Table 1 Evaluating the virulence features in the selected MDR Pseudomonas aeruginosa strains treated with metabolites of halophilic bacteria or gamma irradiation
Selected Pseudomonas aeruginosa strains | Treatment | The change in the yield of virulence factors in P. aeruginosa strains after treatment compared to control (100%) | |||||
|---|---|---|---|---|---|---|---|
Biofilm (%) | Rhamnolipids (%) | Pyocyanin (%) | Pyoverdin (%) | Protease (%) | Hemolysins (%) | ||
P. aeruginosa U3 | Gamma irradiation (1 kGy) | 63.00 ± 2.83 | 47.24 ± 0.99 | – | – | 27.00 ± 1.41 | – |
Halomonas cupida (Halo-Rt1) | 81.00 ± 1.41 | 56.08 ± 5.66 | 14.92 ± 0.06 | 56.70 ± 1.24 | 3.00 ± 1.41 | 54.00 ± 0.01 | |
Halomonas elongate (Halo-Rt2) | 60.00 ± 0.02 | – | 37.97 ± 0.03 | 61.00 ± 0.91 | 32.1.41 | 61.00 ± 0.92 | |
Vigibacillus natechei (Halo-Rt3) | ND | ND | ND | ND | ND | ND | |
Sediminibacillus terrae (Halo-Rt4) | ND | ND | ND | ND | ND | ND | |
Halomonas almeriensis (Halo-Rt5) | ND | ND | ND | ND | ND | ND | |
P. aeruginosa NCR-RT1 | Gamma irradiation (1 kGy) | 26.00 ± 0.01 | 86.57 ± 0.28 | – | – | 25.00 ± 0.01 | – |
Halomonas cupida (Halo-Rt1) | 79.00 ± 5.66 | 32.06 ± 0.56 | 42.65 ± 0.08 | 86.00 ± 1.41 | 86.00 ± 0.01 | 50.00 ± 2.12 | |
Halomonas elongate (Halo-Rt2) | ND | ND | ND | ND | ND | ND | |
Vigibacillus natechei (Halo-Rt3) | 71.00 ± 0.02 | 32.06 ± 0.14 | 64.70 ± 0.04 | 90.00 ± 1.41 | 86.00 ± 1.41 | 43.00 ± 1.70 | |
Sediminibacillus terrae (Halo-Rt4) | ND | ND | ND | ND | ND | ND | |
Halomonas almeriensis (Halo-Rt5) | ND | ND | ND | ND | ND | ND | |
P. aeruginosa NCR-RT2 | Gamma irradiation (1 kGy) | 35.00 ± 1.41 | 118.15 ± 0.01 | – | – | 6.00 ± 1.41 | – |
Halomonas cupida (Halo-Rt1) | ND | ND | ND | ND | ND | ND | |
Halomonas elongate (Halo-Rt2) | 61.00 ± 1.41 | 41.38 ± 0.01 | – | 73.00 ± 1.4 | 9.00 ± 2.83 | – | |
Vigibacillus natechei (Halo-Rt3) | ND | ND | ND | ND | ND | ND | |
Sediminibacillus terrae (Halo-Rt4) | 30.00 ± 0.20 | – | 18.90 ± 0.04 | 83.00 ± 2.83 | 11.00 ± 0.01 | 75.00 ± 0.02 | |
Halomonas almeriensis (Halo-Rt5) | ND | ND | ND | ND | ND | ND | |
P. aeruginosa NCR-RT3 | Gamma irradiation (1 kGy) | 34.00 ± 0.01 | 206.40 ± 1.3 | – | – | 28.00 ± 0.01 | – |
Halomonas cupida (Halo-Rt1) | 81.00 ± 0.71 | 53.52 ± 0.01 | 152.50 ± 0.03 | 50.00 ± 0.01 | 2.00 ± 0.01 | 65.00 ± 2.12 | |
Halomonas elongate (Halo-Rt2) | ND | ND | ND | ND | ND | ND | |
Vigibacillus natechei (Halo-Rt3) | ND | ND | ND | ND | ND | ND | |
Sediminibacillus terrae (Halo-Rt4) | ND | ND | ND | ND | ND | ND | |
Halomonas almeriensis (Halo-Rt5) | 64.00 ± 0.01 | – | 33.35 ± 0.02 | 40.00 ± 1.41 | 7.00 ± 0.01 | 58.00 ± 0.01 | |