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Table 3 PCR results for 26 CRAB isolates

From: Molecular characterization of β-lactamase genes in clinical isolates of carbapenem-resistant Acinetobacter baumannii

Isolate Source MIC MLST Type TEM SHV PER KPC VEB CTX MBL EDTA DDS ADC-7 ISaba1 + ADC-7 ampC ISaba1 + ampC OXA-23 OXA-24 OXA-40 OXA-51 ISaba1 + OXA-51 OXA-58 ICA1 int123
22 Sp 16 229 + + + + + + + +
25 Sp 16 229 + + + + + + + + + +
28 T 8 2 + + + + + + + +
32 U 32 229 + + + + + + + + + +
39 Sp 16 229 + + + + + + + + + +
42 T 16 229 + + + + + + + + + +
51 T 16 229 + + + + + + + + + +
52 U 16 229 + + + + + + + + +
56 U 32 229 + + + + + + + + + +
57 U 8 2 + + + + + +
58 T > 256 2 + + + + + +
61 U 16 229 + + + + + + + +
62 Sp 16 229 + + + + + + + +
64 T 32 2 + + + + + + + +
65 B 16 2 + + + + + + + +
68 Sp 8 229 + + + + + + + +
220 T 32 2 + + + + + + +
222 B 64 2 + + + + + + + + +
223 Sp 32 2 + + + + + + + + + +
224 T 64 229 + + + + + + + + + +
225 T 32 2 + + + + + + + + +
226 T 32 2 + + + + + + + + + +
229 Sp 32 2 + + + + + + + + + +
231 Sp 64 2 + + + + + + + + +
232 T > 256 2 + + + + + + + + +
235 T 32 2 + + + + + + + + +
  1. Primer sets targeting β-lactamase genes (displayed by Ambler class), insertion sequence (ISAba1), and mobile gene elements. Source (Sp sputum, T tissue/wound, U urine, B blood); MIC meropenem (≤ 2 μg/mL = Sensitive, ≥ 8 μg/mL = Resistant); KPC (KPC subtypes 1, 2, 5, 10); CTX (CTX-M subtypes 1, 2 8, 9, 25); MBL metallo-β-lactamase (DIM, GIM, IMP, NDM, SIM, SPM, VIM); EDTA DDS double disk synergy assay, ICA1 class 1 integron cassette array, int123, conserved region of integrases 1, 2, and 3. All PCR reactions run in duplicate with proper control strains