A fatal case of Nocardia otitidiscaviarum pulmonary infection and brain abscess: taxonomic characterization by molecular techniques
© Pelaez et al; licensee BioMed Central Ltd. 2009
Received: 04 November 2008
Accepted: 14 April 2009
Published: 14 April 2009
We report on a rare case of pulmonary Nocardiosis and brain abscess caused by Nocardia otitidiscaviarum in an elderly woman with chronic obstructive pulmonary disease. Taxonomic identification involved phenotypic testing, restriction fragment length polymorphism (RFLP), and complete 16S rRNA gene sequencing.
An 85-year-old female living in a rural area with a history of hypertension, coronary disease, and severe to very severe chronic obstructive pulmonary disease (COPD) was admitted to hospital with increasing dyspnea, cough, whitish sputum, and pleuritic chest pain on her right side. She had been on treatment with prednisone for eighteen months, after which treatment was switched to deflazacort (15 mg/day) two months prior to suffer two COPD exacerbations. At admission, the patient presented a Barthel Index of 60; her temperature was 37.3°C with 89% oxygen saturation, while breathing ambient air. Spirometric examination demonstrated severe airway obstruction with a forced vital capacity (FVC) of 1.2 L, forced expiratory volume in 1 s (FEV1) of 0.53 L, and FEV1/FVC of 44%. She had diminished breathing sounds and inspiratory crackles in the lower half of both lungs. Physical and neurological examinations were unremarkable.
Repeated sputum and blood cultures were negative. Pleural fluid was obtained by puncture and microscopic examination revealed numerous polymorphonuclear leucocytes and modified acid fast positive, filamentous-branched, gram-positive rods. The growth from the specimen, which had been cultured on blood and chocolate agar plates displayed dry, irregular, adherent, white colonies after 72 h incubation at 37°C. The isolate was initially characterized on the basis of routine phenotypic test results such as colonial morphology, positive Gram stain; positive catalase test; growth in nutrient broth with lysozyme; reduction of nitrate; hydrolysis of xanthine, hypoxanthine and urea, and the inability to hydrolyze casein and tyrosine . The enzymatic profile obtained by API ZYM (BioMerieux, Marcy l'Etoile, France) exhibited negative reactions to valine arylamidase, N-acetyl-β-glucosaminidase, and α-mannosidase. Antimicrobial susceptibility testing was performed simultaneously by Kirby Bauer disc diffusion  and E-tests (AB Biodisk, Sweden), following the protocol described by Martin et al., 2004, for other actinomycetes (Streptomyces) . A suspension of the microorganism, with turbidity equivalent to 1.0 McFarland standard, was inoculated (150 μl/plate) by confluent swabbing on Mueller-Hinton agar (MHA) plates. A maximum of two E-test strips were applied to each MHA plate, which were then incubated at 35°C for 3 days. MICs were determined according to manufacturer's guidelines. Susceptibility results were as follows: penicillin G > 256 μg/ml; ampicillin > 256 μg/ml; imipenem 0.75 μg/ml; vancomycin 32 μg/ml; erythromycin 64 μg/ml; linezolid 0.5 μg/ml; trimethoprim/sulphamethoxazole (TMP/SMX) 0.75 μg/ml; gentamycin 0.25 μg/ml; amikacin 0.75 μg/ml, and ciprofloxacin 0.50 μg/ml.
After a tentative diagnosis of pulmonary nocardiosis, the patient was treated with TMP/SMX (2400/480 mg/day) and imipenem (4 g/day iv) for ten days. However, due to tonic-clonic convulsions, the imipenem was switched to oral linezolid 600 mg/12 h and dexamethasone 10 mg-bolus followed by 4 mg iv every 6 h. The patient developed anemia and thrombocytopenia after one week, and linezolid was therefore discontinued. The patient expired one month after admission. At autopsy, specimens from pleural fluid and the cerebral abscess were taken and cultured. Nocardia growth was observed and confirmed by subculture and microscopy: colonies had a brown velvety appearance, branching filaments were visible microscopically (0.5–1.2 μm diameter), that often fragment into rod- to coccoid shaped forms.
