Table 2 Biochemical and molecular identification of Burkholderia cepacia complex in hospital acquired infections
| Â | Methods | Target | Identification | Remarks |
|---|---|---|---|---|
Phenotypic methods | Conventional biochemical method | Catalase, Gluconate, Malate, Phenylacetate, leucine arylamidase activity | Overlapping biochemical profiles for Bcc, Ralstonia spp. and Pandoraea spp. | Bcc and non-Bcc cannot be distinguished |
Phoenix | Biochemicals—automated | Cannot identify Ralstonia pickettii | Misidentification rate for Bcc is 23% | |
VITEK 2 | Biochemicals—automated | Can identify Ralstonia pickettii (83%) | Misidentification rate for Bcc is 12% | |
Protein signature | VITEK MS | Mass spectrogram of the protein | Genus level identification of B. cepacia—55–63% R. pickettii identification—85–100% Pandoraea spp.—87% | Species within Bcc cannot be distinguished |
Bruker Biotyper | Mass spectrogram of the protein | Agreement between Bruker Biotyper and recA sequencing Genus level—100% Species level—76.9% B. cenocepacia—95.8–100% B. multivorans—78.5% B. contaminans—0% B. vietnamiensis—100% B. cepacia—30–33.3% | Can identify and discriminate Bcc from non-Burkholderia spp. Few species within Bcc cannot be distinguished | |
Molecular targets | recA | DNA recombinase enzyme for DNA repair | Promising for differentiation of Burkholderia species including Bcc | Non-Bcc cannot be distinguished |
hisA | Encodes for an enzyme involved in histidine biosynthesis | Could discriminate among the Bcc species | Non-Bcc cannot be distinguished | |
rspU | Coding for a ribosomal protein S21 | Burkholderia spp. and non-Burkholderia spp. can be distinguished | Species within Bcc cannot be distinguished | |
16S rRNA | Component of the 30S small subunit of a prokaryotic ribosome | Burkholderia spp. and non-Burkholderia spp. can be distinguished | Unacceptable for discrimination of Bcc intra-species |