Open Access

Molecular epidemiological study of clinical Acinetobacter baumannii isolates: phenotype switching of antibiotic resistance

Annals of Clinical Microbiology and Antimicrobials201312:21

https://doi.org/10.1186/1476-0711-12-21

Received: 7 June 2013

Accepted: 11 August 2013

Published: 21 August 2013

Abstract

Background

The presence of clinical Acinetobacter baumannii (A. baumannii) isolates with differing antibiotic resistance phenotypes in the same patient causes difficulties and confusion in treatment. This phenomenon may be caused by reasons such as cross-infection from neighboring patients that switches to different A. baumannii strain, natural mutation of A. baumannii, inducing of different antibiotic resistance genes expression or acquisition of genes conferring resistance from another source. To elucidate this question, clinical A. baumannii strains, isolated from the same individual patients, showed antibiotic resistance phenotypes switching during the same hospitalization period, were attentively collected for further analysis. Molecular approaches for phylogenetic analysis, including pulsed field gel electrophoresis, multilocus sequence typing, and short tandem repeat analysis, were employed for the chronological studies.

Findings

Our results showed that antibiotic resistance phenotype switching could have occurred as a result through both cross-infection and natural mutation roots. Our results also suggest that rapid phenotype switching between paired isolates could occur during one single course of antibiotic treatment.

Conclusions

Though cross infection caused antibiotic resistance phenotype switching does occur, natural mutation of A. baumannii isolates is particularly cautious for antibiotic treatment.

Keywords

Acinetobacter baumannii Pulsed field gel electrophoresisMultilocus sequence typingShort tandem repeatPhenotype switch

Findings

Introduction

Acinetobacter baumannii (A. baumannii ) was identified from the environment in the early twentieth century, and has been isolated worldwide. The rapid spread of multidrug-resistant A. baumannii (MDRAB) in clinical settings has made choosing an appropriate antibiotic to treat these infections difficult for clinicians. A. baumannii within genetically uniform populations exhibit significant phenotypic variability [1]. For example, antibiotic susceptible clinical A. baumannii isolates can develop antibiotic resistant phenotypes, in a process called phenotype switching. Such phenotype switching can be perplexing for clinicians, in both interpreting microbiological results and choosing effective antibiotics.

Shanley et al. showed that Acinetobacter calcoaceticus can naturally uptake, incorporate, and stably maintain DNA in vitro[2]. Only a few reports have mentioned the rapid adaptation of A. baumannii isolates in a hospital environment [3, 4]. Determining whether the multiple resistance phenotype switching is due to cross-infection from neighboring patients or from natural mutation of the same A. baumannii isolate is important because of the different strategies needed to resolve the clinical issues. Here we report the rapid change of resistance phenotype of clinical A. baumannii isolates from individual patients during the same admission at a single medical institution in Taiwan.

Material and methods

Isolates and phenotyping

We designed a chronological study to collect pairs of phenotypically-identified A. baumannii isolates from individual patients during the same hospitalization period at Changhua Christian Hospital (CCH). Pool of samples for further analysis was collected from January 1 1998 to December 31 2008. Among those samples, there were three pairs of clinical A. baumannii isolates from CCH that met the inclusion criteria: Pair 1 (isolates 29-4 and 29-43, numbered according to their position in the CCH Bacterial Bank), Pair 2 (isolates 10-18 and 10-10), and Pair 3 (isolates 14-91 and 14-81). Phynotypic method to identify those A. baumannii isolates is using a Vitek-2 System (BioMerieux, Marcy l'Etoile, France). And, the isolates were identified according to 16S ribosomal RNA region at the molecular level, as previously described [5].

DNA isolation, ribotyping, and detection of short tandem repeats (STR) from clinical A. baumannii isolates

Genomic DNA was isolated from three colonies from an overnight culture grown on blood agar plates (bioMérieux, Den Bosch, The Netherlands) using a Bacterial Genomic DNA Isolation Kit III according to the manufacturer’s instructions (Roche, Mannheim, Germany). The ribotype pattern was interpreted to identify the group to which each strain belonged, as previously described [6]. The primer pair REP1R-I (5-IIIICGICGICATCIGGC-3) and REP2-I (5-ICGICTTATCIGGCCTAC-3) [7] was used to amplify putative REP-like elements from the bacterial DNA.

