Fig. 1

Detection of SCCmec types and virulence factor genes. a Multiplex PCR assay identifies SCCmec types and subtypes I, II, III, IVa, and V. Simultaneously the mecA gene was detected. The following DNA templates were used for PCR (by lane): M, marker; 1–5, Control strains (SCCmec type I–V); 6, S. aureus strains isolated from the volunteers in the study. b PCR assay identifies sea gene. The following DNA templates were used for PCR (by lane): M, marker; 9, positive control; 10–16, S. aureus strains isolated from the volunteers in the study. c Electrophoresis image of Pulsed field gel electrophoresis. The following DNA templates were used for electrophoresis (by lane): M, The positive control strain Salmonella ser. Braenderup standard strain H9812; 1–11, S. aureus strains isolated from the volunteers in the study. d PCR assay identifies seb gene. The following DNA templates were used for PCR (by lane): M, marker; 1, positive control; 2–8, S. aureus strains isolated from the volunteers in the study