Table 1 Primers used in this study for PCR and RT-PCR analyses
Gene | Sequence (5′–3′) | Fragment length (bp) |
|---|---|---|
intI | F:CCGAGGATGCGAACCACTTC R:CCGCCACTGCGCCGTTACCA | 789 |
traA | F:AAGTGTTCAGGGTGCTTCTGCGC R:GTCATGTACATGATGACCATTT | 272 |
traF | F:CGGTGATGATTTGCGAACGA R:AGCATTCCGGTCGGCCTGTA | 400 |
trbC | F:CGGYATWCCGSCSACRCTGCG R:GCCACCTGYSBGCAGTCMCC | 255 |
tnp513 | F:ATGTCGCTGGCAAGGAACGC R:GGGTTCGCTGCGAGGATTGT | 240 |
ISpa7 | F:TCAGGCCTTCATCGCTGCCATCAGG R:TAGGCGTACAGTGCTCTTTCAACGCA | 300 |
ISEcp1 | F:CTTCATTGGCATTGATAAGTTAG R:TGTAGCATCGGTTTCCCAGTTTC | 299 |
tnpU | F:CCAACTGATGGCGGTGCCTT R:CGGTATGGTGGCTTTCGC | 403 |
tnpA (Tn21) | F:ATGCCACGTCGTTCCATCCTGTCC R:CCGGGTCTGCTCCCGCTGGCC | 300 |
tnsA | F:GCAGCAGCCTTACAAGACGAG R:GCCACATAGCGCAACTCCTCC | 416 |
merA | F:GACCAGCCGCAGTTCGTCTA R:GCAGCASGAAAGCT GCTTCA | 462 |
mexA | F:CGACCAGGCCGTGAGCAAGCAGC R:GGAGACCTTCGCCGCGTTGTCGC | 275 |
oprD | F:ATGAAAGTGATGAAGTGGAGCG R:TTACAGGATCGACAGCGGATAG | 949 |
mexA in RT-PCR | F:GGCGACAACGCGGCGAAGG R:CCTTCTGCTTGACGCCTTCCTGC | 202 |
oprD in RT-PCR | F:CGGCGACATCAGCAACACC R:GGGCCGTTGAAGTCGGAGTA | 195 |
16S rRNA in RT-PCR | F:CCTACGGGAGGCAGCAG R:ATTACCGCGGCTGCTGG | 194 |