Scheme 2

Schematic diagram of results scored by the naked eye with color card. A To score the results via naked eye, a color card was designed as a tool for enabling accurate and objective diagnosis. In this card, six different patterns in the same color, were distributed in a row as indicated in the diagram. B The pattern was covered by tubes containing different shades yellow, from tube 1 to tube 5. If the pattern could be distinguished by the naked eye [e.g., as is the case with tubes 1, 2, and 3 in B, the tube containing the sample should be scored as negative, and the patient would not be categorized as having invasive fungal infections (IFI). If no pattern was observed (e.g., as is the case with tube 5), the tube containing the sample should be scored as positive, and the patient would be considered to have IFI. If the pattern could be distinguished faintly (e.g., tube 4), the tube containing sample should be scored as weakly positive, and the patient could potentially have IFI