Fig. 3

Genomic Analysis of Plasmids Carrying tmexCD1-toprJ1. Gene extents and orientations are indicated by arrows labeled with gene names, with tmexCD1-toprJ1 genes and resistant genes highlighted in red. Insertion sequences (ISs) are shown in purple, hypothetical protein genes in green, and other genes in blue. Horizontal lines represent the plasmid backbone, while black boxes denote the genetic structure of the tmexCD1-toprJ1-carrying region and its circular intermediate. a Circular comparison between the tmexCD1-toprJ1-bearing plasmid pTGC-02-tmex and other plasmids with resembling backbones in the NCBI database. The outermost red circle denotes the reference plasmid pTGC-02-tmex. Comparison between the genetic context of tmexCD1-toprJ1 and those of closely related sequences, including pTGC-05-tmex, pLAP2_020097, pLAP2_020035, and P1. b Circular genetic mapping of tmexCD1-toprJ1-carrying plasmids, including pTGC-05-tmex, along with other similar plasmids, including pTGC-05-tmex, pKPT698-tmexCD (CP079784), pRGT40-1-tmexCD (CP075551), pSCKLB555-1(CP043933), pMH15-269M_1(AP023338), p18-29-MDR(MK262712), pHN111RT-1(MT647838), pRGF20-1-tmexCD (CP075455), and pKP32558-1(CP076031). c A linear comparative analysis was conducted to assess the genetic context of tmexCD1-toprJ1 in relation to closely related sequences, which included pHNAH8I-1 (accession: MK347425, a representative plasmid housing an RND efflux pump responsible for tigecycline resistance), K. pneumoniae pTGC-02-tmex (this study, accession CP132218), K. pneumoniae pTGC-05-tmex (this study, accession CP132221), pHNG11RT-1 (accession: MT637839), and pKPT698-tmexCD1 (accession: CP079784.1). Gene extents and orientations were represented by arrows labeled with gene names. The tmexCD1-toprJ1 genes and resistance-related genes were highlighted in red. ISs were indicated in purple, IS26 elements in green, conjugation transfer genes in yellow, and other genes in black. The plasmid backbone was symbolized by horizontal lines, and the genetic structure of the region carrying tmexCD1-toprJ1 and its circular intermediate was denoted by black boxes