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Trends in antibacterial resistance among Streptococcus pneumoniae isolated in the USA: update from PROTEKT US Years 1–4
Annals of Clinical Microbiology and Antimicrobials volume 7, Article number: 1 (2008)
The increasing prevalence of resistance to established antibiotics among key bacterial respiratory tract pathogens, such as Streptococcus pneumoniae, is a major healthcare problem in the USA. The PROTEKT US study is a longitudinal surveillance study designed to monitor the susceptibility of key respiratory tract pathogens in the USA to a range of commonly used antimicrobials. Here, we assess the geographic and temporal trends in antibacterial resistance of S. pneumoniae isolates from patients with community-acquired respiratory tract infections collected between Year 1 (2000–2001) and Year 4 (2003–2004) of PROTEKT US.
Antibacterial minimum inhibitory concentrations were determined centrally using the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method; susceptibility was defined according to CLSI interpretive criteria. Macrolide resistance genotypes were determined by polymerase chain reaction.
A total of 39,495 S. pneumoniae isolates were collected during 2000–2004. The percentage of isolates resistant to erythromycin, penicillin, levofloxacin, and telithromycin were 29.3%, 21.2%, 0.9%, and 0.02%, respectively, over the 4 years, with marked regional variability. The proportion of isolates exhibiting multidrug resistance (includes isolates known as penicillin-resistant S. pneumoniae and isolates resistant to ≥ 2 of the following antibiotics: penicillin; second-generation cephalosporins, e.g. cefuroxime; macrolides; tetracyclines; and trimethoprim-sulfamethoxazole) remained stable at ~30% over the study period. Overall mef(A) was the most common macrolide resistance mechanism. The proportion of mef(A) isolates decreased from 68.8% to 62.3% between Year 1 and Year 4, while the percentage of isolates carrying both erm(B) and mef(A) increased from 9.7% to 18.4%. Over 99% of the erm(B)+mef(A)-positive isolates collected over Years 1–4 exhibited multidrug resistance. Higher than previously reported levels of macrolide resistance were found for mef(A)-positive isolates.
Over the first 4 years of PROTEKT US, penicillin and erythromycin resistance among pneumococcal isolates has remained high. Although macrolide resistance rates have stabilized, the prevalence of clonal isolates, with a combined erm(B) and mef(A) genotype together with high-level macrolide and multidrug resistance, is increasing, and their spread may have serious health implications. Telithromycin and levofloxacin both showed potent in vitro activity against S. pneumoniae isolates irrespective of macrolide resistance genotype.
Streptococcus pneumoniae is a common causative pathogen in community-acquired respiratory tract infections (RTIs), including acute otitis media , acute bacterial exacerbations of chronic bronchitis , acute bacterial sinusitis , and community-acquired pneumonia . It is also a major cause of bacteremia .
During the last decade, antimicrobial resistance has increased both in the USA [6–13] and worldwide [14–17]. In particular, resistance to penicillin and the macrolides has spread rapidly among isolates of S. pneumoniae . Surveillance data have shown that, among S. pneumoniae isolates obtained from pediatric patients, the proportion exhibiting nonsusceptibility to penicillin increased each year after 1994, reaching 45% in 2000 . There has also been a marked shift to high-level resistance to penicillin and cephalosporins among isolates of S. pneumoniae. Furthermore, several published studies and case reports (reviewed by Rzeszutek and colleagues ) have suggested a link between pneumococcal macrolide resistance and treatment failure (resulting in hospitalization) in patients with community-acquired RTIs.
Macrolide resistance among S. pneumoniae is mediated by two major mechanisms: methylation of ribosomal macrolide target sites, encoded by the erm(B) gene, and drug efflux, encoded by the mef(A) gene . While erm(B)-mediated resistance predominates across much of the world, the dominant genotype in the USA is mef(A) . S. pneumoniae isolates with both erm(B) and mef(A) genes have also been documented in the USA [21, 22], and are typically multidrug resistant and clonal in nature [11, 23]. These findings have raised concerns over the continued clinical utility of antibacterial agents, such as the β-lactams and macrolides, for the empiric treatment of many community-acquired RTIs.
PROTEKT US (P rospective R esistant O rganism T racking and E pidemiology for the K etolide T elithromycin in the US) – a longitudinal surveillance study – was initiated in 2000 to monitor resistance trends in S. pneumoniae and other common RTI pathogens in the USA . A major aim of the program is to evaluate the activity of telithromycin, the first in a new class of antibacterial agents, and to compare its activity to that of other commonly used antibacterials. Data from the PROTEKT US study for 2000–2001 and 2001–2002 (Years 1 and 2) indicated a national prevalence of pneumococcal macrolide resistance of 31%, with rates approaching 40% in some southern regions of the country [10, 24]. Data from 2000–2003 (Years 1–3) of the PROTEKT US study  demonstrated that the proportion of S. pneumoniae isolates exhibiting multidrug resistance has stabilized (31%). However, geographic variations remain and there is an increasing prevalence of isolates with both erm(B) and mef(A) genes, which is associated with high-level macrolide and multidrug resistance. The potential spread of macrolide and multidrug resistance is of serious concern and requires further monitoring. Telithromycin, however, continues to display potent in vitro activity against S. pneumoniae, including against isolates with the combined erm(B)+mef(A) genotype.
