Fig. 5From: Evaluation the reactivity of a peptide-based monoclonal antibody derived from OmpA with drug resistant pulsotypes of Acinetobacter baumannii as a potential therapeutic approachWestern blot analysis of the mAb reactivity. OmpA protein is considered as the main nonspecific slow porin of A. baumannii. Since OmpA appears to be homologous with the E. coli OmpA and P. aeruginosa OprF, the bacterial lysates containing OmpA or similar protein were resolved in the 10% acrylamide gel and then exposed to the 3F10-C9 mAb to evaluate the antibody reactivity. Lanes 1–5 Lysates from pulsotypes A to E of A. baumannii, Lane 6 BSA-conjugated peptide, Lane 7 E. coli lysate, Lane 8 P. aeruginosa lysate, Lane 9 SeeBlue pre-stained protein standard MarkerBack to article page