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Table 2 Details of the six discordant samples among CRISPR-GBS, qPCR, and culture-MS method

From: CRISPR/Cas12a-based assay for the rapid and high-sensitivity detection of Streptococcus agalactiae colonization in pregnant women with premature rupture of membrane

Sample no

Source

Individual test result

CRISPR-GBS

qPCRb (CT value)

Culture-MS result

42

cervix

Negative

Positive (23.11)

Positive

52

cervix

Negative

Undetected (0)

Positive

62

cervix

Negative

Undetected (0)

Positive

63

cervix

Negative

Negative (32.64)

Positive

75

cervix

784,806.94a

Negative (31.79)

Positive

85

cervix

Negative

Negative (26.59)

Positive

  1. aFluorescence signal value (a.u)
  2. bqPCR result was judged as positive when the cycle threshold (CT) value was > 0 and ≤ 25.89