towards antibiotics and multidrug-resistant bacteria have increas...

Authors: Flor Y. Ramírez-Castillo, Adriana C. Moreno-Flores, Francisco J. Avelar-González, Francisco Márquez-Díaz, Josée Harel and Alma L. Guerrero-Barrera

Overexpression of eis without a mutation in promoter region of amikacin- and kanamycin-resistant Mycobacterium tuberculosis clinical strain

Aminoglycosides such as amikacin and kanamycin are effective injectable second-line drugs for treatment of multidrug-resistant tuberculosis. Molecular mechanisms underlying aminoglycoside resistance are not we...

Authors: Angkanang Sowajassatakul, Therdsak Prammananan, Angkana Chaiprasert and Saranya Phunpruch

The Dengue virus in Nepal: gaps in diagnosis and surveillance

The introduction of the dengue virus (DENV) in Nepal is recent, first reports date back to 2004 from a Japanese traveller and limited information is available about DENV infection in the Nepali population. Wit...

Authors: Birendra Prasad Gupta, Andrea Haselbeck, Jerome H. Kim, Florian Marks and Tarun Saluja

Streptococcus pyogenes-purpura fulminans as an invasive form of group A streptococcal infection

Streptococcus pyogenes is an uncommon pathogen of purpura fulminans, and the pathogenesis of S. pyogenes-purpura fulminans remains unclear because of paucity of cases. We reported a pediatric case of S. pyogenes-...

Authors: Sayaka Okuzono, Masataka Ishimura, Shunsuke Kanno, Motoshi Sonoda, Noriyuki Kaku, Yoshitomo Motomura, Hisanori Nishio, Utako Oba, Masuo Hanada, Jun-ichi Fukushi, Michiyo Urata, Dongchon Kang, Hidetoshi Takada and Shouichi Ohga

Predictor of multidrug resistant tuberculosis in southwestern part of Ethiopia: a case control study

Curable disease tuberculosis is becoming incurable or difficult to treat due to drug resistance. Multi drug resistance tuberculosis is a major health problem for less developed countries. Development of drug r...

Authors: Dabesa Gobena, Gemechu Ameya, Kinfe Haile, Getaneh Abreha, Yoseph Worku and Tessema Debela

Vonoprazan-based triple therapy is non-inferior to susceptibility-guided proton pump inhibitor-based triple therapy for Helicobacter pylori eradication

All Helicobacter pylori-infected patients are recommended for eradication with an appropriate regimen in each geographic area. The choice of the therapy is somewhat dependent on the antimicrobial susceptibility. ...

Authors: Hiroki Tanabe, Keiichi Yoshino, Katsuyoshi Ando, Yoshiki Nomura, Katsuhisa Ohta, Kiichi Satoh, Eiichiro Ichiishi, Akiei Ishizuka, Takaaki Otake, Yutaka Kohgo, Mikihiro Fujiya and Toshikatsu Okumura

The draft genomes and investigation of serotype distribution, antimicrobial resistance of group B Streptococcus strains isolated from urine in Suzhou, China

The group B Streptococcus (GBS) is a human commensal bacterium, which is capable of causing several infectious diseases in infants, and people with chronic diseases. GBS has been the most common cause of infectio...

Authors: Yong Guo, Xiao Deng, Yuan Liang, Liang Zhang, Guo-Ping Zhao and Yan Zhou

Antimicrobial misuse in pediatric urinary tract infections: recurrences and renal scarring

In children, urinary tract infection (UTI) is one of a common bacterial infection. This study was conducted to detect the uropathogen, antimicrobial susceptibility, pathogen associated with recurrences and ren...

Authors: Jayaweera Arachchige Asela Sampath Jayaweera and Mohommed Reyes

Infectious disease burden and antibiotic prescribing in primary care in Israel

Antibiotics are frequently prescribed at many of the visits to primary care clinics, often for conditions for which they provide no benefit, including viral respiratory tract infections.

Authors: Marcelo Low, Ronit Almog, Ran D. Balicer, Nicky Liberman, Raul Raz, Avi Peretz and Orna Nitzan

Detection of urinary Chlamydia trachomatis, Mycoplasma genitalium and human papilloma virus in the first trimester of pregnancy by PCR method

Miscarriage and preterm delivery are the most important challenges of pregnancy. Different bacterial and viral infection may cause miscarriage and preterm delivery. Among bacterial factors, Mycoplasma genitalium ...

