Skip to main content

Table 3 PCR results for 26 CRAB isolates

From: Molecular characterization of β-lactamase genes in clinical isolates of carbapenem-resistant Acinetobacter baumannii

Isolate

Source

MIC

MLST Type

TEM

SHV

PER

KPC

VEB

CTX

MBL

EDTA DDS

ADC-7

ISaba1 + ADC-7

ampC

ISaba1 + ampC

OXA-23

OXA-24

OXA-40

OXA-51

ISaba1 + OXA-51

OXA-58

ICA1

int123

22

Sp

16

229

+

+

+

+

+

+

+

+

25

Sp

16

229

+

+

+

+

+

+

+

+

+

+

28

T

8

2

+

+

+

+

+

+

+

+

32

U

32

229

+

+

+

+

+

+

+

+

+

+

39

Sp

16

229

+

+

+

+

+

+

+

+

+

+

42

T

16

229

+

+

+

+

+

+

+

+

+

+

51

T

16

229

+

+

+

+

+

+

+

+

+

+

52

U

16

229

+

+

+

+

+

+

+

+

+

56

U

32

229

+

+

+

+

+

+

+

+

+

+

57

U

8

2

+

+

+

+

+

+

58

T

> 256

2

+

+

+

+

+

+

61

U

16

229

+

+

+

+

+

+

+

+

62

Sp

16

229

+

+

+

+

+

+

+

+

64

T

32

2

+

+

+

+

+

+

+

+

65

B

16

2

+

+

+

+

+

+

+

+

68

Sp

8

229

+

+

+

+

+

+

+

+

220

T

32

2

+

+

+

+

+

+

+

222

B

64

2

+

+

+

+

+

+

+

+

+

223

Sp

32

2

+

+

+

+

+

+

+

+

+

+

224

T

64

229

+

+

+

+

+

+

+

+

+

+

225

T

32

2

+

+

+

+

+

+

+

+

+

226

T

32

2

+

+

+

+

+

+

+

+

+

+

229

Sp

32

2

+

+

+

+

+

+

+

+

+

+

231

Sp

64

2

+

+

+

+

+

+

+

+

+

232

T

> 256

2

+

+

+

+

+

+

+

+

+

235

T

32

2

+

+

+

+

+

+

+

+

+

  1. Primer sets targeting β-lactamase genes (displayed by Ambler class), insertion sequence (ISAba1), and mobile gene elements. Source (Sp sputum, T tissue/wound, U urine, B blood); MIC meropenem (≤ 2 μg/mL = Sensitive, ≥ 8 μg/mL = Resistant); KPC (KPC subtypes 1, 2, 5, 10); CTX (CTX-M subtypes 1, 2 8, 9, 25); MBL metallo-β-lactamase (DIM, GIM, IMP, NDM, SIM, SPM, VIM); EDTA DDS double disk synergy assay, ICA1 class 1 integron cassette array, int123, conserved region of integrases 1, 2, and 3. All PCR reactions run in duplicate with proper control strains