Primary extrahepatic alveolar echinococcosis of the lumbar spine and the psoas muscle
© Nell et al; licensee BioMed Central Ltd. 2011
Received: 9 November 2010
Accepted: 15 April 2011
Published: 15 April 2011
Alveolar echinococcosis (AE) of human being caused by Echinococcus multilocularis is a rare but important zoonosis especially in tempered zones of middle Europe and Northern America with endemic character in many countries. Due to the long incubation period, various clinical manifestations, critical prognosis, and outcome AE presents a serious and severe disease. The primary focus of infection is usually the liver. Although secondary affection of visceral organs is possible extrahepatic AE is highly uncommon. Moreover, the involvement of bone and muscle presents with an even lower incidence. In the literature numerous cases on hepatic AE have been reported. However, extrahepatic AE involving bones and/or muscles was described very rarely. We report a case of an 80-year-old man with primary extrahepatic alveolar Echinococcosis of the lumbar spine and the psoas muscle. The etiology, diagnosis, differential diagnoses, treatment options and outcome of this rare disease are discussed in context with the current literature.
Two types of Echinococcus species are known - Echinococcus granulosus (EG) and Echinococcus multilocularis (EM). EG causes cystic Echinococcosis (CE) while EM causes alveolar Echinococcosis (AE) [1–3]. AE is one of the most pathogenic zoonoses in the northern hemisphere with an annual incidence of 0.03 to 1.2 per 100.000 inhabitants [4, 5]. In Europe EM is endemic in Belgium, Luxembourg, France, Switzerland, Liechtenstein, Germany, Austria, Italy, Poland, and the Czech Republic. Latest epidemiology research on EM in Europe revealed additional endemic regions within urban and suburban areas. This seems to be due to increasing prevalence of EM in foxes (Vulpes vulpes), the primary host [4, 6]. Besides foxes, dogs and cats, also domestic pigs might be affected by EM .
The PNM classification of alveolar echinococcosis
Hepatic localization of the parasite
Primary tumor cannot be assessed
No detectable tumor in the liver
Peripheral lesions without proximal vascular and/or biliary involvement
Central lesions with proximal vascular and/or biliary involvement of one lobe (a)
Central lesions with hilar vascular or biliary involvement of both lobes and/or with involvement of two hepatic veins
Any liver lesion with extension along the vessels (b) and the biliary tree
Extra-hepatic involvement of neighboring organs [diaphragm, lung, pleura, pericardium, heart, gastric and duodenal wall, adrenal glands, peritoneum, retroperitoneum, parietal wall (muscles, skin, bone), pancreas, regional lymph nodes, liver ligaments, kidney]
No regional involvement
Regional involvement of contiguous organs or tissues
The absence or presence of distant Metastasis [lung, distant lymph nodes, spleen, CNS, orbita, bone, skin, muscle, kidney, distant peritoneum and retroperitoneum]
Not completely evaluated
No metastasis (c)
In this context we present the case of a primary extrahepatic AE, affecting the lumbar spine and the psoas muscle. We discuss the etiology, diagnosis, and therapy of this rare lesion based on the current literature.
An 80-year-old man presented with sudden onset of right upper and lower quadrant abdominal pain at an academic department for surgery in October 2009. In addition, he suffered from relapsing nausea and vomiting, also describing recurrent low-grade fever a few days before and subjective feeling of swelling of the right upper abdomen. Written informed consent was obtained from the patient for publication of this case report and accompanying images. Computed tomography (CT) of the abdomen was performed revealing several cystic lesions of different size in the right psoas muscle. At that time, abscess-like formations were considered to be the most likely diagnosis. Considering this the working diagnosis initial treatment included the intravenous administration of antibiotics - Piperacillin/Tazobactam - and the percutaneous drainage of the biggest abscess under CT guidance. A follow-up CT-scan showed a diminution of the drained abscess followed by clinical recovery. Therefore, another percutaneous drainage of another, smaller abscess was performed. This led to shrinkage of the lesion and further clinical recovery. The inflammatory parameters decreased and no microorganisms were detected in the drained fluid. Subsequently the patient was discharged home.