This paper reports on the phenotypic susceptibility and the distribution of macrolide resistance genotypes for S. pneumoniae isolates collected in Year 4 (2003–2004) of the PROTEKT US study; in addition, it provides an update on temporal and geographic trends in resistance patterns over the 4 years of the study.
The numbers of centers across the USA that contributed samples were: 207 in Year 1 (2000–2001), 241 in Year 2 (2001–2002), 247 in Year 3 (2002–2003), and 183 in Year 4 (2003–2004). For the purposes of geographic analysis, centers were assigned to one of six regions: Northwest (Alaska, Idaho, Montana, Oregon, Washington, Wyoming), Northeast (Connecticut, Delaware, Indiana, Maryland, Massachusetts, Michigan, New Jersey, New York, Ohio, Pennsylvania, Vermont, Washington, DC), North-central (Illinois, Iowa, Kansas, Minnesota, Missouri, Nebraska, North Dakota, South Dakota, Wisconsin), Southwest (Arizona, California, Colorado, Nevada, New Mexico, Utah), Southeast (Florida, Georgia, Kentucky, North Carolina, Puerto Rico, South Carolina, Virginia, West Virginia), and South-central (Alabama, Arkansas, Louisiana, Oklahoma, Tennessee, Texas).
Pathogenic respiratory tract isolates of S. pneumoniae were obtained from pediatric and adult outpatients with community-acquired RTIs (bacterial sinusitis, acute otitis media, pharyngitis, community-acquired pneumonia, acute bacterial exacerbations of chronic bronchitis, and acute exacerbations of chronic obstructive pulmonary disease). S. pneumoniae isolates cultured from material collected from hospitalized patients within 48 hours of admission were also included. Sources of isolates included blood, sputum, bronchoalveolar lavage, middle-ear fluid (sampled by tympanocentesis), nasopharyngeal swabs or aspirates, and sinus aspirates. Patients with cystic fibrosis and those with nosocomial RTIs were excluded from the study, as were strains originating from existing banked collections and duplicate strains. The following demographic data were collected: age and sex of patient, infection type, culture source, in-/outpatient status, specimen accession number, and date of sample collection. Details of the methods for isolate storage, transportation, and identification have been reported previously .
Antibacterial susceptibility testing
Antibacterial minimum inhibitory concentrations (MICs) were determined using the Clinical and Laboratory Standards Institute (CLSI) broth microdilution method  at a central laboratory (CMI, Wilsonville, OR, USA). CLSI MIC interpretive criteria were used to determine susceptibility . Susceptibility of S. pneumoniae isolates to telithromycin was determined using breakpoints approved by both the CLSI and the US Food and Drug Administration (susceptible ≤ 1 μg/mL; intermediate 2 μg/mL; resistant ≥ 4 μg/mL) [26, 27]. Multidrug-resistant S. pneumoniae were defined as isolates resistant to ≥ 2 of the following antibiotics: penicillin; second-generation cephalosporins, e.g. cefuroxime; macrolides; tetracyclines; and trimethoprim-sulfamethoxazole.
Erythromycin-resistant (MIC ≥ 1 μg/mL) pneumococcal isolates were analyzed for the presence of erm(B), erm(A) subclass erm(TR), and mef(A) macrolide resistance genes. Isolates in Year 1 were analyzed using a multiplex rapid-cycle polymerase chain reaction (PCR) with microwell-format probe hybridization, as described previously ; a Taqman®-based PCR assay was used in Years 2–4 .
In total, 39,495 S. pneumoniae isolates were collected in the PROTEKT US study from 2000 to 2004 (Year 1: 10,103; Year 2: 10,012; Year 3: 10,886; and Year 4: 8,494). Patient demographics and the culture source of S. pneumoniae isolates were similar in each year of the study (Table 1). Overall for Years 1–4 combined, most isolates of S. pneumoniae were collected from patients aged 15–64 years (42%) and approximately 50% of patients were hospitalized. The most common source of isolates was sputum (40.2%), followed by blood (28.7%) and bronchoalveolar lavage (10.6%).
Antibacterial resistance patterns
High-level penicillin resistance (MIC ≥ 2 μg/mL) decreased during Years 1–4 (Year 1, 26.3%; Year 4, 16.5%) (Table 2) and was associated with a concomitant rise in intermediate-level resistance to this antimicrobial (MIC 0.12–1 μg/mL) over the study period (Year 1, 12.5%; Year 4, 20.0%). The incidence of erythromycin resistance was similar across all study years (29.3% overall). In general, resistance rates for other antimicrobials were also stable, with the exception of trimethoprim-sulfamethoxazole, which decreased from 33.9% in Year 1 to 24.1% in Year 4. The prevalence of resistance to telithromycin and levofloxacin was low; in each year, > 99% and > 98% of S. pneumoniae isolates were susceptible to telithromycin and levofloxacin, respectively.