In order to characterize the Nocardia isolates from pleural fluid and the brain abscess, the 16S rRNA gene (1500 bp) was amplified. The 16S rRNA gene from both isolates was amplified using the primers pA: 5'-AGAGTTTGATCCTGGCTCAG-3' and pH: 5'-AAGGAGGTGATCCAGCCGCA-3'. PCR was performed using a reaction mixture containing 1× PCR buffer; 0.2 mM (each) dATP, dTTP, dGTP, and dCTP; 0.2 mM of each primer; 2 U of EcoTaq DNA polymerase (Ecogen, TaKaRa Biotechnology, Japan), and ultrapure water to a final volume of 50 μl. PCR amplification was performed in a DNA thermal cycler (MJ Research Inc., MA, USA) and included initial denaturation at 94°C for 5 min, followed by 30 cycles of amplification (94°C for 1 min, 57°C for 1 min, and 72°C for 2 min) followed by a 10-min extension at 72°C. PCR products were visualized by 0.8% agarose gel electrophoresis and ethidium bromide staining. Bands corresponding to 16S rRNA gene were purified using the GFX™ PCR DNA and Gel Band Purification Kit (Amersham Biosciences, NY, USA) following the manufacturer's instructions. In order to obtain preliminary information about both isolates the 16S rRNAs were analyzed by restriction fragment length polymorphism (RFLP) with Hae III and Alu I restriction endonucleases (TaKaRa Biotechnology) . An aliquot of each of the PCR-amplified 16S rRNA genes was digested at 37°C for 4 h and the resulting restriction fragments were separated on a 3% agarose electrophoresis gel. The restriction patterns obtained after digestion were identical in both strains (data not shown), strongly suggesting that the two isolates corresponded to the same microorganism. After that, and with the aim of confirming or refuting, the identity between both isolates, the full 16S rRNA amplified genes were sequenced. Sequencing was performed using the pA and pH primers (see above) and the BigDye® Terminator v3.1 Cycle Sequencing Ready Reaction Kit using an ABI Prism™ 3100 Genetic Analyzer (PerkinElmer Inc., NY, USA) to electrophorese the sequence product. The Sequence Scanner v1.0 (Applied Biosystems Foster City, CA, USA) and BioEdit (v5.0.6) programs were used to analyze the sequence data thereby derived. Sequencing data were queried against sequences in the GenBank http://www.ncbi.nlm.nih.gov and EMBL http://www.ebi.ac.uk/embl/index.html databases using the BLASTN program. The partial sequences of the 16S rRNA genes from these isolates (1,450 bp; GenBank accession no. EU203569 and EU203570) shared maximum identity (100%) with the 16S rRNA from Nocardia otitidiscaviarum strain DSM 44565 (GenBank accession no. AF430068), 99.9% identity with N. otitidiscaviarum type strain DSM 43242T (GenBank accession no. AF430067) and 98% with other Nocardia species such as Nocardia nova (GenBank accession no. AB162789). An alignment of the 16S rRNA sequences from both isolates with DSM 43242T type strain displayed a 99.9% identity (only one base difference). This allowed the identification of the pleural fluid and the cerebral abscess isolates as N. otitidiscaviarum.
Nocardiosis is an acute or chronic infection caused by aerobic, soil inhabiting actinomycetes of the Nocardia genus. Patients with nocardiosis are generally debilitated by other underlying diseases such as cancer, pulmonary tuberculosis, hematological malignancies, immunosuppressant therapy, or HIV infection [5, 6]. Like the case we report, some patients with pulmonary nocardiosis have COPD and have often been treated with long-term, high-dose corticosteroid therapy. Brain abscess usually arises from hematogenous spread from a primary lung infection. Nocardial brain abscesses are uncommon (< 2%), but are the most common non-pulmonary site in disseminated nocardiosis and have been recorded in between 10 and 15% of patients with this infection . To date and including our case, only six cases of central system nervous infection by N. otitidiscaviarum have been reported in the literature [5, 7–10], without any taxonomic characterization using molecular techniques such as 16S rRNA gene sequencing.
N. otitidiscaviarum was first recognized in samples taken from a Sumatran cavy or guinea pig with ear disease . Infections due to N. otitidiscaviarum appear to be quite rare (3%) compared to those caused by other Nocardia species and no published reports of human infection appeared until the mid 1960s. The first systemic infection in humans was reported in 1974 [7, 12]. This low incidence has been attributed either to reduced pathogenicity or its lower prevalence in soil . It is also possible that N. otitidiscaviarum infections are more common, but not properly diagnosed, as postulated by Patel et al., . This has also been the case with other Nocardia species [14, 15].
