- Case report
- Open Access
Brachyspira pilosicoli bloodstream infections: Case report and review of the literature
© Bait-Merabet et al; licensee BioMed Central Ltd. 2008
- Received: 08 August 2008
- Accepted: 25 September 2008
- Published: 25 September 2008
Brachyspira pilosicoli is the etiologic agent of human and animal intestinal spirochetosis and is rarely implicated as a cause of bacteremia. Here, we describe the case of a B. pilosicoli spirochetemia in a 53-year-old male patient suffering from cardiogenic shock. This fastidious bacterium was isolated from blood, likely after translocation from the intestinal tract. Blood cultures were positive after 5 days of incubation (one day after the patient's death), highlighting the problem of the recovery of such type of fastidious bacterium. Identification was achieved by molecular methods (16S rRNA sequencing). A review of the English literature found only 8 cases of bacteremia caused by B. pilosicoli, mostly in immunocompromised or critically ill patients. Finally, difficulties in rapid and accurate diagnosis of B. pilosicoli bloodstream infections, in vitro antimicrobial susceptibility of human clinical isolates, and therapeutic options are discussed.
- Cardiogenic Shock
- Severe Underlying Disease
- Fastidious Bacterium
The genus Brachyspira, first described in 1982, belongs to the order of the Spirochaetales (like Treponema) and includes seven species to date: B. aalborgi, B. alvinipulli, B. hyodysenteriae, B. innocens, B. intermedia, B. murdochii, and B. pilosicoli http://www.bacterio.cict.fr/. Morphologically, they are coiled Gram-negative bacilli (2 to 11 μm long; 0.2 to 0.5 μm in diameter) and are motile due to the presence of flagella (8 to 30). These anaerobic intestinal spirochetes colonize the large intestine of humans and many animal species, and several species may cause diarrhea and other gastrointestinal diseases in these hosts . Although Brachyspira (formerly Serpulina) pilosicoli is the etiologic agent of porcine intestinal spirochetosis , B. pilosicoli and B. alborgi are both described in humans and are considered as agents of human intestinal spirochetosis (HIS) [3, 4]. In addition, B. pilosicoli was also isolated from patients with bacteremia, termed spirochetemia [5–7]. In this report, we describe a case of spirochetemia due to B. pilosicoli in a peculiar clinical context (cardiogenic shock) and review the literature.
A 53-year-old man, obese and smoker, was admitted to the cardiology unit of our hospital for shock associated with a third-degree atrioventricular block. Upon admission, five sets of peripheral blood cultures were systematically drawn; however, antibiotics were not initiated because the patient was afebrile. In the cardiology unit, he had two episodes of ventricular fibrillation treated by external cardioversion. Coronarography was performed and showed an occlusion of the right main coronary artery that disappeared spontaneously whereas the left main coronary artery was normal. Ventriculography was normal and aortography revealed the absence of aortic dissection. Due to persisting cardiogenic shock, the patient was then transferred to the medical intensive care unit on the same day where he received inotropic drugs. Because of leukocytosis (white blood cell count of 16,000 cells/mm3) and hypothermia (body temperature at 35.3°C), an antibiotic treatment with cefotaxime (3 g daily) was started. A transoesophageal echocardiography revealed right atrial and ventricular massive dilatation and an acute right ventricular dysfunction. A diagnosis of cardiogenic shock due to transient occlusion of the right main coronary artery was made. In the unit, his evolution was rapidly unfavourable with the occurrence of multiple and repeated episodes of ventricular fibrillation. The patient died four days later in a context of multiple organ failure associated with persistent cardiogenic shock and right ventricular failure.
The day after of the patient's death (i.e. five days of incubation), four out of five BacT/Alert® blood culture anaerobic bottles (bioMérieux, Marcy l'Etoile, France), yielded a Gram-negative spiral-shaped bacterium, suggesting a spirochete. After three days, the isolate was subcultured on 5% horse blood-containing agar plates under anaerobic conditions at 37°C and the growth appeared as a thin film with weak β-hemolysis. Phenotypically, all biochemical tests except hippurate were negative using the API 20 ANA and ID 32 ANA strips (bioMérieux). In addition, the isolate yielded a positive reaction for oxidase test and negative reactions for catalase and indole production tests.