Combined data for Years 1–4 confirm that geographic variations in the prevalence of pneumococcal resistance to either penicillin or erythromycin exist across the USA (Figure 1). For Year 4, resistance to penicillin was highest in the South-central (n = 1,333), Southeast (n = 1,025), and North-central (n = 1,999) regions (19.1% of isolates for each region) and lowest in the Southwest (n = 927; 10.0%). Penicillin resistance decreased in all regions between Years 1 and 4. The greatest reductions in penicillin resistance were observed in the Southeast (Year 1, 36.4%; Year 4, 19.1%), followed by the Southwest (Year 1, 27.0%; Year 4, 10.0%) and South-central (Year 1, 32.5%; Year 4, 19.1%) regions. In contrast, intermediate-level penicillin resistance increased in most regions (except in the Southwest region). In all regions, resistance rates for erythromycin were higher than those for penicillin. In Year 4, resistance to erythromycin was highest in the South-central region (39.4%) and lowest in the Southwest region (17.0%). Erythromycin resistance rates decreased from Year 1 to Year 4 in the Southeast (Year 1, 40.2%; Year 4, 32.6%) and Southwest (Year 1, 29.3%; Year 4, 17.0%) regions, and remained stable or increased slightly in all other regions.
The prevalence of S. pneumoniae isolates that demonstrated resistance to both penicillin and erythromycin decreased over the study period (Year 1, 20.4%; Year 4, 13.3%) (Table 3). Decreases in the proportion of isolates resistant to both drugs were observed between Years 3 and 4 in all regions, but were most apparent in the South-central (Year 3, 21.9%; Year 4, 15.3%) and Southeast (Year 3, 19.6%; Year 4, 15.3%) regions.
The prevalence of multidrug resistance among S. pneumoniae remained relatively stable over the study period, particularly for Years 2–4, at approximately 30% of isolates (Table 4). Some decreases in the proportions of multidrug-resistant isolates were observed in southern regions (Year 1/Year 4: Southeast, 44.6%/31.4%; Southwest, 35.7%/17.4%). In Year 3, the prevalence of multidrug resistance in the Southwest was 22.4%; this decreased to 17.4% by Year 4.
The temporal trends in the prevalence of resistance to between 1 and 6 antibacterial agents are shown in Figure 2. During Years 1–4, there was no increasing trend towards resistance of isolates to a greater number of antibacterial agents. The prevalence of isolates resistant to 3, 4, or 5 classes of antibacterial, however, was high and approximately 8% of isolates demonstrated resistance to 5 classes of antibiotic.
Data from Years 1–4 show that mef(A)-encoded resistance was consistently the most commonly expressed genotype (65.7%) (Figure 3). However, across the 4-year study period, the proportion of macrolide-resistant isolates carrying both the mef(A) and erm(B) genes increased dramatically. In Year 1, 9.7% of macrolide-resistant isolates possessed both the mef(A) and erm(B) genes; this proportion increased to 12.0% in Year 2, rising to 16.4% in Year 3 and 18.4% in Year 4. Over the same period, isolates exhibiting mef(A) alone decreased and those exhibiting erm(B) remained stable. Overall, for Years 1–4 > 99 % (1,605/1,615 of the erm(B)+mef(A) isolates) were multidrug resistant (455/456 [99.8%] in Year 4). Analysis of the in vitro activity of a range of antibacterials against mef(A)-positive isolates found that all demonstrated higher than previously reported levels of resistance to erythromycin (MIC90 16 μg/mL; MIC mode 16 μg/mL). MIC90 values against these strains were also high for azithromycin (≥ 16 μg/mL; MIC mode 8 μg/mL) and clarithromycin (≥ 8 μg/mL; MIC mode μg/mL) (Table 5). By contrast, telithromycin retained strong activity against mef(A)-positive strains, with MIC90 values of 0.5 μg/mL (MIC mode 0.12 μg/mL).
Discussion and Conclusion
As part of the PROTEKT US study we have analyzed a large dataset for S. pneumoniae isolates sampled from across the USA. This has resulted in the generation of valuable information regarding temporal and geographic changes in antibacterial resistance patterns over the 4-year period from 2000 to 2004.
The latest data collected between 2003 and 2004 (Year 4) confirm the results of previous reports indicating that the prevalence of pneumococcal penicillin resistance across the USA appears to be stable or is decreasing, whilst intermediate penicillin resistance is increasing slightly [13, 30]. There are a number of possible reasons why pneumococcal resistance to penicillin and some other antibacterials (e.g., trimethoprim-sulfamethoxazole) may have stabilized or begun to decrease in the USA . First, recent local  and national  campaigns to promote appropriate antibacterial prescribing may have exerted a downward pressure on resistance rates as this factor is one of the most important drivers of resistance in community-acquired infections . Another factor may be the introduction in 2000 of the pneumococcal conjugate vaccine (PCV7) for routine immunization of infants; resistance rates have traditionally been highest among the pediatric population and the use of PCV7 has been shown to decrease pneumococcal resistance not only among children but also in the population as a whole, via a herding effect . A third factor may be the introduction of fluoroquinolones as a treatment for respiratory tract infections in adults. As the use of these agents has increased, it is possible that use of other more traditional agents may have declined, thus reducing associated rates of resistance.