Pulsed field gel electrophoresis

We followed a standard protocol for pulsed-field gel electrophoresis (PFGE) analysis of the A. baumannii isolates. In brief, A. baumannii were plated on blood agar and incubated in a 5% CO2 atmosphere at 35°C for 16–24 h. Plug slices were digested with 20 U of Sgr AI. The DNA fragments were then separated in 1% Seakem Gold agarose gels (FMC BioProducts) at 14°C using a Bio-Rad CHEF DRIII PFGE system (Bio-Rad Laboratories, Hercules, CA, USA). Gels were run in 0.5× Tris-borate-EDTA (TBE; pH 8) at a 120° fixed angle and a fixed voltage (6 V/cm), with pulse intervals from 4–40 s for 20 h. Following staining and imaging, the chromosomal DNA restriction patterns produced by PFGE were interpreted using Tenover’s categorization [8].

Multilocus sequence typing

Multilocus sequence typing (MLST) was performed according to the method of Bartual et al. [9]. In brief, housekeeping genes for MLST were selected based on their sequence availability in GenBank, on prior studies of the phylogenetic relationships for the genus Acinetobacter, and on their use in MLST schemes for other bacterial species [1, 1012]. PCR primers were chosen from previous studies or were newly designed for amplification of the seven selected genes: citrate synthase (gltA), DNA gyrase subunit B (gyrB), glucose dehydrogenase B (gdhB), homologous recombination factor (recA), 60 kDa chaperonin (cpn60), glucose-6-phosphate isomerase (gpi), RNA polymerase 70 factor (rpoD). All PCR amplifications were performed in a MasterCycler gradient instrument (Eppendorf, Hamburg, Germany). Sequencing of internal fragments (~450 bp in size) of the selected housekeeping genes was performed in an ABI Prism 377 sequencer using the ABI Prism BigDye terminator cycle sequencing ready reaction kit v. 2 (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s recommendations.

Results

We collected clinical and microbiological profiles focusing on the three pairs of A. baumannii isolates from patients during an individual hospitalization. All three patients stayed at our institute for at least two weeks, and all of them received antibiotics following identification of the A. baumannii isolates (Table 1). The antibiotic susceptibility of clinical A. baumannii isolates are listed in Table 2. Four PFGE fingerprint patterns were detected in the three pairs of A. baumannii isolates in Figure 1. Furthermore, there appears to be a clear link of cross-infection between the PFGE types and the clinical data available for the isolates. Interpretation of the MLST data revealed that more than half of the MLST allelic profiles from the three pairs of A. baumannii isolates differed from those already in A. baumannii MLST databases (http://pubmlst.org/abaumannii/)[13]. Comparison of the sequence types (ST) of the three paired A. baumannii isolates showed similarity between the 29-4 and 29-43 A. baumannii isolates, especially in the allelic profiles of gltA, gdhB, recA, and rpoD (Tables 3, 4). However, there was a difference between the 14-91 and 14-81 paired isolates, especially in the allelic profiles of recA, cpn60, and rpoD (Tables 3, 4). These results indicated that isolates 29-4 and 29-43 are the same isolate, and that both paired 14-91 and 14-81 isolates and paired 10-18 and 10-10 isoaltes are different isolates (Tables 3, 4). The fingerprint patterns of the STRs were quite varied (Tables 3, 4). It is particularly interesting that rapid phenotype switching between the paired isolates (29-4 and 29-43) could occur during one course of antibiotic treatment.
Table 1

