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Fusion plasmid enhanced the endemic extensively drug resistant Klebsiella pneumoniae clone ST147 harbored blaOXA-48 to acquire the hypervirulence and cause fatal infection



Klebsiella Pneumoniae (Kp) sequence type (ST) 147 has emerged globally and spread rapidly, particularly the extensively drug resistant (XDR) isolates. However, the infections caused by this subtype is rare reported in China for now. The clinical, microbiological and genomic characteristics are unclear.


A systemic retrospective study was conducted in a Chinese tertiary hospital. Clinical information of the infection cases was collected, and whole-genome sequencing and phenotypic experiments were performed on the ST147 isolates. The resistance and virulence genes were identified, and the plasmids harboring these genes were further studied.


Six ST147 isolates from six patients among 720 available clincial Kp isolates were detected. Notably, two isolates, PEKP4035 and PEKP4265, represented both XDR and hypervirulence by acquiring blaOXA-48, blaCTX-M-15 and key virulence genes, iucA + rmpA2, representing no fitness cost and resulting fatal infection. Four of the six ST147 isolates presented with more nucleotide differences, whereas the PEKP4035 and PEKP4265 both isolated from the intensive care unit possessed 20 single nucleotide polymorphisms among one year, indicating the prolonged survive and transmission. Interestingly, the two isolates harbored the same fused plasmid composed of sul2 and iucA + rmpA2, which might be generated by recombination of a plasmid like KpvST101_OXA-48 with the pLVPK plasmid via IS26. Besides, two ~ 70 kb plasmids conferring multiple-drug resistance were also identified among the two isolates, which presented resistance genes including blaOXA-48, blaCTX-M-16, strA and strB. Interestingly, we reported that blaCTX-M-15, a common resistance gene within ST147, has successfully transferred into the chromosome by ISEcp1.


XDR hypervirulent ST147 Kp is emerging, suggesting enhanced surveillance is essential.


Klebsiella pneumoniae (Kp) is an increasingly important pathogen for the capture of causing various infections [1]. In addition to the disturbing resistances to multiple last-line antimicrobial agents, it is also emerging as a key trafficker of various pathogenicity genes due to its high degree of genome plasticity [2]. Kp has successfully evolved into two pathotypes termed the hypervirulent K. pneumoniae (hvKp) and classical K. pneumoniae (cKp) [3].

Both of the two pathotypes presented with distinct characteristics. Previously, the hvKp commonly isolated from the community-acquired liver abscess and was sensitive to most of the antibiotics [4]. From the genetic trait of the hvKp, it is clear that pLVPK/pVir-CR-HVKP4 virulence plasmid was the common contributor [3, 5, 6]. These virulence plasmids were mainly harbored by sequence type (ST) 23 hvKp previously, however, ST11 hvKp harboring similar virulence plasmid has emerged and spread worldwide in recent years. In contrast, cKp featured by high antimicrobial resistance has been prevalent in clinical settings for decades, and was associated with multiple subtypes including ST11, ST258, ST15, etc. The prevalent population of cKp formed a large gene pool for the intra- and inter-species gene exchange and might accelerate the emergence of subtypes with new genetic and phenotypic characteristics.

As the representative cKp, the globe high-risk clone ST147 that initially possessed the mutations within quinolone-resistance determining regions (QRDRs) including S83I in GyrA and S80I in ParC, and blaCTX-M-15 conferring resistance to β-lactams [7]. Recently, ST147 Kp is also notorious for the acquisition of the blaVIM [8] and blaKPC [9] and blaNDM [10,11,12], which is posing great threat to the public health [13]. This subtype is prevalent in the India and North Africa and spread to the worldwide [13], whereas few study reported infections by ST147 in China. Importantly, previous studies demonstrated that hvKp defined as the combination of the iucA, iroB, rmpA, rmpA2 or peg344 is replacing the cKp as the dominant pathotype in the nosocomial and healthcare-associated infection [6] and the cKp is increasingly acquiring the virulence-associated genes in China [6, 14]. Recent study reported that MDR-ST147-hvKp is spread in patients with severe COVID-19 in Italy [15]. However, there is few data focused on the clinical and genomic characteristics of multidrug-resistance (MDR) and hypervirulent ST147 clone in China.

