Evaluating molecular epidemiology of carbapenem non-susceptible Klebsiella pneumoniae isolates with MLST, MALDI-TOF MS, PFGE

Table 2 Primers for virulence

Primers	Primer Sequence (5´→ 3´)	Amplicon Size (bp)	Tm (°C)	References
magA	F: GGTGCTCTTTACATCATTGC R: GCAATGGCCATTTGCGTTAG	1282	58	[13]
rmpA	F: ACTGGGCTACCTCTGCTTCA R: CTTGCATGAGCCATCTTTCA	535	50	[13]
kpn	F: GTATGACTCGGGGAAGATTA R: CAGAAGCAGCCACCACACG	626	55	[13]
allS	F: CCGAAACATTACGCACCTTT R: ATCACGAAGAGCCAGGTCAC	508	58	[13]
iutA	F:GGCTGGACATCATGGGAACTGG R: CGTCGGGAACGGGTAGAATCG	300	66	[13]
iucA	F: AATCAATGGCTATTCCCGCTG R: CGCTTCACTTCTTTCACTGACAGG	239	59	[12]
K1	F:GTAGGTATTGCAAGCCATGC R: GCCCAGGTTAATGAATCCGT	1047	55	[14]
K2	F: GACCCGATATTCATACTTGACAGAG R:CCTGAAGTAAAATCGTAAATAGATGGC	641	58	[3]
K5	F: TGGTAGTGATGCTCGCGA R: CCTGAACCCACCCCAATC	280	58	[3]
K20	F: CGGTGCTACAGTGCATCATT R: GTTATACGATGCTCAGTCGC	741	58	[3]
K54	F: CATTAGCTCAGTGGTTGGCT R: GCTTGACAAACACCATAGCAG	881	58	[3]
K57	F: CTCAGGGCTAGAAGTGTCAT R: CACTAACCCAGAAAGTCGAG	1037	58	[3]

ISSN: 1476-0711