Table 4 Comparison of the CRISPR-GBS assay with several current GBS detection methods
| Â | RT-PCR [13] | RT-LAMP [12] | RT-RPA [11] | CRISPR-GBS |
|---|---|---|---|---|
LoD | 2–3 genome copies | 300 pg μL−1 | 98 genomic copies | 5 copies μL−1 |
Cross-reaction | No | No | No | No |
Turnaround time | Detection time of 35Â min (exclude DNA extraction) | Detection time of 60Â min (exclude DNA extraction) | Detection time of 30Â min (exclude DNA extraction) | Turnaround time of 35Â min (include DNA extraction) |
DNA extraction | DNA extraction kit (G NOME kit) | DNA extraction kit | DNA extraction kit (BD GeneOhmâ„¢ Strep B kit) | Heating lysis |
Target | cfb gene | fbs gene | cfb gene | cfb gene |
Temperature | Temperature cycling | A constant temperature of 63 ℃ | A constant temperature of 39 ℃ | A constant temperature of 37 ℃ |
Clinical samples | 15 vaginal swabs | None | 50 vaginal/anal samples | 179 vaginal/ cervical swabs |
Cost | Unknown | Unknown | Around $ 10 |