Here, we performed a four-year genome surveillance of Kp in a hospital in China, and discovered novel ST147 Kp characterized a fusion plasmid that encodes both antimicrobial resistance and hypervirulence. Within this ST147 clone, we also observed coharboring of the blaCTX-M-15 and blaOXA-48 conferring extensively drug resistance (XDR), and resulting in fatal infection. Additionally, we found diverse genetic context of the blaCTX-M-15 within ST147 and reported that blaCTX-M-15 was integrated into the chromosome from plasmid via recombination mediated by the ISEcp1.

Materials and methods

Enrolled bacterial isolates and clinical characteristics

From the genomic surveillance conducted within the Peking University Third Hospital, seven hundred twenty Klebsiella. spp were enrolled from 2017 to 2021, among which 6 isolates were examined. All strains were stored at − 80 °C. Strains were initially identified by the MALDI-TOF mass spectrometry and then confirmed by Vitek compact 2 system. The clinical characteristics were obtained from electronic medical records. Basic demographic characteristics, underlying diseases, antimicrobial agent exposure within 90 days, infection type, use of invasive devices and outcomes were collected. Additionally, the Charlson comorbidity index (CCI) was calculated. The protocol for this study was approved by the Peking University Third Hospital Ethics Committee (M2021238), and the Guidelines for Human Experimentation (PRC) were followed throughout. Community-acquired infection (CAI), healthcare-associated (HCAI) and nosocomial infection (HAI) were identified as previously described [6]. Multidrug resistance (MDR) and extensive drug resistance (XDR) were defined as previously described [16].

Antimicrobial susceptibility testing (AST)

AST was performed on the 6 isolates by the Vitek 2 or disk diffusion test. The antimicrobials tested were cefepime (FEP), ceftazidime (CAZ), aztreonam (ATM), imipenem (IPM), meropenem (MEM), piperacillin/tazobactam (TZP), cefperazone-sulbactam (CSL), amikacin (AMK), tobramycin (TOB), colistin (COL), levofloxacin (LVX), ciprofloxacin (CIP), minocycline (MNO), tigecycline (TGC), polymyxin (POL), cefiderocol (FDC), ceftazidime/avibactam (CZA) and trimethoprim/sulfamethoxazole (SXT). AST results were interpreted using 2020 Clinical and Laboratory Standards Institute (CLSI) guideline breakpoints and the tigecycline was followed by the FDA recommendation.

Virulence-associated phenotype and growth curve

HvKp were defined by the presence of some combination of peg-344, iroB, iucA, rmpA, or rmpA2 [17]. Hypermucoviscous phenotype was defined by a positive string test as described previously [18].

Serum killing assays

Serum killing assays of all ST147 strains were conducted as previously described [19]. Briefly, we collected bacterial suspensions and mixed them with healthy human serum at 1:3. The mixture was agitated at 37 °C and the number of the clone was recorded at different time (0 h, 1 h, 2 h, 3 h, respectively). Survival percentages at each different time points were calculated three times and the results were interpreted by previous definition [20].

Biofilm formation capacity

Biofilm formation capacity was evaluated in 96 well plate as previously described [21]. Briefly, bacterial suspensions were inoculated in a 96-well plate followed by 48 h of incubation at 37 °C. Strains were washed with distilled water three times and dyed. Subsequently, the plate wells were incubated with 200 μl of 1% Crystal Violet dye for 20 min and further washed twice. We added 200 μl of 95% ethanol into each well, and the optical density (OD) at 570 nm of the tested isolates was measured and recorded as A. The wells added into LB medium were used as the negative control (Ac). The results were interpreted by the following criteria: (1) No biofilm producer (−), A ≤ Ac; (2) weakly positive (+ +), Ac < A ≤ 2Ac; (3) Moderately positive (+ +), 2Ac < A ≤ 4Ac; and (4) Strongly positive (+ + +), A > 4Ac.

Growth curve

To compare the fitness cost, growth curve was conducted. Briefly, the ST147 strains carrying fusion plasmid cultured overnight and then were diluted to an optical density (OD) 600 of 0.1 and grown at 37 °C in triplicate for 16 h. The OD600 of the cell density was determined every per hour and the growth curve was conducted by the GraphPad Prism version 7.