Authors: Monireh Rahimkhani, A. Mordadi and M. Gilanpour

Association of virulence gene expression with colistin-resistance in Acinetobacter baumannii: analysis of genotype, antimicrobial susceptibility, and biofilm formation

Acinetobacter baumannii causes difficult-to-treat nosocomial infections, which often lead to morbidity due to the development of antimicrobial drug resistance and expression of virulence genes. Data regarding the...

Authors: Abbas Bahador, Zahra Farshadzadeh, Reza Raoofian, Masoumeh Mokhtaran, Babak Pourakbari, Maryam Pourhajibagher and Farhad B. Hashemi

Antimicrobial susceptibility of Campylobacter jejuni and Campylobacter coli: comparison between Etest and a broth dilution method

Campylobacter is a leading cause of foodborne gasteroenteritis worldwide. Antimicrobial susceptibility testing for Campylobacter spp. is not routinely performed by most clinical laboratories. However, the emergen...

Authors: Maya Azrad, Linda Tkhawkho, Natalia Isakovich, Orna Nitzan and Avi Peretz

Rapid detection of carbapenemase activity of Enterobacteriaceae isolated from positive blood cultures by MALDI-TOF MS

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical...

Authors: Jiajia Yu, Jingxian Liu, Yuanrui Li, Jing Yu, Weinan Zhu, Ying Liu and Lisong Shen

Mycotic aneurysm caused by Edwardsiella tarda successfully treated with stenting and suppressive antibiotic therapy: a case report and systematic review

Mycotic aneurysm is an uncommon disease which could be fatal without appropriate treatment. Although standard therapy for mycotic aneurysms consists of resection of the infected aorta and in situ graft replace...

Authors: Kei Furui Ebisawa, Sho Nishimura, Shungo Yamamoto, Goh Ohji and Kentaro Iwata

Emergence of multidrug-resistant Providencia rettgeri isolates co-producing NDM-1 carbapenemase and PER-1 extended-spectrum β-lactamase causing a first outbreak in Korea

Nosocomial outbreak due to carbapenem-resistant Enterobacteriaceae has become serious challenge to patient treatment and infection control. We describe an outbreak due to a multidrug-resistant Providencia rettger...

Authors: Saeam Shin, Seok Hoon Jeong, Hyukmin Lee, Jun Sung Hong, Min-Jeong Park and Wonkeun Song

Raoultella planticola bacteremia-induced fatal septic shock following burn injury

Raoultella planticola, a Gram-negative, aerobic bacillus commonly isolated from soil and water, rarely causes invasive infections in humans. Septic shock from R. planticola after burn injury has not been previous...

Authors: Tetsuya Yumoto, Hiromichi Naito, Hiromi Ihoriya, Kohei Tsukahara, Tomoyuki Ota, Toshiyuki Watanabe and Atsunori Nakao

Nasal colonization with methicillin-resistant Staphylococcus aureus among elderly living in nursing homes in Brazil: risk factors and molecular epidemiology

Methicillin-resistant Staphylococcus aureus poses a threat to elderly living in nursing homes. Studies focusing on the epidemiology of colonization may help in the design of infection control strategies.

Authors: Monica da Silveira, Maria de Lourdes Ribeiro de Souza da Cunha, Camila Sena Martins de Souza, Adriana Aparecida Feltrin Correa and Carlos Magno Castelo Branco Fortaleza

Hepatitis E virus infection in Turkey: a systematic review

Hepatitis E virus (HEV), a non-enveloped single stranded RNA virus causes sporadic cases of hepatitis or outbreaks. The disease is generally self-limited although it may cause fulminant hepatitis in pregnant w...

Authors: Hakan Leblebicioglu and Resat Ozaras

Ginger (Zingiber officinale) phytochemicals—gingerenone-A and shogaol inhibit SaHPPK: molecular docking, molecular dynamics simulations and in vitro approaches

Antibiotic resistance is a defense mechanism, harbored by pathogens to survive under unfavorable conditions. Among several antibiotic resistant microbial consortium, Staphylococcus aureus is one of the most havoc...