Although the overall levels of in vitro penicillin-nonsusceptibility in S. pneumoniae may be a cause for concern, the clinical importance of this phenomenon in the management of pneumococcal pneumonia has been questioned [35–37]. There is no evidence of widespread clinical failures among respiratory infections caused by S. pneumoniae strains classified as penicillin-resistant in vitro. Moreover, in respiratory infections documented as being due to resistant pneumococci, the infecting S. pneumoniae strain generally exhibits low-level in vitro resistance (penicillin MIC 1–2 μg/mL); in these cases, the infection can usually be successfully treated using high doses of β-lactam antibiotics . Nevertheless, careful monitoring of penicillin resistance rates should continue, especially since reports of S. pneumoniae strains with high-level penicillin resistance (MIC ≥ 8 μg/mL) have appeared recently [39, 40]. Furthermore, there is clear evidence that infections of the central nervous system caused by penicillin-resistant S. pneumoniae strains can be associated with the failure of β-lactam therapy [38, 41]. Consideration of the prevalent rates of penicillin resistance among S. pneumoniae is therefore important in the management of such infections.
In common with recent reports from the USA and other countries, macrolide resistance over the 4 years of PROTEKT US exceeded penicillin resistance in all US regions [13, 16, 17, 30, 42]. The macrolide resistance rate reported here for Year 4 was similar to those found for Years 1–3 (approximately 30%), suggesting that levels may have plateaued [12, 13].
In this study, the proportion of isolates in Year 4 exhibiting resistance to both penicillin and erythromycin decreased compared with previous years; this downward trend was also observed over Years 1–3 of the PROTEKT US study . As noted in other recent surveillance studies [7, 10–13, 43], data for Years 1–4 of PROTEKT US showed considerable regional variation in the rates of resistance to penicillin and erythromycin across the USA.
Macrolide resistance mediated by erm(B) has typically been associated with high-level resistance (MIC90 values of ≥ 64 μg/mL), while mef(A)-mediated resistance has historically been characterized by lower-level resistance (MIC90 values of 4–8 μg/mL) [15, 44]. The predominant mechanism of pneumococcal macrolide resistance in the USA is mediated by mef(A) . However, the latest PROTEKT US data presented here confirm that the prevalence of the mef (A) genotype is decreasing and that clones expressing both erm(B) and mef(A) genes are increasing in prevalence. Of additional importance, the mef(A)-positive isolates were found to exhibit levels of macrolide resistance that were higher (MIC mode 16 μg/mL) than those reported in previous surveillance studies (4–8 μg/mL) [15, 43]. This may impact on the ability of the macrolides to eradicate such strains from the sites of infection in patients with community-acquired RTIs.
Molecular epidemiology studies undertaken as part of PROTEKT US have shown that, of the erm(B)+mef(A) isolates analyzed, > 90 % are clonally related to the multidrug-resistant international Taiwan19F-14 clonal complex 271 . Since these strains show high-level macrolide and multidrug resistance, their spread across the USA represents a serious public health threat.
The introduction of the 7-valent pneumococcal vaccine (PCV7) in 2000 was intended to reduce the incidence of pneumococcal disease in children. Recent evidence suggests that this reduction has indeed occurred [19, 45], with decreases of 58% in 2001 and 66% in 2002 in the number of invasive pneumococcal infections in children. However, the vaccine does not provide coverage against all S. pneumoniae serotypes. Most dual erm(B)+mef(A) isolates have been characterized as either serotype 19A or 19F and, although serotype 19F is represented in the PCV7 vaccine, 19A is not. As a result, incidence of the nonvaccine serotype 19A multidrug-resistant clone is proportionally higher in the pediatric population than in the past. According to recent surveillance data covering pneumococcal isolates collected in the USA, the prevalence of vaccine serotype 19F has decreased since introduction of PCV7, while that of nonvaccine serotype 19A has concomitantly increased [34, 46]. Among erm(B)+mef(A) isolates collected for the PROTEKT US study in 2000–2001, serotype 19F was predominant over 19A (87% vs 8%); however, by 2003–2004, these two serotypes were of roughly equal prevalence (52% [19F] vs 46% [19A]) among erm(B)+mef(A) isolates .
Since the introduction of newer macrolide antibiotics, there has been a steady increase in macrolide resistance from year to year among pneumococci, which has been correlated with their consumption . Macrolide antibacterials are commonly used for the empiric treatment of community-acquired RTIs; therefore, pneumococcal macrolide resistance is of increasing concern in the clinical setting . In recent years, a number of reports have been published linking occurrences of macrolide treatment failure (often resulting in hospitalization with breakthrough bacteremia) to infection by macrolide-resistant strains of S. pneumoniae in patients with community-acquired RTIs. Clinical failures in patients treated with azithromycin and clarithromycin have been documented [48–51], and the number of reports appears to be increasing. It is notable that clinical failures have been reported in patients infected with pneumococcal strains expressing mef(A)-encoded macrolide resistance as well as in those with erm(B)-mediated resistance [52, 53]. Expression of both erm(B) and mef(A) among S. pneumoniae isolates is strongly associated with the emergence of multidrug resistance. Almost all of the isolates (99.8%) expressing this dual mechanism of macrolide resistance in Year 4 of the study exhibited such resistance. Multidrug resistance has also been linked to an increased risk of clinical failure .