Galleria mellonella model

The virulence phenotype was evaluated using Galleria mellonella (insects of approximately 300 mg). Strains were adjusted using physiological saline to 1 × 106 CFU/mL and then injected into the Galleria mellonella, as described previously [19]. The survival rate was recorded at 12, 24, 36, 48, 60 and 72 h, respectively and all strains were tested three times.

Plasmid transferability and stability

To clearly understand the plasmid stability, we subcultured strains serially to the 10th passage [22]. The descendants were evaluated by the AST.

For identifying the transferability of the fused plasmid, E. coli J53 was selected as the recipient to perform the conjugation experiments [23]. Briefly, the donor and recipient strain mixed at a ratio of 1:1 and then co-cultured in LB broth overnight. Subsequently, the mixture was spotted on the MacConkey agar containing 320 mg/liter sulfanilamide and 200 mg/liter of sodium azide. After overnight culture, the transconjugants were finally screened. To further understand the transferability of the blaOXA-48 plasmid, we also used the E. coli J53 as the recipient and the meropenem (2 mg/liter) was chosen as the screening antibiotic.

WGS and bioinformatics analysis

The whole genomic of all the ST147 strains was extracted and sequenced by the NovaSeq 6000 platform (paired-end library, 150-bp). The low-quality reads were removed by fastp ( The left clean data were de novo assembled by SPAdes v3.13 and annotated using the Prokka software [24]. resistance genes, virulence genes, insertion sequences (ISs) and plasmid replicon types were identified by ResFinder [25], Virulence Factor Database [26], IsFinder [27], and plasmidFinder [28] database.

To further understand the genomic characteristics of the MDR-hvKp strains, the PEKP4035 and PEKP4265 strains were sequenced by the long-read sequencing platform-MinION to acquire the complete genomes via de novo assembly with a hybrid strategy according to published methods, and assembled using Unicycler [29]. Additionally, to get the genetic context of the blaCTX-M-like within the ST147 strains, we also sequenced the P4 and PEKP4243 to get the complete genomes. Kleborate software was used to determine STs and to detect Yersiniabactin, Colibactin, Aerobactin, Salmochelin, of K and O locus serotypes, associated ICEKp, and the number of AMR classes [30].

The published genome data of the ST147 K. pneumoniae in the GenBank database (accessed on September 1, 2021) were enrolled. Sequencing reads were mapped to the K. pneumoniae HS11286 (accession no.: NC_016845) using bowtie 2 [31] v2.2.8 and the single nucleotide polymorphisms (SNPs) were identified by using Samtools v1.9 and combined according to the reference genome (K. pneumoniae HS11286) using the previously constructed iSNV-calling pipeline ( High-quality SNPs (> 5 reads of mapping quality  > 20) were retained and the recombination sites were further identified by Gubbins [32]. The concatenated sequences of filtered polymorphic sites conserved in all genomes (core genome SNPs, cgSNPs) were used to perform phylogenetic analysis using maximum likelihood method by FastTree software [33].

Nucleotide sequence accession numbers

All the six ST147 genome sequences have been deposited in the NCBI GenBank database under the accession number PRJNA838419.


Emergence of the MDR-ST147-hvKp causing fatal infection

We obtained six ST147 Kp isolates from six patients among 720 available Kp isolates from 454 patients in total. The age of the patient ranged from 53 to 81y. Four cases were defined as HAI and two were HCAI. Five of the six cases (83.3%) were associated with CCI ≥ 3 and two cases (33.3%) suffered from sepsis and shock (Table 1). Four of the six isolates presented MDR phenotypes and two were XDR presenting resistance to quinolones, aminoglycosides, carbapenems and β-lactamase/inhibitors (Table 1 and Additional file 1: Table S1). All the six isolates showed no hypermucoviscosity (Table 2). The biofilm formation capacity presented within all the isolates and no significant fitness cost was shown among all the isolates (Additional file 1: Figure S1, Table 2). Four of the six isolates belonged to KL64 serotype and all of the six isolates displayed sensitivity to the serum (Table 2 and Additional file 1: Figure S2). Interestingly, four ST147 isolates (66.7%) represented resistance to tigecycline, greatly challenging the clinical practice (Additional file 1: Table S1).