The time line of antimicrobial agents prescription

Event

Date

Patient number one, Pair 1

Date

Patient number two, Pair 2

Date

Patient number three, Pair 3

Admission day

July 29

Admission day

July 2

Admission day

June 3

Admission day

Antimicrobial agent

July 29 and Aug 4

Cefuroxime

July 2 and July 19

Ampicillin-sulbactam

June 3 and June 10

Ampicillin-sulbactam

Isolation day

Aug 2

Isolates 10-10 from sputum

July 19

Isolates 29-43 from sputum

June 7

Isolates 14-91 from sputum

Antimicrobial agent

Aug 4 and Aug 7

Ceftazidime

July 19 and July 26

Piperacillin-tazobactam

June 10 and June 17

Cefotaxime

Antimicrobial agent

Aug 7 and Aug 10

Piperacillin-tazobactam

July 26 and Aug 1

Levofloxacin

June 17 and June 24

Piperacillin-tazobactam

Antimicrobial agent

Since Aug 10

Imipenem-cilastatin

Since Aug 1

Imipenem-cilastatin

Since June 24

Meropenem

Isolation day

Aug 16

Isolates 10-10 from abscess

Aug 18

Isolates 29-4 from tip of central catheter

July 7

Isolates 14-81 from sputum

Table 2

Antibiotic susceptibility of clinical Acinetobacter baumannii isolates

Number of isolate

10-10

10-18

29-43

29-4

14-91

14-81

Date of isolation

2-Aug

16-Aug

19-Jul

18-Aug

9-Jun

7-Jul

Time of isolation

PM 02:38:55

PM 03:10:10

PM 04:21:03

PM 02:47:06

AM 09:12:47

AM 10:18:53

Diagnosis

pneumonia

Soft tissue infection

pneumonia

Catheter-related infection

pneumonia

pneumonia

Specimens

Sputum, tracheal aspirate (suction)

abscess

Sputum, tracheal aspirate (suction)

Tip of central catheter

Sputum, tracheal aspirate (suction)

Sputum, tracheal aspirate (suction)

Antibiotic

Minimum inhibitory

concentrations

(ug/mL)

   

AN

8

128

8

128

8

64

SAM

32

128

32

128

32

128

CTZ

8

64

8

64

8

32

LVF

2

128

2

128

2

64

IMP

2

16

2

16

2

16

PIP-TAZ

8

256

8

256

8

256

CRO

8

128

8

128

8

64

CFP

8

256

8

256

8

128

MEP

4

32

4

32

4

16

Notes: The susceptibility tests were performed using the Vitek-2 GN card (Biomerieux, Marcy l'Etoile, France). The results were interpreted using the CLSI breakpoints (Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility testing; Twenty-First Information Supplement. CLSI document M100-S21, CLSI, Wayne, PA; 2011).

AN: amikacin; SAM: ampicillin-sulbactam; CTZ: ceftazidime; LVF: levofloxacin; IMP: imipenem-cilastatin; PIP-TAZ: piperacillin-tazobactam; CRO: ceftriaxone; CFP:cefepime; MEP: meropenem; colistin and tigecycline and polymyxin B were not provided by Vitek-2 System.

Figure 1

PFGE fingerprints of three pairs of clinical A. baumannii isolates following digestion with the Sgr AI restriction enzyme.

Table 3

The results of pulsed field gel electrophoresis, multilocus sequence typing, and short tandem repeat analysis of three pairs of A. baumannii isolates

Isolates

Geno type(†)

PFGE type

ST Type(‡)

gltA

gyrB

gdhB

recA

cpn60

gpi

rpoD

STR type

10-18

Ab 1

A

this study (a1)

6

this study

this study

this study

this study

this study

this study

I

10-10

Ab 2

A

this study (a2)

11

this study

this study

this study

this study

this study

this study

II

29-4

Ab 3

B

this study (a3)

1

this study

3

2

2

this study

3

III

29-43

Ab 3

B

this study (a4)

1

3

3

2

this study

7

3

IX

14-91

Ab 4

C

this study (a5)

1

this study

this study

2

1

23

18

X

14-81

Ab 5

D

this study (a6)

1

this study

3

2

2

this study

3

XI

Notes

Sequences of amplified genes were compared with sequences from the A. baumannii MLST website (http://pubmlst.org/abaumannii/).

ST: sequence type.

† the given name of the genotype is defined as A. baumannii + number (Ab + number).

‡ the given name of the genotype is defined as Acinetobacter isolate + number (a + number).

