Fig. 1

mRNA expression analyses of the amplified fragments of mecA in CoNS with single SCCmec elements (a) and those with multiple SCCmec elements (b). Two-millilitres culture of CoNS treated with oxacillin (2 μg/ml) for 10 h were used for mecA mRNA transcription analyses by reverse transcription PCR (RT-PCR); after treatment with oxacillin (2 μg/ml) and actinomycin D (2 μg/ml), mecA mRNA half-lives were determined by RT-PCR (c). mRNA expression levels were described in terms of intensity using Quantity One Imager. Data are shown as the image of three independent experiments with similar results