One week later, the patient presented with recurrent abdominal pain in the right lower quadrant at the same department he had initially turned to. An abdominal ultrasound showed several obvious abscesses in the right psoas muscle. After readmission, three CT-guided suction drainages were placed in the three biggest abscesses. The microbiological analysis of the drained liquid provided evidence of Staphylococcus aureus. Hence antibiotic treatment was restarted with Piperazillin/Tazobactam. Despite continuous drainage for 7 days a follow-up CT revealed size constancy of the abscesses. Therefore, surgery was indicated and an open debridement of the abscesses in the right psoas muscle with resection of pannus was performed. The intraoperative microbiological work-up proved Staph. epidermidis and Staph. warneri. Thus, the antibiotic treatment with Piperacillin/Tacobactam was continued with higher dose rate. The patient's general condition improved and he could be discharged to rehabilitation 22 days after readmission.
AE represents an important endemic parasitic zoonosis in the northern hemisphere . Owing to its late onset and various clinical features a certain delay until definite diagnosis is common and AE is often found incidentally.
In the present case the patient reported a long history of abdominal discomfort and pain with additional relapsing nausea, vomiting, fever, and a subjective feeling of swelling. The symptoms and clinical signs depend on the structures and/or organs affected. Therefore, the clinical presentation of AE is highly variable and not specific . In hepatic affection the symptoms include cholestatic jaundice and/or abdominal pain. Further symptoms are fatigue and weight loss .
X-ray and CT findings of osseous AE are unspecific. Inhomogeneous osteolysis and irregular bone destruction may be seen. In the soft tissues, CT can reveal cystic masses with irregular thickened septations . Magnetic resonance imaging (MRI) may show tube-like cavities/cystic lesions with a multi-vesicular morphology if soft tissue is infected. Diagnostic criteria for cystic lesions are high intensity on T2-weighted images and low intensity on T1-weighted images without contrast agent enhancement (see figure 2). Because of the contrast enhancing thickened margins and septations AE can easily be confused with abscess formations [15, 16].
In this context the multiple cyst-like formations in the right psoas muscle were initially considered as bacterial psoas abscesses (BIA). Since BIA are common in the elderly population, this diagnosis seemed to be corroborated at first . Moreover, antibiotic treatment and percutaneous drainage lead to a diminution of the abscess formation and improved general health status. Additionally the diagnosis of BIA was even more corroborated because of the growth of various Staph. species. According to Ricci et al. Staph. aureus is known as the most common microorganism with an incidence of over 88% in patients suffering from BIA [17, 18]. In parallel to AE BIA also cause unspecific clinical symptoms [17, 19–21]. The classic clinical triad of fever, back and limb pain is present in only 30% of the patients with BIA . Regarding etiology BIA is related with diabetes mellitus, intravenous drug abuse, AIDS, renal failure and immunosuppression [17, 22]. Vertebral osteomyelitis, septic arthritis or infectious sacroiliitis may also cause BIA [17, 22].
In the present case, the patient developed lumbar back pain. The CT examination of the lumbar spine was interpreted as vertebral osteomyelitis. Therefore, therapy included antibiotic medication and percutaneous drainage. These management strategies are recommended for treatment of BIA [6, 18–21, 23, 24]. Because of failure of the percutaneous drainage open surgery was performed in the further course.
The intraoperative aspect of tube-like cavities in the psoas muscle raised the suspicion for Echinococcosis.
Finally the presence of AE was confirmed by histopathology and laboratory setup. In addition to the radiological and macroscopic presentation the diagnosis of AE is based on serology and PCR . Histopathology typically reveals parasitic vesicles delineated by a Periodic-Acid-Schiff (PAS+) laminated layer [2, 10]. The periparasitic granuloma is composed of epitheloid cells lining the parasitic vesicles, macrophages, fibroblasts and myofibroblasts, giant multinucleated cells, and cells of the nonspecific immune response, surrounded by lymphocytes .