Table 1 Clinical characteristics of the ST147 Kp strains
Table 2 Microbiological and genomic characteristics of the ST147 strains

Importantly, the two XDR serotype KL64 isolates, PEKP4035 and PEKP4265, also showed hypervirulence in the Galleria mellonella model but without hypermucoviscous phenotype. The two patients infected by the two isolates were critically ill (SOFA > 6) and died within 7 days after admission (Table 1, Additional file 1: Figure S3). Therefore, the two isolates displayed both carbapenem-resistance and hypervirulence phenotype, and were defined as CR-hvKp.

Phylogenetic analysis, differences of virulence gene and resistance gene profiles

To assess the phylogenetic relationship of our ST147 isolates, we collected 473 published genome sequences of ST147 Kp isolates in addition to the 6 we sequenced. A maximum likelihood phylogenetic tree was constructed using all the 479 genomes based on 16,994 hqSNPs. Our six ST147 isolates were clustered in three distinct branches (Additional file 1: Figure S4). The two XDR hvKp isolates were clustered together with other five published ST147 hvKp genomes. The isolation interval of PEKP4035 and PEKP4265 was almost one year, and their genomes differed by 20 hqSNPs, suggesting the XDR-hvKp might be persistently existed in the hospital (Additional file 1: Table S2). Most of the 479 ST147 genomes (462, 96.2%) carried resistance genes against three or more classes of antimicrobial agents and were denoted as MDR. Forty-six (9.6%) carried at least one of the five virulence associated genes including peg-344, iroB, iucA, rmpA, and rmpA2, and were denoted as hvKp, all of which were also MDR. In addition, these hvKp were clustered with cKp in different branches, indicating multiple horizontal transfer events of virulence genes and acquisition among MDR ST147 cKp, by which the MDR-ST147-hvKp evolved (Additional file 1: Figure S4).

The virulence genes including iucABCDiutA, peg-589 and rmpA2 were identified in PEKP4035 and PEKP4265, which were in line with the hypervirulence phenotype. In contrast, none virulence gene was discovered in other four cKp. The two CR-hvKp isolates carried the same resistance genes but the four cKp isolates represented different resistance gene profiles. The resistance genes including blaOXA-48, blaCTX-M-15, blaCTX-M-16, sul2, arr-2, strA-strB, ant(3'')Ih and rmtF were identified in the two CR-hvKp and in line with their XDR phenotype, and none of these resistance genes were identified in the four ST147 cKp. Only three resistance genes were shared by the two CR-hvKp isolates and four cKp isolates, including oqxA-oqxB, blaSHV-11 and fosA. These results indicated different phylogenetic features, resistance gene and virulence gene profiles of the two ST147 CR-hvKp compared with ST147 cKp in our hospital (Fig. 1).

Fig. 1
figure 1

Phylogenetic analysis of ST147 Kp in our hospital correlate with the distribution of resistance genes (red-white heatmap), virulence genes (blue-white heatmap) and plasmid types (green-white heatmap)

Fusion plasmid encoding both virulence genes and resistance genes

We then obtained the complete sequences of the two XDR-ST147-hvKp isolates, PEKP4035 and PEKP4265. Two plasmids, pPEKP4035-265 and pPEKP4265-269, which were highly similar with each other only differed by small indels (Fig. 2), were found to encode the virulence genes, including iucABCDiutA, peg-589 and rmpA2. The plasmid pPEKP4035-265 was 265-kb in size and pPEKP4265-269 was 269-kb in size, and both comprised of the IncFIB and IncHI1B types of plasmid replicons. The large difference was a 7.5-kb insertion identified in pPEKP4265-269 encoding hypothetical proteins. The AMR gene sul2 was also identified in the two plasmids. These plasmids were highly similar with plasmid KpvST101_OXA-48 (accession number: CP031372, 93.7% coverage with 98.3% identity) (Fig. 3). The ~ 70-kb fragment on pPEKP4265-269 encoding the virulence genes was found to replace the ~ 8-kb resistance regions with the genetic context of IS26-armA-ISEc29-msr(E)-IS26 on plasmid KpvST101_OXA-48. This fragment was highly similar with the corresponding regions of virulence plasmid pLVPK (96% ~ 99% identity), and an IS26 element was also found in the right side of this fragment (Fig. 3). Therefore, this plasmid might be generated by recombination of a plasmid like KpvST101_OXA-48 with the classic virulence plasmid of hvKp via IS26.