Telithromycin represents the first in a new class of antimicrobials – the ketolides. Telithromycin demonstrated potent in vitro activity against S. pneumoniae isolates, including erm(B)+mef(A) macrolide-resistant strains. The in vitro susceptibility of S. pneumoniae to telithromycin was very high in each of the study years, irrespective of the macrolide resistance mechanism. Overall, > 99% of macrolide-resistant S. pneumoniae isolates were susceptible to this agent. These data are in agreement with corresponding longitudinal data from the international PROTEKT Global study (1999–2003), which indicate that no significant change in telithromycin susceptibility has been observed since the launch of the drug in some European countries in 2000–2001 [55, 56]. Currently, telithromycin is licensed in the USA for treating community-acquired pneumonia in adults; however, the most recent Infectious Diseases Society of America/American Thoracic Society consensus guidelines on the management of community-acquired pneumonia in adults  do not carry any recommendations regarding the clinical use of telithromycin. Recommendations will be finalized when further evaluation of the safety of telithromycin by the US Food and Drug Administration has been completed.
The findings in this study highlight the need for the judicious use of antimicrobials and the continued monitoring of pneumococcal resistance patterns – in particular, the spread of multiresistant clones. Physicians should take local or regional resistance patterns into consideration when choosing empiric antibacterial treatment for community-acquired RTIs.
In summary, antimicrobial resistance in S. pneumoniae appears to have stabilized in the USA. However, geographic variations remain, and the prevalence of isolates with the combined erm(B) and mef(A) genotype, associated with high-level macrolide resistance (MIC50 > 256 μg/mL) and multidrug resistance, continues to increase. Telithromycin retains potent in vitro activity against S. pneumoniae, including isolates with the combined erm(B)+mef(A) macrolide resistance genotype.
Hoberman A, Marchant CD, Kaplan SL, Feldman S: Treatment of acute otitis media consensus recommendations. Clin Pediatr. 2002, 41: 373-390. 10.1177/000992280204100602.
Ball P: Epidemiology and treatment of chronic bronchitis and its exacerbations. Chest. 1995, 108 (Suppl 2): 43S-52S. 10.1378/chest.108.2_Supplement.43S.
Anon JB, Jacobs MR, Poole MD, Ambrose PG, Benninger MS, Hadley JA, Craig WA, : Antimicrobial treatment guidelines for acute bacterial rhinosinusitis. Otolaryngol Head Neck Surg. 2004, 130 (Suppl 1): 1-45.
Niederman MS, Mandell LA, Anzueto A, Bass JB, Broughton WA, Campbell GD, Dean N, File T, Fine MJ, Gross PA, Martinez F, Marrie TJ, Plouffe JF, Ramirez J, Sarosi GA, Torres A, Wilson R, Yu VL, : Guidelines for the management of adults with community-acquired pneumonia. Diagnosis, assessment of severity, antimicrobial therapy, and prevention. Am J Respir Crit Care Med. 2001, 163: 1730-1754.
Pfaller MA, Jones RN, Doern GV, Kugler K: Bacterial pathogens isolated from patients with bloodstream infections: frequencies of occurrence and antimicrobial susceptibility patterns from the SENTRY antimicrobial surveillance program (United States and Canada, 1997). Antimicrob Agents Chemother. 1998, 42: 1762-1770.
Doern GV, Pfaller MA, Kugler K, Freeman J, Jones RN: Prevalence of antimicrobial resistance among respiratory tract isolates of Streptococcus pneumoniae in North America: 1997 results from the SENTRY antimicrobial surveillance program. Clin Infect Dis. 1998, 27: 764-770. 10.1086/514953
Doern GV, Heilmann KP, Huynh HK, Rhomberg PR, Coffman SL, Brueggemann AB: Antimicrobial resistance among clinical isolates of Streptococcus pneumoniae in the United States during 1999-2000, including a comparison of resistance rates since 1994-1995. Antimicrob Agents Chemother. 2000, 45: 1721-1729. 10.1128/AAC.45.6.1721-1729.2001.
Hoban D, Waites K, Felmingham D: Antimicrobial susceptibility of community-acquired respiratory tract pathogens in North America in 1999–2000: findings of the PROTEKT surveillance study. Diagn Microbiol Infect Dis. 2003, 45: 251-259. 10.1016/S0732-8893(02)00522-9
Karlowsky JA, Thornsberry C, Critchley IA, Jones ME, Evangelista AT, Noel GJ, Sahm DF: Susceptibilities to levofloxacin in Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis clinical isolates from children: results from 2000–2001 and 2001–2002 TRUST studies in the United States. Antimicrob Agents Chemother. 2003, 47: 1790-1797. 10.1128/AAC.47.6.1790-1797.2003
Doern GV, Brown SD: Antimicrobial susceptibility among community-acquired respiratory tract pathogens in the USA: data from PROTEKT US 2000–2001. J Infect. 2004, 48: 56-65. 10.1016/S0163-4453(03)00123-3
Farrell DJ, Jenkins SG: Distribution across the USA of macrolide resistance and macrolide resistance mechanisms among Streptococcus pneumoniae isolates collected from patients with respiratory tract infections: PROTEKT US 2001–2002. J Antimicrob Chemother. 2004, 54 (Suppl 1): I17-I22. 10.1093/jac/dkh312
Farrell DJ, Jenkins SG, Brown SD, Patel M, Lavin BS, Klugman KP: Emergence and spread of Streptococcus pneumoniae with erm (B) and mef (A) resistance. Emerg Infect Dis. 2005, 11 (6): 851-858.