Table 4

Aligmnent for three pairs of Acinetobacter baumannii siolates

10-10 VS 10-18

gltA

gyrB

gdhB

recA

cpn60

gpi

rpoD

Length

484

927

744

371

454

358

860

Score

684

1218

1258

667

819

662

1589

Identities

446/484

835/922

699/707

361/361

446/447

358/358

860/860

Difference

38

87

8

0

1

0

0

Gaps

0/484

4/922

4/707

0/361

1/447

0/358

0/860

29-4 VS 29-43

gltA

gyrB

gdhB

recA

cpn60

gpi

rpoD

Length

484

936

396

371

454

363

513

Score

894

806

732

686

778

464

948

Identities

484/484

450/457

396/396

371/371

421/421

265/272

513/513

Difference

0

7

0

0

0

7

0

Gaps

0

0

0

0

0

0

0

14-91 VS 14-81

gltA

gyrB

gdhB

recA

cpn60

gpi

rpoD

Length

484

932

396

371

421

360

513

Score

894

1701

399

686

773

392

931

Identities

484/484

924/925

318/369

371/371

420/421

253/273

510/513

Difference

0

1

51

0

1

20

3

Gaps

0

1

0

0

0

2

0

Discussion

The is the first report of phenotype switching of antibiotic resistance in clinical A. baumannii isolates in individual patients during the same hospitalization in Taiwan. While A. baumannii has been reported previously in Taiwan, and prolonged administration of broad-spectrum antibiotics will induce the development of antibiotic resistance in clinical A. baumannii isolates, little is known about the current clinical situation. It was demonstrated that a important evolutionary change of a single genotype was fundamental to the continuous rise observed in the number of A. baumannii infections [4].

The current study suggests that natural transformation and mutation of genotypes occurred in clinical A. baumannii isolates 29-43 and 29-4 on the basis of PFGE. We used three methods to determine the genetic similarity of the paired A. baumannii isolates: PFGE, MLST, and STR. Snelling et al. described a PCR assay using repetitive extragenic palindromic sequences to type A. calcoaceticus and A. baumannii strains [14], while Alcala et al. characterized a meningococcal epidemic wave using a MLST method [15], similar to that used in our study. The congruence between the MLST, PFGE, and STR data suggests that the findings of the current study are sound; however, further experiments are required to prove the relationships among the paired isolates.

In this study, we discovered natural mutation and rapid change of antibiotic resistance phenotype of clinical A. baumannii isolates from an individual patient. This is alarming as this particular clone seems to be able to effectively fill niches that were essentially uninhabited by A. baumannii in the past. Even in a relatively closed environment, the isolates of identical PFGE fingerprint patterns showed a variety of MLST patterns. Apparently, the MLST patterns of paired isolates 29-4 and 29-43 are capable of withstanding background mutation. It is possible that the mutation rate of this particular isolate may contribute to its success in coping with different environments.

Conclusions

This study provides novel insight into the clinical problem of whether different A. baumannii isolates from the same patient are due to cross-infection from neighboring patients or from natural mutation. This is important for clinicians because the treatments for the two causes are different. The approach for the first phenomenon is to enhance contact precautions in the clinical practice, whereas the second is the stepwise prescription of different antibiotics.

Availability of supporting data

None.

Ethical approval

Not required.

Abbreviations

A. baumannii: 

Acinetobacter baumannii

CCH: 

Changhua christian hospital

cpn60: 

60 kDa chaperonin

gdhB: 

Glucose dehydrogenase B

gltA: 

Citrate synthase

gpi: 

Glucose-6-phosphate isomerase

gyrB: 

DNA gyrase subunit B

MDRAB: 

Multidrug-resistant Acinetobacter baumannii

MLST: 

Multilocus sequence typing

PFGE: 

pulsed-field gel electrophoresis

recA: 

Homologous recombination factor

rpoD: 

RNA polymerase 70 factor

ST: 

Sequence types

STR: 

Short tandem repeats.

Declarations

Acknowledgements

Authors gratefully acknowledge the help of Dr. Chien-Shun Chiou at Center for Disease Control, Taiwan for invaluable assistance with the development of the array-based MLST system. Authors also thank Hsusan-Pei Lin, and Chialin Chang for technical assistance.

Authors’ Affiliations

(1)
Division of Infectious Diseases, Department of Internal Medicine, Changhua Christian Hospital
(2)
Department of Nursing, College of Medicine & Nursing, Hung Kuang University
(3)
Department of Life Science, College of Life Science, National Chung Hsing University