Fig. 2
figure 2

Comparison between fused plasmid pPEKP4265-269 encoding sul2 and iucA + rmpA2 found in this study and similar plasmids found in the online NCBI database. The outmost circle of arrows indicated the genes of reference plasmid pPEKP4265-269 used for comparison (red: AMR genes; green: integrase, recombinase, and transposase genes; purple: transfer associated genes; orange: gray: other functions)

Fig. 3
figure 3

Linear plasmid sequence comparison of plasmid pLVPK, pPEKP4035-265, pPEKP4265-269 and KpvST101_OXA-48. The matched regions between two sequences are displayed by light blue blocks. The arrows represent the genes related to resistance and transfer (red: AMR genes; green: integrase, recombinase, and transposase genes; purple: transfer associated genes; orange: virulence associated genes; gray: other functions)

The similar fusion plasmids were also identified among published data in NCBI, like phvKpST147_NDM-29, KpvST147B_SE1_1_NDM, pSI0739-ARMA-Vir, an unnamed plasmid of strain IR5086, and a 308-kb contig of strain CriePir29 (Fig. 4). These plasmids encoded a number of resistance genes, particularly in phvKpST147_NDM-29 the blaNDM-7 gene was identified. The resistance regions on these plasmids comprised of resistance genes and multiple types of ISs including IS26, which were well known as common recombination sites for resistance elements. Therefore, this type of fusion plasmid encoded both virulence genes and resistance islands, and had the potential ability to acquire more resistance genes.

Fig. 4
figure 4

Linear plasmid sequence comparison of plasmids similar with pPEKP4265-269. The matched regions between two sequences are displayed by light blue blocks. The arrows represent the genes related to resistance and transfer (red: AMR genes; green: integrase, recombinase, and transposase genes; purple: transfer associated genes; orange: virulence associated genes; gray: other functions)

Co-harboring of virulence plasmid and MDR plasmid

In addition to the virulence plasmids, a ~ 70-kb MDR plasmid was also identified among the two ST147 MDR hvKp, named pPEKP4035-70 and pPEKP4265-70. This plasmid comprised of the IncL/M type of plasmid replicon, and resistance genes including blaOXA-48, blaCTX-M-16, strA and strB (Additional file 1: Figure S5). This plasmid accounted for the XDR phenotype including resistances to carbapenems and β-lactams. The blaOXA-48 was located within the resistance element comprised of IS10A, IS1R, blaOXA-48 and IS10A, and the other three genes were located within the resistance element comprised of ISEcp1, IS26, blaCTX-M-16, strA and strB. The fusion plasmid encoding virulence genes and resistance gene sul2, and the IncL/M type plasmid encoding resistance gene blaOXA-48 did not transfer into the recipient in the conjugation assays, and could persistently exist during continuous passages.

Diverse genetic context of the bla CTX-M-15 within the ST147 isolates

The PEKP4035 and PEKP4265 both carried blaCTX-M-15. Previous study reported that blaCTX-M-15 located on IncF or IncR plasmids [13]. A 152-kb IncFII type plasmid, pPEKP4265-152, were discovered in the isolate PEKP4265 and harbored blaCTX-M-15 adjacent to an ISEcp1 element (Additional file 1: Figure S6). Three other resistance genes, arr-2, aac(6')-IId and rmtF were also identified in a resistance region 50-kb away from blaCTX-M-15. The similar IncFII plasmid, pPEKP4035-124, was also found in the other XDR hvKp isolate PEKP4035. Interestingly, blaCTX-M-15 was absent in plasmid pPEKP4035-124, and was identified in the chromosome of PEKP4035. By the comparative analysis, we found that a 27.8-kb fragment encoding blaCTX-M-15 in pPEKP4265-152 was lost in pPEKP4035-124 and inserted into the chromosome of PEKP4035. These results indicated the recombination of this fragment between chromosome and plasmid caused the relocation of blaCTX-M-15, which might be mediated by the ISEcp1 (Fig. 5).

