Table 2 Primers used in the amplification of selected carbapenemase genes
Name | Nucleotide sequence (5′ → 3′) | Product size (bp) | Location | References |
|---|---|---|---|---|
OXA-23-like | F- GATCGGATTGGAGAACCAGA | 501 | blaOXA-23 | [31] |
R- ATTTCTGACCGCATTTCCAT | ||||
OXA-24-like | F- GGTTAGTTGGCCCCCTTAAA | 246 | blaOXA-24 | [31] |
R- AGTTGAGCGAAAAGGGGATT | ||||
OXA-51-like | F- TAATGCTTTGATCGGCCTTG | 353 | blaOXA-51 | [31] |
R- TGGATTGCACTTCATCTTGG | ||||
OXA-58-like | F- AAGTATTGGGGCTTGTGCTG | 599 | blaOXA-58 | [31] |
R- CCCCTCTGCGCTCTACATAC | ||||
IMPa | F- GGAATAGAGTGGCTTAAYTCTC | 232 | blaIMP | [32] |
R- GGTTTAAYAAAACAACCACC | ||||
VIM | F- GATGGTGTTTGGTCGCATA | 390 | blaVIM | [32] |
R- CGAATGCGCAGCACCAG | ||||
SPM | F- AAAATCTGGGTACGCAAACG | 271 | blaSPM | [32] |
R- ACATTATCCGCTGGAACAGG | ||||
NDM | F- GGTTTGGCGATCTGGTTTTC | 621 | blaNDM | [32] |
R- CGGAATGGCTCATCACGATC | ||||
GES | F-ATGCGCTTCATTCACGCAC | 863 | blaGES | [33] |
R-CTATTTGTCCGTGCTCAGGA | ||||
GES | F- CGGTTTCTAGCATCGGGACACAT | 263 | blaGES | [34] |
R- CCGCCATAGAGGACTTTAGCMACAG | ||||
Probe: Quasar 705-CGACCTCAGAGATACAACTACGCCTATTGC-BHQ2 | ||||
NDM-1 | F- ATTAGCCGCTGCATTGAT | 154 | blaNDM | [35] |
R- CATGTCGAGATAGGAAGTG | ||||
Probe: FAM-CTG[+ C]CA [+ G]AC [+ A]TT [+ C]GG TGC-TAMRA | ||||
KPC | F- GATACCACGTTCCGTCTGG | 246 | blaKPC | [36] |
R- GCAGGTTCCGGTTTTGTCTC | ||||
Probe: FAM-AGCGGCAGCAGTTTGTTGATTG-3′–BHQ1 |