In the present case the postoperative serology was positive for AE. For serological diagnosis purified, recombinant or in-vitro produced E. multilocularis antigens are used, mainly Em2, Em2+ and Em18 (2). Em10 is an E. multilocularis specific recombinant antigen used for assays to confirm AE . According to the literature serology has a high sensitivity of 90 to 100% and a specificity of 95 to 100% . Carmena et al. described high sensitivity and specificity of serological tests ranging from 75 to 100% . The differentiation between E. multilocularis and E. granulosus is possible in 80 to 95% of cases using most of the purified antigens . Nevertheless serological interpretation can be difficult in disease with extrahepatic infestation and sometimes remains insufficient in differentiating AE from CE. Especially the use of total somatic antigens is considered to show a high degree of cross-reaction with other parasites . In contrast, Gottstein et al. described improved immunodiagnostic with Em2+ ELISA using purified species-specific antigens 9. The Western blot detects serum IgG in 97% of patients infected with Echinococcus and its sensitivity is higher than of the ELISA for the detection of Echinococcosis . However, it can distinguish E. multilocularis from E. granulosus in only 76% of cases.
In our patient, PCR was conducted additionally in tissue specimens resected from the psoas muscle and the lumbar vertebrae. The result was equal 100% for the presence of E. multilocularis. The aim of PCR is to detect specific nucleic acids of Echinococcus and consequently to differentiate E. multilocularis from E. granulosus. So PCR is increasingly accepted as a complementary diagnostic tool for Echinococcosis and has been used to confirm the pathology in unusual locations . Scheuring et al. were successful differentiating E. multilocularis and E. granulosus in a patient with osseous and subcutaneous locations . Georges et al. described two cases of extrahepatic osseous infestation where PCR was useful to diagnose AE because of limited serological interpretation .
Regarding medical history, vocational or part-time farming, gardening, forestry and hunting are known risk factors for a possible infection with AE . 559 patients were reported to the European Echinococcosis registry from 1982 to 2000 on a voluntary basis. 61.4% of 210 registered persons presented with risk factors as listed . Therapy of AE should be multidisciplinary addressing every potential organ affection. Early diagnosis is crucial for prognosis and outcome as mortality reaches up to 80% in untreated patients . In Europe therapy has changed average life expectancy at time of diagnosis from 3 years in the 1970s to 20 years in 2005 . Although the introduction of Benzimidazole in the early 1980s brought an eminent breakthrough in treatment of AE and CE, surgical therapy is mandatory . Whenever possible complete resection of AE lesions should be performed . Because of the infiltrating growth of parasitic tissue and the potential for metastases principles and rules of tumor surgery should be considered and followed. Unfortunately most patients are inoperable at the time of diagnosis . In the present case a subtotal resection of the right psoas muscle was performed due to the multitude of parasitic lesions and we removed the lumbar vertebrae 1 to 3 and filled them up with bone cement.
Although postoperative Benzimidazole therapy is mandatory in all patients, there is no commonly accepted guideline regarding pre-surgical drug therapy and duration of drug treatment. Some authors suggest that temporary treatment might be sufficient after complete resection . Reuter et al. recommend a Benzimidazole treatment for at least two years since residual parasitic tissue may remain undetected . In case of incomplete surgical resection and in inoperable patients long-term or even life-long Benzimidazole therapy is indicated . Recent studies clearly demonstrated a significant improvement of the 10-year survival rate in non-radically resected patients who received a long term Benzimidazole therapy in comparison to a historic control group . This is in line with the recommendation of Brunetti et al. suggesting lifelong Benzimidazole therapy in non-radically resected patients .
Follow-up of infected patients is crucial. This includes imaging in terms of CT and MRI at intervals of 2-3 years and ultrasound at shorter intervals, blood tests with determination of Albendazole blood levels and serological tests. Serological analysis in the follow-up presents a close relationship between clinical status and treatment of patients with AE although interpretation of serological tests in patients under Benzimidazole treatment but without complete resection is more complex . Scheuring et al. demonstrated that anti-Em2- and anti-Em18-antibodies rapidly decreased after complete resection of parasitic lesions and become undetectable .
In summary, primary extrahepatic AE of bone and muscle is an extremely rare disease. The clinical course leading to diagnosis can be long and difficult because of the various and unspecific clinical features. As in the case described psoas muscle abscesses are an important differential diagnosis. Recrudescent reappearance of obvious abscesses in a patient's history despite of the continuous administration of antibiotics and percutaneous drainage for several times has to direct one's attention to the possible presence of Echinococcosis. If a patient is suspected of having Echinococcosis imaging should be completed and serological tests and PCR should be done immediately. Early diagnosis is very important for prognosis and outcome and a multidisciplinary approach of treatment should be followed. Surgery and drug therapy are available options for treatment.