Fig. 5
figure 5

Linear plasmid sequence comparison of plasmid pPEKP4035-124, pPEKP4265-152 and corresponding regions on the chromosome of PEKP4035. The matched regions between two sequences are displayed by light blue blocks. The arrows represent the genes related to resistance and transfer (red: AMR genes; green: integrase, recombinase, and transposase genes; purple: transfer associated genes; orange: gray: other functions)


To the best of our knowledge, this is the first report for theemergence, clinical and genomic characteristics of the MDR-ST147-hvKp in a large hospital in China. Although the ST147 accounts for less than one percent during four years in the study in the hospital, more than half of the MDR-ST147 isolates presented with tigecycline non-susceptibility and one third MDR-ST147 isolates harbored key virulence genes iucA and rmpA2. What makes things worse is that XDR-ST147-hvKp emerged and resulted fatal infection. More importantly, it is noted that the fusion plasmid co-encoded the resistance and hypervirulence phenotype is emerging, alarming that it might enhance the MDR or XDR ST147 clone to confer with the hypervirulence and then transmit within the hospital environment, posing great threat to the public health. Additionally, we reported that the genomic context of the blaCTX-M-15 is diverse and firstly found that blaCTX-M-15 located in the chromosome within the ST147 isolates, forming stable MDR phenotypes. These findings are concerning and have significant implications for patient care and outcomes.

ST147 is notorious as the high risk cKp with high resistance globally, which is special endemic in India, Italy, Greece, and North African countries [13]. Previous studies demonstrated that ST147 harboring various resistance genes triggered various outbreaks [34, 35]. However, few studies focused on the ST147 in China. Wang, et al. [36] firstly reported an outbreak of a nosocomial NDM-1-Producing ST147 Kp in China and all the patients presented with no history of traveling to endemic regions or receiving any healthcare abroad. Additional studies from our country also unclarified the recent traveling history of the enrolled patients who infected with ST147 [37], suggesting that the origin and transmission is not clear. It seems that ST147 isolated from the Europe tended to acquire blaNDM-like [10, 11, 34]. However, blaOXA-48, not the blaNDM-like, regarded as the predominant carbapenemase genes within the ST147 isolates in our hospital.

It is noted that the clinical characteristics of the ST147 clone also needed for further study [13]. Previous studies reported that ST147 mostly isolated from the rectal swabs, which is highly associated with colonization, not infection [10, 11]. The detection rate of the ST147 in our hospital is lower than the classical predominant cKp, ST11 [38]. The patients were middle-aged and suffered from various underlying diseases, and all the isolates presented resistances to all first-line agents, similar to the previous studies [11]. Importantly, the emergence of the XDR-ST147-hvKp presented with tigecycline non-susceptibility triggered fatal infection in the hospital, greatly challenging the clinical practice.

In the study, the two XDR-hvKp presented with KL64 and resulted fatal infections within 7 days, which was in line with the hypervirulence in vivo model. Interestingly, the two isolates did not possess hypermucoviscosity phenotype, similar to previous study [11], suggesting that regulation of the hypermucoviscosity phenotype formation needs further studies. Previous study demonstrated that thickness of the capsule might be serve as physical barrier for acquiring external genes [39]. ST147 has divided into two main clades with distinct KL subtypes [40]. The predominant is KL64, firstly emerged in 1994 and rapidly disseminated worldwide, and highly associated with various carbapenemases [40]. The other is KL10, predominantly found in Asian countries, closely related with blaNDM-like and blaOXA-48 [40]. Interestingly, two XDR ST147 isolates we obtained presented with KL64 acquired blaOXA-48 and key virulence genes (iucA + rmpA2), and conferred with hypervirulence and carbapenem resistant phenotype.