Jenkins SG, Farrell DJ, Patel M, Lavin BS: Trends in anti-bacterial resistance among Streptococcus pneumoniae isolated in the USA, 2000–2003: PROTEKT US years 1–3. J Infect. 2005, 51: 355-363. 10.1016/j.jinf.2005.04.015
Felmingham D, Grüneberg RN: The Alexander Project 1996–1997: latest susceptibility data from this international study of bacterial pathogens from community-acquired lower respiratory tract infections. J Antimicrob Chemother. 2000, 45: 191-203. 10.1093/jac/45.2.191
Hoban DJ, Doern GV, Fluit AC, Roussel-Delvallez M, Jones RN: Worldwide prevalence of antimicrobial resistance in Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in the SENTRY Antimicrobial Surveillance Program, 1997–1999. Clin Infect Dis. 2001, 31 (Suppl 2): S81-S93. 10.1086/320181.
Felmingham D, Reinert RR, Hirakata Y, Rodloff A: Increasing prevalence of antimicrobial resistance among isolates of Streptococcus pneumoniae from the PROTEKT surveillance study, and comparative in vitro activity of the ketolide, telithromycin. J Antimicrob Chemother. 2002, 50: 25-37. 10.1093/jac/dkf089
Marchese A, Gualco L, Cochetti I, Montanari MP, Speciale AM, Musumeci SR, Varaldo PE, Nicoletti G, Schito GC: Antibiotic susceptibility and serotype distribution in Streptococcus pneumoniae circulating in Italy: results of the SEMPRE surveillance study (2000–2002). Int J Antimicrob Agents. 2005, 26: 138-145. 10.1016/j.ijantimicag.2005.04.006
Klugman KP, Lonks JR: Hidden epidemic of macrolide-resistant pneumococci. Emerg Infect Dis. 2005, 1: 802-807.
Kaplan SL, Mason EO, Wald ER, Schutze GE, Bradley JS, Tan TQ, Hoffman JA, Givner LB, Yogev R, Barson WJ: Decrease of invasive pneumococcal infections in children among 8 children's hospitals in the United States after the introduction of the 7-valent pneumococcal conjugate vaccine. Pediatrics. 2004, 113: 443-449. 10.1542/peds.113.3.443
Rzeszutek M, Wierzbowski A, Hoban DJ, Conly J, Bishai W, Zhanel GG: A review of clinical failures associated with macrolide-resistant Streptococcus pneumoniae. Int J Antimicrob Agents. 2004, 24: 95-104. 10.1016/j.ijantimicag.2004.03.008
Farrell DJ, Morrissey I, Bakker S, Felmingham D: Molecular characterization of macrolide resistance mechanisms among Streptococcus pneumoniae and Streptococcus pyogenes isolated from the PROTEKT 1999–2000 study. J Antimicrob Chemother. 2002, 50: 39-47. 10.1093/jac/dkf507
Corso A, Severina EP, Petruk VF, Mauriz YR, Tomasz A: Molecular characterization of penicillin-resistant Streptococcus pneumoniae isolates causing respiratory disease in the United States. Microb Drug Resist. 1998, 4: 325-337.
Farrell DJ, Morrissey I, Bakker S, Morris L, Buckridge S, Felmingham D: Molecular epidemiology of multiresistant Streptococcus pneumoniae with both erm (B)- and mef (A)-mediated macrolide resistance. J Clin Microbiol. 2004, 42: 764-768. 10.1128/JCM.42.2.764-768.2004
Waites K, Brown S: Antimicrobial resistance among isolates of respiratory tract infection pathogens from the Southern United States: data from the PROTEKT US surveillance program 2000/2001. South Med J. 2003, 96: 974-985. 10.1097/01.SMJ.0000091066.74656.6C
Clinical and Laboratory Standards Institute (CLSI) : Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. CLSI document M07-A6. 2003, Wayne, PA: CLSI,
Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing; 15th Informational Supplement. CLSI document M100-S15. 2005, Wayne, PA; CLSI,
Aventis Pharmaceuticals: Ketek® (Telithromycin) tablets. 2005, (accessed November 23, 2005)., http://www.fda.gov/cder/foi/label/2005/021144s004lbl.pdf
Farrell DJ, Morrissey I, Bakker S, Felmingham D: Detection of macrolide resistance mechanisms in Streptococcus pneumoniae and Streptococcus pyogenes using a multiplex rapid cycle PCR with microwell-format probe hybridization. J Antimicrob Chemother. 2001, 48: 541-544. 10.1093/jac/48.4.541
Shackcloth J, Williams L, Farrell DJ: Streptococcus pneumoniae and Streptococcus pyogenes isolated from a paediatric population in Great Britain and Ireland: the in vitro activity of telithromycin versus comparators. J Infect. 2004, 48: 229-235. 10.1016/j.jinf.2004.01.002
Doern GV, Richter SS, Miller A, Miller N, Rice C, Heilmann K, Beekmann S: Antimicrobial resistance among Streptococcus pneumoniae in the United States: have we begun to turn the corner on resistance to certain antimicrobial classes?. Clin Infect Dis. 2005, 41: 139-148. 10.1086/430906
Belongia EA, Knobloch MJ, Kieke BA, Davis JP, Janette C, Besser RE: Impact of statewide program to promote appropriate antimicrobial drug use. Emerg Infect Dis. 2005, 11: 912-920.
