Table 1 Oligonucleotide primers used for RT- qPCR in this study. mazEF, mqsRA, and higAB (TA system), algD (alginate), gyrB (housekeeping) in P. aeruginosa
TA system | \({\rm{Primers}}\,\left( {{\rm{5'}}\, \to \,{\rm{3'}}} \right)\) | Amplicon Size |
|---|---|---|
mazE | F: GACGATAGCCAGCTTTGTCG R: CCGCCTCGTTGATTTCTTCG | 77 |
mazF | F: CGCCCTAGCCATTCCTATCA R: TGCAGGGCTTCATCTATGGT | 192 |
mqsR | F: ACTGCATGGACGAAGAGGAT R: TCAGCTTCAAACCAGGGACT | 112 |
mqsA | F: CGCACAAAGCAAACACAACA R: TCTAGTGGCTCCGCTTTCAT | 181 |
higA | F: AGGGTACGAATCACGGCTAT R: AGATCATCTGCTGCTCAACG | 150 |
higB | F: AATATCCCTTAGCAATCGTGTTC R: TGAAAGATGCTGCGGAAA | 78 |
algD | F: GGCTATGTCGGTGCAGTATG R : GGCTATGTCGGTGCAGTATG | 72 |
gyrB | F: CTGCTTCACCAACAACATCC R: GGTGGCGATCTTGAACTTCT | 130 |