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Table 1 Primers for the rapid characterization of A. baumannii by multiplex PCR

From: Molecular characterization of extended-spectrum beta-lactamases (ESBLs) produced by clinical isolates of Acinetobacter baumannii in Saudi Arabia

No. blaOXA-like enzymes of A. baumannii Amplification conditions
 1 blaOXA-51 F 5′-TAA TGC TTT GAT CGG CCT TG 353 bp Initial denaturation at 94 °C for 5 min, followed by 30 cycles of 94 °C for 25 s, 52 °C for 40 s and 72 °C for 50 s, and a final elongation at 72 °C for 6 min
 2 blaOXA-51R 5′-TGG ATT GCA CTT CAT CTT GG
 3 blaOXA-23-F 5′-GAT CGG ATT GGA GAA CCA GA 501 bp
 4 blaOXA-23-R 5′-ATT TCT GAC CGC ATT TCC AT
blaCTX-M genes
 7 Group 1-F 5′-AAA AAT CAC TGC GCC AGT TC 415 bp Initial denaturation at 94 °C for 5 min, followed by 30 cycles of 94 °C for 25 s, 52 °C for 40 s and 72 °C for 50 s, and a final elongation at 72 °C for 6 min
 8 Group 1-R 5′-AGC TTA TTC ATC GCC ACG TT
 9 Group 2-F 5′- CGA CGC TAC CCC TGC TAT T 552 bp
 10 Group 2-R 5′-CCA GCG TCA GAT TTT TCA GG
 11 Group 9-F 5′-CAA AGA GAG TGC AAC GGATG 205 bp
 12 Group 9-R 5′-ATT GGA AAG CGT TCA TCA CC
 13 Group 8F 5′-TCG CGT TAA GCG GAT GAT GC 666 bp
 14 Group 8R 5′-AAC CCA CGA TGT GGG TAG C
 15 Group 25F 5′-GCA CGA TGA CAT TCG GG 327 bp
 16 Group 25R 5′-AAC CCA CGA TGT GGG TAG C
 1 TEM-F 5′-CATTTCCGTGTCGCCCTTATTC 800 bp Initial denaturation at 94 °C for 10 min, followed by 30 cycles at 94 °C for 40 s, 60 °C for 40 s, and 72 °C for 1 min, and a final elongation step at 72 °C for 7 min
 2 TEM-R 5′-CGTTCATCCATAGTTGCCTGAC
 3 SHV-F 5′-AGCCGCTTGAGCAAATTAAAC 713 bp
 4 SHV-R 5′-ATCCCGCAGATAAATCACCAC
 1 16S rRNA 8F 5′-GCG GAT CCG CGG CCG CTG CAG AGT TTG ATC CTG GCT CAG 797 bp Initial denaturation at 94 °C for 5 min, followed by 35 cycles at 94 °C for 60 s, 55 °C for 30 s, and 72 °C for 60 s, and a final elongation step at 72 °C for 7 min
 2 16S rRNA 805R 5′-GCG GAT CCG CGG CCG CGG ACT ACC AGG GTA TCT AAT