Accurate identification of the clinical isolate was achieved by 16S rRNA sequence analysis, as previously described . The 16S rRNA nucleotide sequence of the clinical isolate was then compared to deposited sequences available from GenBank using the BLAST program. The sequence of the clinical isolate was 100% identical with those of B. pilosicoli ATCC 51139 and P43/6/78 strains (GenBank accession no. AY155458 and U14927, respectively).
Disk diffusion susceptibility testing was performed on Mueller-Hinton agar supplemented with 5% defibrinated horse blood according to the recommendations of the Antibiogram Committee of the French Society for Microbiology http://www.sfm.asso.fr/publi/general.php?pa=1. The strain was fully resistant to penicillin, amoxicillin, ticarcillin and piperacillin by producing a β-lactamase (cefinase test positive). It was also resistant to erythromycin, spiramycin, rifampin, vancomycin and colistin, and susceptible to amoxicillin/clavulanate, piperacillin/tazobactam, cefoxitin, cefotaxime, imipenem, clindamycin, pristinamycin, tetracyclin, chloramphenicol and metronidazole.
In this report, we describe a case of B. pilosicoli bloodstream infection in a context of cardiogenic shock. To the best of our knowledge, only a few cases of bacteremia caused by this species have been reported in the literature.
Characteristics of 9 confirmed cases of bacteremia caused by B. pilosicoli
No. of cases
Alcoholic/acute intoxication by ethylene glycol
Acute peritonitis (necrosis of caecum)
Tabagism, cardiogenic shock
Although spirochetemia remains very rare, incidence of B. pilosicoli bacteremia is probably more common than clinically appreciated since detection time in automated growth detection systems often exceed 5 days under anaerobic conditions . In addition, subculture of B. pilosicoli from blood culture media is also fastidious (generally from 3 to 5 days at 37°C) and phenotypic identification to the species-level cannot be achieved by using available commercial kits . By contrast, the use of genotypic identification by sequencing the 16S rRNA gene allows clear identification to the species-level. Therefore, the use of PCR assays using specific primers for the 16S rRNA gene to directly identify the spirochetes recovered in blood should be strongly recommended.
Even if the diagnosis is often delayed like here, preferred treatment of B. pilosicoli infections remains metronidazole. Indeed, all isolates of B. pilosicoli recovered from humans (n = 123) were susceptible to metronidazole . Although β-lactam antibiotics are spirocheticidal, our B. pilosicoli strain was resistant to penicillins due to production of a β-lactamase. A novel class D β-lactamase (OXA-63), recently identified in the chromosome of B. pilosicoli BM4442, may explain this phenotype . Unfortunately, prevalence of blaOXA-63 gene has not been studied; however, an in vitro antimicrobial susceptibility survey on 139 B. pilosocoli clinical isolates showed a 54.7% susceptibility rate to amoxicillin, suggesting that resistance to penicillins is common . As previously reported, our strain was also resistant to erythromycin, but susceptible to third-generation cephalosporins, carbapenems, tetracyclin and chloramphenicol, . The resistance to macrolides in B. pilosicoli is due to point mutations in positions 2058 and 2059 of the 23S rRNA gene .
Our report confirms that B. pilosicoli may cause bacteremia in immunocompromised and/or critically ill patients. The contribution of the spirochetemia to the lethal outcome of our patient remains unclear, since he was debilitated with an underlying poorly controlled cardiogenic shock, and received effective therapy. However, rapid and accurate diagnosis methods (e.g. PCR assays) should be employed to identify the etiologic microorganism and help adapt antibiotic therapy for such infections.