Authors: Shailima Rampogu, Ayoung Baek, Rajesh Goud Gajula, Amir Zeb, Rohit S. Bavi, Raj Kumar, Yongseong Kim, Yong Jung Kwon and Keun Woo Lee

Comparison of nephrotoxicity of Colistin with Polymyxin B administered in currently recommended doses: a prospective study

The most important concern with polymyxins (Colistin and Polymyxin B) use is nephrotoxicity. There is no prospective data comparing nephrotoxicity of these two drugs, when administered in high doses and as per...

Authors: Ritesh Aggarwal and Arun Dewan

Detection of Neisseria meningitidis in a paediatric patient with septic arthritis using multiplexed diagnostic PCR targeting meningitis/encephalitis (ME)

Neisseria meningitidis is associated with meningitis and septicemia. Septic meningococcal arthritis is relatively uncommon and its diagnosis associated with clinical and microbiological challenges. Early recognit...

Authors: Donnchadh O’Sullivan, Barry Linnane, Amanda Mostyn, Nteimam Jonathan, Marie Lenihan, Nuala H. O’Connell and Colum P. Dunne

Factors associated to prevalence and treatment of carbapenem-resistant Enterobacteriaceae infections: a seven years retrospective study in three tertiary care hospitals

The increasing incidence of carbapenem-resistant Enterobacteriaceae (CRE), has resulted in a difficult problem in the current clinical anti-infective treatment. We performed a retrospective analysis of prevalence...

Authors: Feng Pang, Xiu-Qin Jia, Qi-Gang Zhao and Yi Zhang

Genetic contexts related to the diffusion of plasmid-mediated CTX-M-55 extended-spectrum beta-lactamase isolated from Enterobacteriaceae in China

CTX-M-55 extended-spectrum beta-lactamases are being rapidly disseminated and transmitted in clinical practices around the world. The genetic contexts of the transferable plasmid-mediated bla_CTX-M-55 gene in Ente...

Authors: Xiaoxin Hu, Jianjun Gou, Xiaobing Guo, Zaiqiu Cao, Yuan Li, Hongjian Jiao, Xiaohong He, Yihui Ren and Fuyun Tian

A case of Cardiobacterium valvarum endocarditis with cerebral hemorrhage after MVR, TVP and vegetation removal operation

Cardiobacterium is a fastidious Gram-negative bacillus, and is a rare human pathogen in clinical settings. Herein, we describe a case of Cardiobacterium valvarum (C. valvarum) endocarditis with a rare complicatio...

Authors: Lijia Ni, Xiaoying Xie, Nengyong Ouyang, Baiji Chen, Dongye Wang, Xiaoqiang Liu, Xiquan Wu, Jiajian Guo, Hongyu Li, Yandan Yao and Songyin Huang

Application of next-generation sequencing to characterize novel mutations in clarithromycin-susceptible Helicobacter pylori strains with A2143G of 23S rRNA gene

Clarithromycin (CLR) resistance has become a predominant factor for treatment failure of Helicobacter pylori eradication. Although the molecular mechanism of CLR resistance has been clearly understood in H. pylor...

Authors: Jiaoe Chen, Liping Ye, Liangmin Jin, Xuehua Xu, Peisong Xu, Xianjun Wang and Hongzhang Li

Prenatal and postnatal antibiotic exposure influences the gut microbiota of preterm infants in neonatal intensive care units

To explore the influences of prenatal antibiotic exposure, the intensity of prenatal and postnatal antibiotic exposure on gut microbiota of preterm infants and whether gut microbiota and drug resistant strains...

Authors: Zhi-Hui Zou, Dong Liu, Hong-Dong Li, Dan-Ping Zhu, Yu He, Ting Hou and Jia-Lin Yu

Quality control of malaria microscopy reveals misdiagnosed non-falciparum species and other microscopically detectable pathogens in Senegal

In developing countries, malaria diagnosis relies on microscopy and rapid diagnostic tests. In Senegal, national malaria control program (NMCP) regularly conducts supervisory visits in health services where ma...

Authors: Mamadou Alpha Diallo, Khadim Diongue, Mame Cheikh Seck, Mouhamadou Ndiaye, Ibrahima Diallo, Younouss Diedhiou, Tolla Ndiaye, Yaye Die Ndiaye, Aida Sadikh Badiane and Daouda Ndiaye