- Tappe D, Weise D, Ziegler U, Muller A, Mullges W, Stich A: Brain and lung metastasis of alveolar echinococcosis in a refugee from a hyperendemic area. J Med Microbiol. 2008, 57-Pt 11: 1420-3. 10.1099/jmm.0.2008/002816-0.View ArticleGoogle Scholar
- Brunetti E, Kern P, Vuitton DA: Writing Panel for the WHO-IWGE Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop. 2010, 114-1: 1-16. 10.1016/j.actatropica.2009.11.001.View ArticleGoogle Scholar
- Eckert J: Epidemiology of Echinococcus multilocularis and E. granulosus in central Europe. Parassitologia. 1997, 39-4: 337-44.Google Scholar
- Romig T: Echinococcus multilocularis in Europe--state of the art. Vet Res Commun. 2009, 33 (Suppl 1): 31-4. 10.1007/s11259-009-9244-1View ArticlePubMedGoogle Scholar
- Kern P, Bardonnet K, Renner E, Auer H, Pawlowski Z, Ammann RW, Vuitton DA: European echinococcosis registry: human alveolar echinococcosis, Europe, 1982-2000. Emerg Infect Dis. 2003, 9-3 (343-9):Google Scholar
- Deplazes P: Ecology and epidemiology of Echinococcus multilocularis in Europe. Parassitologia. 2006, 48-1-2: 37-9.Google Scholar
- Ammann RW, Eckert J: Cestodes. Echinococcus. Gastroenterol Clin North Am. 1996, 25-3: 655-89. 10.1016/S0889-8553(05)70268-5.View ArticleGoogle Scholar
- Kolligs FT, Gerbes AL, Durr EM, Schauer R, Kessler M, Jelinek T, Loscher T, Bilzer M: 52-year-old patient with subcutaneous space-occupying lesion in immunosuppression. Internist (Berl). 2003, 44-6: 740-5.Google Scholar
- Gottstein B, Hemphill A: Immunopathology of echinococcosis. Chem Immunol. 1997, 66: 177-208. full_textView ArticlePubMedGoogle Scholar
- Takakuwa M, Katsuki M, Matsuno T, Hirayama T, Yoshida E: Hydatid disease at the proximal end of the clavicle. J Orthop Sci. 2002, 7-4: 505-7. 10.1007/s007760200089.View ArticleGoogle Scholar
- Georges S, Villard O, Filisetti D, Mathis A, Marcellin L, Hansmann Y, Candolfi E: Usefulness of PCR analysis for diagnosis of alveolar echinococcosis with unusual localizations: two case studies. J Clin Microbiol. 2004, 42-12: 5954-6. 10.1128/JCM.42.12.5954-5956.2004.View ArticleGoogle Scholar
- Kellsey DC, Sproat HF: Echinococcus disease of bone; report of a case. J Bone Joint Surg Am. 1954, 36-A-6: 1241-8.Google Scholar
- Vuitton DA, Gottstein B: Echinococcus multilocularis and its intermediate host: a model of parasite-host interplay. J Biomed Biotechnol. 2010, 923 193-Google Scholar
- Turgut AT, Odev K, Kabaalioglu A, Bhatt S, Dogra VS: Multitechnique evaluation of renal hydatid disease. AJR Am J Roentgenol. 2009, 192-2: 462-7. 10.2214/AJR.08.1129.View ArticleGoogle Scholar
- Rubini-Campagna A, Kermarrec E, Laurent V, Regent D: Hepatic and extrahepatic alveolar echinococcosis: CT and MR imaging features. J Radiol. 2008, 89-6: 765-74. 10.1016/S0221-0363(08)73782-X.View ArticleGoogle Scholar
- Abdel Razek AA, El-Shamam O, Abdel Wahab N: Magnetic resonance appearance of cerebral cystic echinococcosis: World Health Organization (WHO) classification. Acta Radiol. 2009, 50-5: 549-54. 10.1080/02841850902878161.View ArticleGoogle Scholar
- Mallick IH, Thoufeeq MH, Rajendran TP: Iliopsoas abscesses. Postgrad Med J. 2004, 80-946: 459-62. 10.1136/pgmj.2003.017665.View ArticleGoogle Scholar
- Ricci MA, Rose FB, Meyer KK: Pyogenic psoas abscess: worldwide variations in etiology. World J Surg. 1986, 10-5: 834-43. 10.1007/BF01655254.View ArticleGoogle Scholar