We did informed consent for the patient about publishing the manuscript of Annals of Clinical Microbiology and Antimicrobials. Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
- Hampson DJ, Oxberry SL, La T: Potential for zoonotic transmission of Brachyspira pilosicoli. Emerg Infect Dis. 2006, 12: 869-870.PubMed CentralView ArticlePubMedGoogle Scholar
- Trott DJ, Stanton TB, Jensen NS, Duhamel GE, Johnson JL, Hampson DJ: Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. Int J Syst Bacteriol. 1996, 46: 206-215.View ArticlePubMedGoogle Scholar
- Trivett-Moore NL, Gilbert GL, Law CL, Trott DJ, Hampson DJ: Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. J Clin Microbiol. 1998, 36: 261-265.PubMed CentralPubMedGoogle Scholar
- Mikosza AS, Hampson DJ: Human intestinal spirochetosis: Brachyspira aalborgi and/or Brachyspira pilosicoli?. Anim Health Res Rev. 2001, 2: 101-110.PubMedGoogle Scholar
- Fournie-Amazouz E, Baranton G, Carlier JP, Chambreuil G, Cohadon F, Collin P, Gougeon-Jolivet A, Hermès I, Lemarie C, Saint Girons I: Isolations of intestinal spirochaetes from the blood of human patients. J Hosp Infect. 1995, 30: 160-162. 10.1016/0195-6701(95)90159-0View ArticlePubMedGoogle Scholar
- Trott DJ, Jensen NS, Saint Girons I, Oxberry SL, Stanton TB, Lindquist D, Hampson DJ: Identification and characterization of Serpulina pilosicoli isolates recovered from the blood of critically ill patients. J Clin Microbiol. 1997, 35: 482-485.PubMed CentralPubMedGoogle Scholar
- Kanavaki S, Mantadakis E, Thomakos N, Pefanis A, Matsiota-Bernard P, Karabela S, Samonis G: Brachyspira (Serpulina) pilosicoli spirochetemia in an immunocompromised patient. Infection. 2002, 30: 175-177. 10.1007/s15010-002-2175-1View ArticlePubMedGoogle Scholar
- Dortet L, Legrand P, Soussy CJ, Cattoir V: Bacterial identification, clinical significance, and antimicrobial susceptibilities of Acinetobacter ursingii and Acinetobacter schindleri, two frequently misidentified opportunistic pathogens. J Clin Microbiol. 2006, 44: 4471-4478. 10.1128/JCM.01535-06PubMed CentralView ArticlePubMedGoogle Scholar
- Harland WA, Lee FD: Intestinal spirochaetosis. Br Med J. 1967, 3: 718-719.PubMed CentralView ArticlePubMedGoogle Scholar
- Calderaro A, Gorrini C, Peruzzi S, Piccolo G, Dettori G, Chezzi C: Occurrence of human intestinal spirochetosis in comparison with infections by other enteropathogenic agents in an area of the Northern Italy. Diagn Microbiol Infect Dis. 2007, 59: 157-163. 10.1016/j.diagmicrobio.2007.05.002View ArticlePubMedGoogle Scholar
- Tanahashi J, Daa T, Gamachi A, Kashima K, Kondoh Y, Yada N, Yokoyama S: Human intestinal spirochetosis in Japan; its incidence, clinicopathologic features, and genotypic identification. Mod Pathol. 2008, 21: 76-84.PubMedGoogle Scholar
- Barrett SP: Intestinal spirochaetes in a Gulf Arab population. Epidemiol Infect. 1990, 104: 261-266.PubMed CentralView ArticlePubMedGoogle Scholar
- Trott DJ, Mikosza AS, Combs BG, Oxberry SL, Hampson DJ: Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the eastern Highlands of Papua New Guinea. Int J Syst Bacteriol. 1998, 48 (Pt 3): 659-668.View ArticlePubMedGoogle Scholar
- Lee JL, Hampson DJ: Genetic characterisation of intestinal spirochaetes and their association with disease. J Med Microbiol. 1994, 40: 365-371.View ArticlePubMedGoogle Scholar
- Brooke CJ, Margawani KR, Pearson AK, Riley TV, Robertson ID, Hampson DJ: Evaluation of blood culture systems for detection of the intestinal spirochaete Brachyspira (Serpulina) pilosicoli in human blood. J Med Microbiol. 2000, 49: 1031-1036.View ArticlePubMedGoogle Scholar
- Brooke CJ, Hampson DJ, Riley TV: In vitro antimicrobial susceptibility of Brachyspira pilosicoli isolates from humans. Antimicrob Agents Chemother. 2003, 47: 2354-2357. 10.1128/AAC.47.7.2354-2357.2003PubMed CentralView ArticlePubMedGoogle Scholar
- Meziane-Cherif D, Lambert T, Dupechez M, Courvalin P, Galimand M: Genetic and biochemical characterization of OXA-63, a new class D β-lactamase from Brachyspira pilosicoli BM4442. Antimicrob Agents Chemother. 2008, 52: 1264-1268. 10.1128/AAC.00684-07PubMed CentralView ArticlePubMedGoogle Scholar
- Karlsson M, Fellstrom C, Johansson KE, Franklin A: Antimicrobial resistance in Brachyspira pilosicoli with special reference to point mutations in the 23S rRNA gene associated with macrolide and lincosamide resistance. Microb Drug Resist. 2004, 10: 204-208.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.