Table 2 Primers used in PCR to amplify the seven housekeeping genes in A. baumannii isolates
No. | Locus | Primer | Sequences | Amplicon size (bp) | Usage |
|---|---|---|---|---|---|
1 | gltA | Citrato F1 | AAT TTA CAG TGG CAC ATT AGG TCC C | 722 | Amp/seq |
Citrato R12 | GCA GAG ATA CCA GCA GAG ATA CAC G | Amp/seq | |||
2 | gyrB | gyrB_F | TGA AGG CGG CTT ATC TGA GT | 594 | Amp/seq |
gyrB_R | GCT GGG TCT TTT TCC TGA CA | Amp/seq | |||
3 | gdhB | GDHB 1F | GCT ACT TTT ATG CAA CAG AGC C | 774 | Amp |
GDH SEC F | ACC ACA TGC TTT GTT ATG | Seq | |||
GDHB 775R | GTT GAG TTG GCG TAT GTT GTG C | Amp | |||
GDH SEC R | GTT GGC GTA TGT TGT GC | Seq | |||
4 | recA | RA1 | CCT GAA TCT TCY GGT AAA AC | 425 | Amp/seq |
RA2 | GTT TCT GGG CTG CCA AAC ATT AC | Amp/seq | |||
5 | cpn60 | cpn60_F | GGT GCT CAA CTT GTT CGT GA | 640 | Amp/seq |
cpn60_R | CAC CGA AAC CAG GAG CTT TA | Amp/seq | |||
6 | gpi | gpi_F | GAA ATT TCC GGA GCT CAC AA | 456 | Amp/seq |
gpi_R | TCA GGA GCA ATA CCC CAC TC | Amp/seq | |||
7 | rpoD | rpoD-F | ACC CGT GAA GGT GAA ATC AG | 672 | Amp/seq |
rpoD-R | TTC AGC TGG AGC TTT AGC AAT | Amp/seq |