Gonzales R, Bartlett JG, Besser RE, Cooper RJ, Hickner JM, Hoffman JR, Sande MA: Principles of appropriate antibiotic use for treatment of acute respiratory tract infections in adults: background, specific aims, and methods. Ann Intern Med. 2001, 134: 479-486.
Niederman MS: Principles of appropriate antibiotic use. Int J Antimicrob Agents. 2005, 26 (Suppl 3): S170-S175. 10.1016/S0924-8579(05)80324-3
Kyaw MH, Lynfield R, Schaffner W, Craig AS, Hadler J, Reingold A, Thomas AR, Harrison LH, Bennett NM, Farley MM, Facklam RR, Jorgensen JH, Besser J, Zell ER, Schuchat A, Whitney CG, Active Bacterial Core Surveillance of the Emerging Infections Program Network: Effect of introduction of the pneumococcal conjugate vaccine on drug-resistant Streptococcus pneumoniae. N Engl J Med. 2006, 354: 1455-1463. 10.1056/NEJMoa051642
Bishai WR: Clinical significance of pneumococcal resistance and factors influencing outcomes. Treat Respir Med. 2005, 4 (Suppl 1): 19-23.
Peterson LR: Penicillins for treatment of pneumococcal pneumonia: does in vitro resistance really matter?. Clin Infect Dis. 2006, 42: 224-233. 10.1086/497594
Klugman KP: Clinical impact of antibiotic resistance in respiratory tract infections. Int J Antimicrob Agents. 2007, 29 (Suppl 1): S6-S10. 10.1016/S0924-8579(07)70004-3
Cunha BA: Clinical relevance of penicillin-resistant Streptococcus pneumoniae. Semin Respir Infect. 2002, 17: 204-214. 10.1053/srin.2002.34686
Schrag SJ, McGee L, Whitney CG, Beall B, Craig AS, Choate ME, Jorgensen JH, Facklam RR, Klugman KP, : Emergence of Streptococcus pneumoniae with very-high-level resistance to penicillin. Antimicrob Agents Chemother. 2004, 48: 3016-3023. 10.1128/AAC.48.8.3016-3023.2004
del Campo R, Cafini F, Morosini MI, Fenoll A, Linares J, Alou L, Sevillano D, Cantón R, Prieto J, Baquero F, Spanish Pneumococcal Network (G3/103): Combinations of PBPs and MurM protein variants in early and high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain. J Antimicrob Chemother. 2006, 57: 983-986. 10.1093/jac/dkl083
Duke T, Curtis N, Fuller DG: The management of bacterial meningitis in children. Expert Opin Pharmacother. 2003, 4: 1227-1240. 10.1517/146565126.96.36.1997
Hoban D, Baquero F, Reed V, Felmingham D: Demographic analysis of antimicrobial resistance among Streptococcus pneumoniae: worldwide results from PROTEKT 1999–2000. Int J Infect Dis. 2005, 9: 262-273. 10.1016/j.ijid.2004.07.008
Whitney CG, Farley MM, Hadler J, Harrison LH, Lexau C, Reingold A, Lefkowitz L, Cieslak PR, Cetron M, Zell ER, Jorgensen JH, Schuchat A, Active Bacterial Core Surveillance Program of the Emerging Infections Program Network: Increasing prevalence of multidrug-resistant Streptococcus pneumoniae in the United States. N Engl J Med. 2000, 343: 1917-1924. 10.1056/NEJM200012283432603
Shortridge VD, Doern GV, Brueggemann AB, Beyer JM, Flamm RK: Prevalence of macrolide resistance mechanisms in Streptococcus pneumoniae isolates from a multicenter antibiotic resistance surveillance study conducted in the United States in 1994–1995. Clin Infect Dis. 1999, 29: 1186-1188. 10.1086/313452
Black S, Shinefield H, Baxter R, Austrian R, Bracken L, Hansen J, Lewis E, Fireman B: Postlicensure surveillance for pneumococcal invasive disease after use of heptavalent pneumococcal conjugate vaccine in Northern California Kaiser Permanente. Pediatr Infect Dis J. 2004, 23 (6): 485-489. 10.1097/01.inf.0000129685.04847.94
Farrell DJ, Klugman KP, Pichichero M: Increased antimicrobial resistance among nonvaccine serotypes of Streptococcus pneumoniae in the pediatric population after the introduction of 7-valent pneumococcal vaccine in the United States. Pediatr Infect Dis J. 2007, 26: 123-128. 10.1097/01.inf.0000253059.84602.c3
Baquero F: Evolving resistance patterns of Streptococcus pneumoniae: a link with long-acting macrolide consumption?. J Chemother. 1999, 11: 35-43.
