Fig. 1
a Amplification curve of the optimal primer sets. b–d The sensitivity of recombinant plasmid AFM-1, a sputum-simulated sample, and a feces-simulated sample, respectively. Amplification curves were obtained by diluting recombinant plasmid AFM-1 DNA, sputum-simulated sample, and feces-simulated sample from 105 copies/reaction to 100 copies/reaction, respectively. Sterile distilled water was used as a negative control