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TableĀ 2 Primers used in PCR to amplify the seven housekeeping genes in A. baumannii isolates

From: Molecular characterization of extended-spectrum beta-lactamases (ESBLs) produced by clinical isolates of Acinetobacter baumannii in Saudi Arabia

No. Locus Primer Sequences Amplicon size (bp) Usage
1 gltA Citrato F1 AAT TTA CAG TGG CAC ATT AGG TCC C 722 Amp/seq
Citrato R12 GCA GAG ATA CCA GCA GAG ATA CAC G Amp/seq
2 gyrB gyrB_F TGA AGG CGG CTT ATC TGA GT 594 Amp/seq
gyrB_R GCT GGG TCT TTT TCC TGA CA Amp/seq
3 gdhB GDHB 1F GCT ACT TTT ATG CAA CAG AGC C 774 Amp
GDH SEC F ACC ACA TGC TTT GTT ATG Seq
GDHB 775R GTT GAG TTG GCG TAT GTT GTG C Amp
GDH SEC R GTT GGC GTA TGT TGT GC Seq
4 recA RA1 CCT GAA TCT TCY GGT AAA AC 425 Amp/seq
RA2 GTT TCT GGG CTG CCA AAC ATT AC Amp/seq
5 cpn60 cpn60_F GGT GCT CAA CTT GTT CGT GA 640 Amp/seq
cpn60_R CAC CGA AAC CAG GAG CTT TA Amp/seq
6 gpi gpi_F GAA ATT TCC GGA GCT CAC AA 456 Amp/seq
gpi_R TCA GGA GCA ATA CCC CAC TC Amp/seq
7 rpoD rpoD-F ACC CGT GAA GGT GAA ATC AG 672 Amp/seq
rpoD-R TTC AGC TGG AGC TTT AGC AAT Amp/seq