Lonks JR, Medeiros AA: High rate of erythromycin and clarithromycin resistance among Streptococcus pneumoniae isolates from blood cultures from Providence, R.I. Antimicrob Agents Chemother. 1993, 37: 1742-1745.
Fogarty C, Goldschmidt R, Bush K: Bacteremic pneumonia due to multidrug-resistant pneumococci in 3 patients treated unsuccessfully with azithromycin and successfully with levofloxacin. Clin Infect Dis. 2000, 31: 613-615. 10.1086/313976
Kelley MA, Weber DJ, Gilligan P, Cohen MS: Breakthrough pneumococcal bacteremia in patients being treated with azithromycin and clarithromycin. Clin Infect Dis. 2000, 31: 1008-1011. 10.1086/318157
Waterer GW, Wunderink RG, Jones CB: Fatal pneumococcal pneumonia attributed to macrolide resistance and azithromycin monotherapy. Chest. 2000, 118: 1839-1840. 10.1378/chest.118.6.1839-a
Lonks JR, Garau J, Gomez L, Xercavins M, Ochoa de Echaguen A, Gareen IF, Reiss PT, Medeiros AA: Failure of macrolide antibiotic treatment in patients with bacteremia due to erythromycin-resistant Streptococcus pneumoniae. Clin Infect Dis. 2002, 35: 565-569. 10.1086/341978
Van Kerkhoven D, Peetermans WE, Verbist L, Verhaegen J: Breakthrough pneumococcal bacteraemia in patients treated with clarithromycin or oral β-lactams. J Antimicrob Chemother. 2003, 51: 691-696. 10.1093/jac/dkg116
Garau J: The hidden impact of antibacterial resistance in respiratory tract infection. Clinical failures: the tip of the iceberg?. Respir Med. 2001, 95 (Suppl A): S5-S11. 10.1016/S0954-6111(01)90022-8
Farrell DJ, Felmingham D: Activities of telithromycin against 13, 874 Streptococcus pneumoniae isolates collected between 1999 and 2003. Antimicrob Agents Chemother. 2004, 48: 1882-1884. 10.1128/AAC.48.5.1882-1884.2004
Reinert R, Felmingham D: High sustained activity of telithromycin post-launch in Europe: the PROTEKT study (Years 1–4) [Abstract C2-813]. 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington, DC. October 30-November 2, 2004.
Mandell LA, Wunderink RG, Anzueto A, Bartlett JG, Campbell GD, Dean NC, Dowell SF, File TM, Musher DM, Niederman MS, Torres A, Whitney CG, Infectious Diseases Society of America; American Thoracic Society: Infectious Diseases Society of America/American Thoracic Society consensus guidelines on the management of community-acquired pneumonia. Clin Infect Dis. 2007, 44: S27-S72. 10.1086/511159
We are grateful to our colleagues worldwide for the supply of bacterial isolates as part of the PROTEKT US study. We are also grateful to the G.R. Micro Ltd and CMI PROTEKT teams, who performed the testing. Aventis Pharmaceuticals Inc., a member of the sanofi-aventis Group, is acknowledged for their financial support of the PROTEKT US study.
It may appear that SGJ has a competing interest as it relates to this manuscript as he was an employee of Aventis Pharmaceutical, the sponsor of the PROTEKT project, from May of 2001 until August of 2002 and has served as a consultant as well as a member of the company's Speakers Bureau at various times since leaving the company.
SB has received contract funding, expense reimbursements, and honoraria from sanofi-aventis.
DJF has received research grants and consultancy fees from sanofi-aventis related to telithromycin research, publications, and presentations. Sanofi-aventis are financing the production of this manuscript.
SGJ contributed to the analysis and interpretation of data related to this manuscript and provided clinical isolates to the project for testing. In addition, SGJ was involved in revising the manuscript critically for important intellectual content.
SB actively participated in the overall design and coordination of the study, collection of data, review of the manuscript, and has granted final approval for the publication of the manuscript.
DJF and colleagues at GR Micro Limited undertook the laboratory testing, data collection and analysis, and drafted the paper.
Steven D Brown and David J Farrell contributed equally to this work.
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Jenkins, S.G., Brown, S.D. & Farrell, D.J. Trends in antibacterial resistance among Streptococcus pneumoniae isolated in the USA: update from PROTEKT US Years 1–4. Ann Clin Microbiol Antimicrob 7, 1 (2008). https://doi.org/10.1186/1476-0711-7-1
- Macrolide Resistance
- Penicillin Resistance
- Pneumococcal Resistance
- Acute Bacterial Exacerbation