The diagnosis of nocardiosis, based on conventional phenotypic characterization of the strains (chemotaxonomic, morphology, etc) is very time consuming compared to genotypic methods . Sequence analysis of the 16S rRNA gene has become the "gold standard" for identifying nocardiae isolates at the species level . In our study, PCR amplification of the complete 16S rRNA gene and subsequent RFLP analysis enabled us to obtain band patterns for comparison of the different isolates from the same patient. A 16S rRNA phylogenetic tree has shown that the N. otitidiscaviarum species isolated in this work (accession no. EU203569 and EU203570) are grouped with other clinical isolates of N. otitidiscaviarum (see above and Figure 2), which correspond to well characterized collection strains (accession no. AF430068 and AF430067). The two isolates obtained from the reported patient exhibited identical 16S rRNA gene sequences to the 16S rRNA gene of N. otitidiscaviarum strain DSM 44565 (accession no. AF430068) previously described as genotype II of N. otitidiscaviarum . Also, the comparison between 16S rRNA from both isolates and type strain (accession no. AF430067) resulted in a 99.9% identity. Therefore, both isolates can be named as N. otitidiscaviarum, although an unambiguous identification to the species level will require additional molecular biological and phenotyping experiments. The other N. otitidiscaviarum clinical species reported by other authors belong to different clusters: one comprised of four members (accesion no. Z82237, Z82234, Z82238, Z82239) and another one consisting of two (AB201303, AB201298) (Figure 2). These clusters are separated from the cluster which harbour the N. ottidiscaviarum type strain, suggesting that more in deep taxonomic studies of the clinically significant species are necessary .
TMP/SMX or minocycline are the most frequently used antibiotics for nocardiosis treatment in localized or mild cases. Combination therapy with a carbapenem or a third-generation cephalosporin with or without amikacin is generally recommended for severely ill patients or for those with central system nervous involvement, in whom mortality is close to 50% when sulfonamide monotherapy is received . Nonetheless, clinical experience with linezolid in nocardiosis is limited . The MIC90 for linezolid was ≤ 4 μg/ml against isolates of N. otitidiscaviarum . This agent was successfully used to treat individuals with nocardiosis, including patients who had failed therapy with other antimicrobials. These cases have highlighted the excellent penetration of linezolid into cerebrospinal fluid after intravenous administration of the drug every 12 h . The overall mortality rate for nocardial brain abscess is 47.8% for N. asteroides infection . In all reported cases of N. otitidiscaviarum brain abscess, including our case, the patients died despite surgical and/or antibiotic therapy [7–10] except one case  that survived following surgical excision and antibiotic treatment with imipenem and TMP/SMX. Overall mortality in these six cases was 83.3%. In the case we report there are, several factors that may have contributed to the failure to cure the disease, such as deterioration of this patient due to underlying conditions (the patient's age and chronic diseases), as well as the development of severe intolerance to antimicrobials. Aspiration or excision of the abscess would have been necessary to achieve a positive outcome.
In summary, to the best of our knowledge this is the first reported case of N. otitidiscaviarum isolated from a patient with pulmonary nocardiosis and brain abscess described and characterized by complete 16S rRNA gene sequencing.
We did informed consent for the family's patient about publishing the manuscript to Annals of Clinical Microbiology and Antimicrobials. Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
This work was supported by grant MEC-06-BI02006-15336-C04-02 from Ministerio de Ciencia e Innovación, Spain and UNOV-08-MB-3 de la Universidad de Oviedo, Spain.
The authors would like to acknowledge Professor Jesus Sanchez (Area Microbiologia, Universidad de Oviedo) for their critical reading of the manuscript and help with phylogenetic analysis. A.I.P. was supported by grant CENIT-07-CLEAM, Ministerio de Industria, Turismo y Comercio (CDTI), Spain. A.M. was supported by grant MEC-07- BIO2007-66313 from the DGI, Subdireccion General de Proyectos de Investigacion, MEC, Spain, and M.M.G.S. was supported by grant MEC-06-BIO2006-1533-C04-02. We also thank Priscilla Chase for revising the text.
