Fig. 4

The presence of bacteriophage in the studied sample. A, B, and C: plaque morphology of vB_KshKPC-M. Phages were cultured on the Kp100 strain (Carbapenemase resistance K. pneumoniae), forming 4 mm plaques (4 ± 0.5 mm in diameter) with a clear center surrounded by a halo. D: titration of vB_KshKPC-M phage. E: Transmission electron microscope micrograph of negatively stained phage vB_KshKPC-M at 30,000 × magnification. vB_KshKPC-M has (A) a head diameter of approximately 50 nm (50 ± 5 nm) and (B) a flexible non-contractile tail approximately 145 nm long (145 ± 5 nm)