Determination of critical concentration for drug susceptibility testing of Mycobacterium tuberculosis against para-aminosalicylic acid with clinical isolates with thyA, folC and dfrA mutations

Table 2 The molecular characteristics of 20 isolates carried mutations conferring PAS resistance

Locus	ID of isolates	Nucleotide changes	Amino acid changes	MIC (μg/ml)
thyA	22,757	G → T at 60	D20Y^a	> 32
	25,001	del_A at 115	Frameshift^a	> 32
	22,773	C → A at 225	H75N	> 32
	15,219	C → A at 225	H75N	> 32
	15,821	C → A at 225	H75N	> 32
	31,792	A → G at 259	T87A^a	> 32
	17170	A → G at 411	E137G^a	> 32
	30329	ins_C at 790	Frameshift^a	> 32
	28422	thyA gene deletion	–	> 32
	28198	thyA gene deletion	–	> 32
	25426	thyA gene deletion	–	> 32
	18402	thyA gene deletion	–	> 32
dfrA	29861	C → A at -70	Upstream control element^a	> 32
dfrA	26406	C → A at -70	Upstream control element^a	> 32
folC	15821	G → C at 146	R49P	> 32
	15765	A → G at 292	S98G	32
	18069	A → C at 431	K144T^a	8
	15010	A → G at 448	S150G	> 32
	25174	A → C at 458	E153A	4
	16003	A → C at 458	E153A	8
	14803	C → T at 1021	R341C^a	32

ISSN: 1476-0711