- Penado S, Espina B, Francisco Campo J: Abscess of the psoas muscle. Description of a series of 23 cases. Enferm Infecc Microbiol Clin. 2001, 19-6: 257-60.View ArticleGoogle Scholar
- Charalampopoulos A, Macheras A, Charalabopoulos A, Fotiadis C, Charalabopoulos K: Iliopsoas abscesses: diagnostic, aetiologic and therapeutic approach in five patients with a literature review. Scand J Gastroenterol. 2009, 44-5: 594-9. 10.1080/00365520902745054.View ArticleGoogle Scholar
- Al-Hilli Z, Prichard RS, Roche-Nagle G, Deasy J, McNamara DA: Iliopsoas abscess: a re-emerging clinical entity not to be forgotten. Ir Med J. 2009, 102-2: 58-60.Google Scholar
- Santaella RO, Fishman EK, Lipsett PA: Primary vs secondary iliopsoas abscess. Presentation, microbiology, and treatment. Arch Surg. 1995, 130-12: 1309-13.View ArticleGoogle Scholar
- Lee YT, Lee CM, Su SC, Liu CP, Wang TE: Psoas abscess: a 10 year review. J Microbiol Immunol Infect. 1999, 32-1: 40-6.Google Scholar
- Lin MF, Lau YJ, Hu BS, Shi ZY, Lin YH: Pyogenic psoas abscess: analysis of 27 cases. J Microbiol Immunol Infect. 1999, 32-4: 261-8.Google Scholar
- Carmena D, Benito A, Eraso E: The immunodiagnosis of Echinococcus multilocularis infection. Clin Microbiol Infect. 2007, 13-5: 460-75. 10.1111/j.1469-0691.2007.01665.x.View ArticleGoogle Scholar
- Scheuring UJ, Seitz HM, Wellmann A, Hartlapp JH, Tappe D, Brehm K, Spengler U, Sauerbruch T, Rockstroh JK: Long-term benzimidazole treatment of alveolar echinococcosis with hematogenic subcutaneous and bone dissemination. Med Microbiol Immunol. 2003, 192-4: 193-5. 10.1007/s00430-002-0171-9.View ArticleGoogle Scholar
- Wilson JF, Rausch RL, Wilson FR: Alveolar hydatid disease. Review of the surgical experience in 42 cases of active disease among Alaskan Eskimos. Ann Surg. 1995, 221-3: 315-23. 10.1097/00000658-199503000-00015.View ArticleGoogle Scholar
- Vuitton DA: Benzimidazoles for the treatment of cystic and alveolar echinococcosis: what is the consensus?. Expert Rev Anti Infect Ther. 2009, 7-2: 145-9. 10.1586/14787188.8.131.52.View ArticleGoogle Scholar
- Tappe D, Gruner B, Kern P, Frosch M: Evaluation of a commercial Echinococcus Western Blot assay for serological follow-up of patients with alveolar echinococcosis. Clin Vaccine Immunol. 2008, 15-11: 1633-7. 10.1128/CVI.00272-08.View ArticleGoogle Scholar
- Reuter S, Jensen B, Buttenschoen K, Kratzer W, Kern P: Benzimidazoles in the treatment of alveolar echinococcosis: a comparative study and review of the literature. J Antimicrob Chemother. 2000, 46-3: 451-6. 10.1093/jac/46.3.451.View ArticleGoogle Scholar
- Torgerson PR, Schweiger A, Deplazes P, Pohar M, Reichen J, Ammann RW, Tarr PE, Halkik N, Mullhaupt B: Alveolar echinococcosis: from a deadly disease to a well-controlled infection. Relative survival and economic analysis in Switzerland over the last 35 years. J Hepatol. 2008, 49-1: 72-7. 10.1016/j.jhep.2008.03.023.View ArticleGoogle Scholar
- Tappe D, Frosch M, Sako Y, Itoh S, Gruner B, Reuter S, Nakao M, Ito A, Kern P: Close relationship between clinical regression and specific serology in the follow-up of patients with alveolar echinococcosis in different clinical stages. Am J Trop Med Hyg. 2009, 80-5: 792-7.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.