Table 1 Primers for the rapid characterization of A. baumannii by multiplex PCR

 1

blaOXA-51 F

5′-TAA TGC TTT GAT CGG CCT TG

353 bp

Initial denaturation at 94 °C for 5 min, followed by 30 cycles of 94 °C for 25 s, 52 °C for 40 s and 72 °C for 50 s, and a final elongation at 72 °C for 6 min

 2

blaOXA-51R

5′-TGG ATT GCA CTT CAT CTT GG

 3

blaOXA-23-F

5′-GAT CGG ATT GGA GAA CCA GA

501 bp

 4

blaOXA-23-R

5′-ATT TCT GAC CGC ATT TCC AT

blaCTX-M genes

 7

Group 1-F

5′-AAA AAT CAC TGC GCC AGT TC

415 bp

Initial denaturation at 94 °C for 5 min, followed by 30 cycles of 94 °C for 25 s, 52 °C for 40 s and 72 °C for 50 s, and a final elongation at 72 °C for 6 min

 8

Group 1-R

5′-AGC TTA TTC ATC GCC ACG TT

 9

Group 2-F

5′- CGA CGC TAC CCC TGC TAT T

552 bp

 10

Group 2-R

5′-CCA GCG TCA GAT TTT TCA GG

 11

Group 9-F

5′-CAA AGA GAG TGC AAC GGATG

205 bp

 12

Group 9-R

5′-ATT GGA AAG CGT TCA TCA CC

 13

Group 8F

5′-TCG CGT TAA GCG GAT GAT GC

666 bp

 14

Group 8R

5′-AAC CCA CGA TGT GGG TAG C

 15

Group 25F

5′-GCA CGA TGA CAT TCG GG

327 bp

 16

Group 25R

5′-AAC CCA CGA TGT GGG TAG C

 1

TEM-F

5′-CATTTCCGTGTCGCCCTTATTC

800 bp

Initial denaturation at 94 °C for 10 min, followed by 30 cycles at 94 °C for 40 s, 60 °C for 40 s, and 72 °C for 1 min, and a final elongation step at 72 °C for 7 min

 2

TEM-R

5′-CGTTCATCCATAGTTGCCTGAC

 3

SHV-F

5′-AGCCGCTTGAGCAAATTAAAC

713 bp

 4

SHV-R

5′-ATCCCGCAGATAAATCACCAC

 1

16S rRNA 8F

5′-GCG GAT CCG CGG CCG CTG CAG AGT TTG ATC CTG GCT CAG

797 bp

Initial denaturation at 94 °C for 5 min, followed by 35 cycles at 94 °C for 60 s, 55 °C for 30 s, and 72 °C for 60 s, and a final elongation step at 72 °C for 7 min

 2

16S rRNA 805R

5′-GCG GAT CCG CGG CCG CGG ACT ACC AGG GTA TCT AAT