Curcumin and capsaicin regulate apoptosis and alleviate intestinal inflammation induced by Clostridioides difficile in vitro

Table 1 Oligonucleotide sequences used in this study

Target gene	Primer designation	Oligonucleotide sequence (5′-3′)	PCR conditions	Reference
BCL-2	BCL-2	F: GAGCTGGTGGTTGACTTTCTC	95 °C 10 min, 40 cycles (95 °C 10 s; 56 °C 35 s; 72 °C 20 s), 72 °C 5 min	[48]
BCL-2	BCL-2	R: TCCATCTCCGATTCAGTCCCT		[48]
TNF-α	TNF-α	F: AGCCCATGTTGTAGCAAACC		[49]
TNF-α	TNF-α	R: TGAGGTACAGGCCCTCTGAT		[49]
TGF-β	TGF-B	F: CAATTCCTGGCGATACCTCAG		[50]
TGF-β	TGF-B	R: GCACAACTCCGGTGACATCAA		[50]
NF-kB	NF-kB	F: CCAGACCAACAACAACCCCT		[51]
NF-kB	NF-kB	R: TCACTCGGCAGATCTTGAGC		[51]
SMAD3	SMAD3	F: GCCTGTGCTGGAACATCATC		[52]
SMAD3	SMAD3	R: TTGCCCTCATGTGTGCTCTT		[52]
β-actin	ACTBF	F: ATGTGGCCGAGGACTTTGATT		[53]
β-actin	ACTBF	R: AGTGGGGTGGCTTTTAGGATG		[53]

ISSN: 1476-0711