Fusion plasmid plays great roles for the convergence of the resistance and hypervirulence within either cKp or hvKp strains [41, 42]. In the study, fusion plasmids of the PEKP4035 and PEKP4265 might be generated by multi-recombination events of a plasmid like KpvST101_OXA-48 with the pLVPK via IS26, not the pVir-like plasmid. Previous study reported that pLVPK-like plasmid acquired blaKPC-2 within ST23 Kp mediated by the IS26 element [43]. Our previous study also demonstrated that IS26 contributed to the formation of the MDR-hvKp [44]. Interestingly, the two XDR-hvKp strains co-harbored fusion plasmids that possessed the backbone of the KpvST101_OXA-48 plasmid and IncL/M plasmid that presented with blaOXA-48, suggesting that continuous dynamic monitoring of the fusion plasmids by WGS is essential. During about one year, PEKP4035 and PEKP4265 were detected in the ICU department, and different by 20 SNPs. One possible reason is that the XDR and hypervirulence triggered the prolonged transmission. The other possibility is that multiple plasmids harbored blaOXA-48, blaCTX-M-like, rmtF and iucA + rmpA2 could co-transfer into other Kp strains. These hypothesis need large-scale systematic clinical and genomic surveillance to further understand the transmission route.

The global dissemination of ST147 occurred primarily driven by QRDR mutations and the acquisition of blaCTX-M-15 located on IncF or IncR plasmids [13, 45]. The location of blaCTX-M-15 in the chromosome has already been reported in K. pneumoniae and other Enterobacterales [46]. In the study, we found that the two isolates PEKP4265 and PEKP4035 possessed blaCTX-M-15. Interestingly, the genetic contexts of the blaCTX-M-15 in the two isolates are different. One is located in the IncFII plasmid and the other is in the chromosome. These results indicated that blaCTX-M-15 might be successfully harbored by both of the plasmid and chromosome in ST147 K. pneumoniae and contribute to the dissemination of ST147.


In summary, XDR-ST147-hvKp is emerging and caused fatal infection. Whole genomic sequencing enhanced the surveillance of genetic context of the resistance genes and the hybrid plasmids converged the resistance and virulence.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.


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This work was supported by National Natural Science Foundation of China (82200012) and Beijing Key Clinical Specialty Program (010071).

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Authors and Affiliations



ML and NS designed the study, supervised the whole project and revised the manuscript. CL and PD performed the bioinformatic analysis, data collection and contributed in writing and revising the manuscript. PY performed experiments and collected clinical data and revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Ming Lu or Ning Shen.

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All methods have been performed in accordance with the Declaration of Helsinki. The protocol for this study was approved by the Peking University Third Hospital Medical Science Research Ethics Committee (M2021545). Due to the retrospective nature of the study, the need for approval was waived by the Peking University Third Hospital Medical Science Research Ethics Committee, and all the patient data enrolled in this study were anonymized.

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The authors declare that they have no competing interests.

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Supplementary Information

Additional file 1: Table S1.

AST of the ST147 clone and its passage. Table S2. Pairwise SNP comparison between ST147 strains in this study. The numbers depict differences in SNPs exhibited by each strain pair. Figure S1. Growth curves of the ST147 strains. Figure S2. Serum killing assays of the ST147 isolates. Figure S3. Virulence of the MDR-ST147-hvKp evaluated by the Galleria mellonella model. Figure S4. Phylogenetic analysis of ST147 Kp in our hospital and the published ST147 genomes from GenBank database. The red dots adjacent to the tips of the tree represent MDR strains, the outer two rings of color bars represent resistance score and virulence score calculated by kleborate software, and the outer blue dots represent hvKp strains based on the presence of the five virulence associated genes including peg-344, iroB, iucA, rmpA, and rmpA2. Figure S5. Comparison between plasmid pPEKP4265-70 encoding blaOXA-48 found in this study and similar plasmids found in the online NCBI database. The outmost circle of arrows indicate the genes of reference plasmid pPEKP4265-70 used for comparison (red: AMR genes; green: integrase, recombinase, and transposase genes; purple: transfer associated genes; orange: gray: other functions). Figure S6. Comparison between plasmid pPEKP4265-152 encoding blaCTX-M-15 found in this study and similar plasmids found in the online NCBI database. The outmost circle of arrows indicate the genes of reference plasmid pPEKP4265-152 used for comparison (red: AMR genes; green: integrase, recombinase, and transposase genes; purple: transfer associated genes; orange: gray: other functions).

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Liu, C., Du, P., Yang, P. et al. Fusion plasmid enhanced the endemic extensively drug resistant Klebsiella pneumoniae clone ST147 harbored blaOXA-48 to acquire the hypervirulence and cause fatal infection. Ann Clin Microbiol Antimicrob 22, 11 (2023).

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