References

  1. Ko E, Yomo T, Urabe I: Dynamic clustering of bacterial population. Physica D. 1994, 75: 81-88. 10.1016/0167-2789(94)90276-3.View ArticleGoogle Scholar
  2. Shanley MS, Ahmadian-Tehrani M, Benjamin RC, Leher HF: Natural transformation in Acinetobacter calcoaceticus. SAAS Bull Biochem Biotechnol. 1990, 3: 27-31.PubMedGoogle Scholar
  3. Nwugo CC, Arivett BA, Zimbler DL, Gaddy JA, Richards AM, Actis LA: Effect of Ethanol on Differential Protein Production and Expression of Potential Virulence Functions in the Opportunistic Pathogen Acinetobacter baumannii. PLoS One. 2012, 7: e51936- 10.1371/journal.pone.0051936View ArticlePubMedPubMed CentralGoogle Scholar
  4. Zander E, Chmielarczyk A, Heczko P, Seifert H, Higgins PG: Conversion of OXA-66 into OXA-82 in clinical Acinetobacter baumannii isolates and association with altered carbapenem susceptibility. J Antimicrob Chemother. 2013, 68: 308-311. 10.1093/jac/dks382View ArticlePubMedGoogle Scholar
  5. Sahl JW, Johnson JK, Harris AD, Phillippy AM, Hsiao WW, Thom KA, Rasko DA: Genomic comparison of multi-drug resistant invasive and colonizing Acinetobacter baumannii isolated from diverse human body sites reveals genomic plasticity. BMC Genomics. 2011, 12: 291- 10.1186/1471-2164-12-291View ArticlePubMedPubMed CentralGoogle Scholar
  6. Gerner-Smidt P: Ribotyping of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex. J Clin Microbiol. 1992, 30: 2680-2685.PubMedPubMed CentralGoogle Scholar
  7. Versalovic J, Koeuth T, Lupski JR: Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991, 19: 6823-6831. 10.1093/nar/19.24.6823View ArticlePubMedPubMed CentralGoogle Scholar
  8. Turnover FC: Plasmid fingerprinting. A tool for bacterial strain identification and surveillance of nosocomial and community-acquired infections. Clin Lab Med. 1985, 5: 413-436.Google Scholar
  9. Bartual SG, Seifert H, Hippler C, Luzon MAD, Wisplinghoff H, Rodríguez-Valera F: Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii. J Clin Microbiol. 2005, 43: 4382-4390. 10.1128/JCM.43.9.4382-4390.2005View ArticlePubMedPubMed CentralGoogle Scholar
  10. Hülter N, Wackernagel W: Double illegitimate recombination events integrate DNA segments through two different mechanisms during natural transformation of Acinetobacter baylyi. Mol Microbiol. 2008, 67: 984-995. 10.1111/j.1365-2958.2007.06096.xView ArticlePubMedGoogle Scholar
  11. Kotetishvili M, Stine OC, Chen Y, Kreger A, Sulakvelidze A, Sozhamannan S, Morris JG: Multilocus sequence typing has better discriminatory ability for typing Vibrio cholerae than does pulsed-field gel electrophoresis and provides a measure of phylogenetic relatedness. J Clin Microbiol. 2003, 41: 2191-2196. 10.1128/JCM.41.5.2191-2196.2003View ArticlePubMedPubMed CentralGoogle Scholar
  12. Maiden MC, Bygraves JA, Feil E, Morelli G, Russell JE, Urwin R, Zhang Q, Zhou J, Zurth K, Caugant DA, Feavers IM, Achtman M, Spratt BG: Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci USA. 1998, 95: 3140-3145. 10.1073/pnas.95.6.3140View ArticlePubMedPubMed CentralGoogle Scholar
  13. Bartual SG, Seifert H, Hippler C, Luzon MA, Wisplinghoff H, Rodríguez-Valera F: Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. Clin Microbiol. 2005, 43: 4382-4390. 10.1128/JCM.43.9.4382-4390.2005.View ArticleGoogle Scholar
  14. Snelling AM, Gerner-smidt P, Hawkey PM, Heritage J, Parnell P, Porter C, Bodenham AR, Inglis T: Validation of Use of Whole-Cell Repetitive Extragenic Palindromic Sequence-Based PCR (REP-PCR) for Typing Strains Belonging to the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex and Application of the Method to the Investigation of a Hospital Outbreak. J Clinical Microbiology. 1996, 34: 1193-1202.Google Scholar
  15. Alcala B, Salcedo C, Arreaza L, Berro’n S, de la Fuente L, va’Zquezj JA: The epidemic wave of meningococcal disease in Spain in 1996–1997: probably a consequence of strain displacement. Med Microbiol. 2002, 51: 1102-1106.View ArticleGoogle Scholar

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© Chen and Huang; licensee BioMed Central Ltd. 2013

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