- Brown JM, McNeil MM, Desmond EP: Nocardia, Rhodococcus, Gordona. Actinomadura, Streptomyces, and other actinomycetes of medical importance. Manual of Clinical Microbiology. Edited by: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH. 1999, 370-398. ASM Press, Washington DC, 7, Google Scholar
- Biemer JJ: Antimicrobial susceptibility testing by the Kirby-Bauer disc diffusion method. Ann Clin Lab Sci. 1973, 3: 135-140.PubMedGoogle Scholar
- Martín MC, Manteca A, Castillo ML, Vázquez F, Méndez FJ: Streptomyces albus isolated from a human actinomycetoma and characterized by molecular techniques. J Clin Microbiol. 2004, 42: 5957-5960. 10.1128/JCM.42.12.5957-5960.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Broda DM, Musgrave DR, Bell RG: Use of restriction fragment length polymorphism analysis to differentiate strains of psychrophilic and psychrotropic clostridia associated with blown pack' spoilage of vacuum-packed meats. J Appl Microbiol. 2000, 88: 107-116. 10.1046/j.1365-2672.2000.00925.xView ArticlePubMedGoogle Scholar
- Duran E, Lopez L, Martinez A, Comuñas F, Boiron P, Rubio MC: Primary brain abscess with Nocardia otitidiscaviarum in an intravenous drug abuser. J Med Microbiol. 2001, 50: 101-103.PubMedGoogle Scholar
- Maraki S, Scoulica E, Nioti E, Tselentis Y: Nocardial infection in Crete, Greece: Review of fifteen cases from 2003 to 2007. Scand J Infect Dis. 2009, 41: 122-127. 10.1080/00365540802651905View ArticlePubMedGoogle Scholar
- Causey WA, Arnell P, Brinker J: Systemic Nocardia caviae infection. Chest. 1974, 65: 360-362.View ArticlePubMedGoogle Scholar
- Arroyo JC, Nichols S, Carroll GF: Disseminated Nocardia caviae infection. Am J Med. 1977, 62: 409-412. 10.1016/0002-9343(77)90839-7View ArticlePubMedGoogle Scholar
- Bradsher RW, Monson TP, Steele RW: Brain abscess due to N. caviae. Report of a fatal outcome with abnormal phagocyte function. Am J Clin Pathol. 1982, 78: 124-127.PubMedGoogle Scholar
- Talwar P, Chakrabarti A, Ayyagari A, Nayak N, Khosla VK, Minz M, Yadav RVS: Brain abscess due to Nocardia. Mycopathologia. 1989, 108: 21-23. 10.1007/BF00436779View ArticlePubMedGoogle Scholar
- Brown-Elliott BA, Brown JM, Conville PS, Wallace RJ: Clinical and laboratory features of the Nocardia spp. based on current molecular taxonomy. Clin Microbiol Rev. 2006, 19: 259-282. 10.1128/CMR.19.2.259-282.2006PubMed CentralView ArticlePubMedGoogle Scholar
- Beaman BL, Burnside J, Edwards B, Causey W: Nocardial infections in the United States, 1972–1974. J Infect Dis. 1976, 134: 286-289.View ArticlePubMedGoogle Scholar
- Patel JB, Wallace RJ, Brown-Elliott BA, Taylor T, Imperatrice C, Leonard DG, Wilson RW, Mann L, Jost KC, Nachamkin I: Sequence-based identification of aerobic actinomycetes. J Clin Microbiol. 2004, 42: 2530-2540. 10.1128/JCM.42.6.2530-2540.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Wallace RJ, Tsukamura M, Brown BA, Brown J, Steingrube VA, Zhang YS, Nash DR: Cefotaxime-resistant Nocardia asteroides strains are isolates of the controversial species Nocardia farcinica. J Clin Microbiol. 1990, 28: 2726-2732.PubMed CentralPubMedGoogle Scholar
- Wallace RJ, Brown BA, Tsukamura M, Brown JM, Onyi GO: Clinical and laboratory features of Nocardia nova. J Clin Microbiol. 1991, 29: 2407-2411.PubMed CentralPubMedGoogle Scholar
- Roth A, Andrees S, Kroppenstedt RM, Harmsen D, Mauch H: Phylogeny of the genus Nocardia based on reassessed 16S rRNA gene sequences reveals underspeciation and division of strains classified as Nocardia asteroides into three established species and two unnamed taxons. J Clin Microbiol. 2003, 41: 851-856. 10.1128/JCM.41.2.851-856.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Kageyama A, Torikoe K, Iwamoto M, Masuyama J, Shibuya Y, Okazaki H, Yazawa K, Minota S, Kroppenstedt RM, Mikami Y: Nocardia arthritidis sp. nov., a new pathogen isolated from a patient with rheumatoid arthritis in Japan. J Clin Microbiol. 2004, 42: 2366-2371. 10.1128/JCM.42.6.2366-2371.2004PubMed CentralView ArticlePubMedGoogle Scholar
- Lerner PI: Nocardiosis. Clin Infect Dis. 1996, 22: 891-905.View ArticlePubMedGoogle Scholar
- Moylett EH, Pacheco SE, Brown-Elliott BA, Perry TR, Buescher ES, Birmingham MC, Schentag JJ, Gimbel JF, Apodaca A, Schwartz MA, Rakita RM, Wallace RJ: Clinical experience with linezolid for the treatment of Nocardia infection. Clin Infect Dis. 2003, 36: 313-318. 10.1086/345907View ArticlePubMedGoogle Scholar
- Brown-Elliott BA, Ward SC, Crist CJ, Mann LB, Wilson RW, Wallace RJ: In vitro activities of linezolid against multiple Nocardia species. Antimicrob Agents Chemother. 2001, 45: 1295-1297. 10.1128/AAC.45.4.1295-1297.2001PubMed CentralView ArticlePubMedGoogle Scholar
- Geiseler PJ, Andersen BR: Results of therapy in systemic nocardiosis. Am J Med Sci. 1979, 278: 188-194.View ArticlePubMedGoogle Scholar
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