Table 1 Oligonucleotides used to detect TEM mRNA and Enterobacteriaceae (as a positive hybridization control)
Probe name | Sequence (5′→3′) | Target | Detection purpose |
|---|---|---|---|
Enterobac-Alexa488 | TCGTGTTTGCACAGTGCTGTGTTT | 23S rRNA | Enterobacteriaceae (adapted with minor modifications from Bohnert et al. [23]) |
Enterobac-Komp | TCGTGTTTGCAGAGTGCTGTGTTT | 23S rRNA | Competitor for Enterobacteriaceae detection (adapted with minor modifications from Bohnert et al. [23]) |
Bla-TEM-CalFluor610 | GGAAATGTTGAATACTCAT AAAAGGGAATAAGGGCGAC CAGGAAGGCAAAATGCCGC GCGTTTCTGGGTGAGCAAA CAGCATCTTTTACTTTCAC CTCGTGCACCCAACTGATC GATCCAGTTCGATGTAACC CAAGGATCTTACCGCTGTT GTTCTTCGGGGCGAAAACT AAGTGCTCATCATTGGAAA CGCCACATAGCAGAACTTT CGTCAACACGGGATAATAC GACCGAGTTGCTCTTGCCC TCTGAGAATAGTGTATGCG GTGAGTACTCAACCAAGTC TAAGATGCTTTTCTGTGAC CTCTTACTGTCATGCCATC TTATGGCAGCACTGCATAA CCGCAGTGTTATCACTCAT TCGTTGTCAGAAGTAAGTT TTAGCTCCTTCGGTCCTCC CCATGTTGTGCAAAAAAGC CAAGGCGAGTTACATGATC TCAGCTCCGGTTCCCAACG CGTCGTTTGGTATGGCTTC CAGGCATCGTGGTGTCACG GCAACGTTGTTGCCATTGC GTTCGCCAGTTAATAGTTT GCCGGGAAGCTAGAGTAAG CCATCCAGTCTATTAATTG GTCCTGCAACTTTATCCGC GAAGGGCCGAGCGCAGAAG CAGCAATAAACCAGCCAGC GCTCACCGGCTCCAGATTT CAATGATACCGCGAGACCC TACCATCTGGCCCCAGTGC TAACTACGATACGGGAGGG CCTGACTCCCCGTCGTGTA TATTTCGTTCATCCATAGT CACCTATCTCAGCGATCTG ACCAATGCTTAATCAGTGA | mRNA | TEM β-lactamase mRNA |