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Table 2 PCR conditions, primer sequences, and PCR products of icaA, icaD, and FnBPA genes

From: The potential of bacteriophage cocktail in eliminating Methicillin-resistant Staphylococcus aureus biofilms in terms of different extracellular matrices expressed by PIA, ciaA-D and FnBPA genes

The gene Primers sequences PCR product (bp) Reference Thermal cycles
icaA F-5′-GAC CTC GAA GTC AAT AGA GGT A R-5′-CCC AGT ATA ACG TTG GAT ACC 814 [28] 95 °C for 5 min, 35 cycles 94 °C 30 s, 59.5 °C 30 s, 72 °C 50 s, and 72 °C 5 min after conclusion of the 35 cycles [29]
icaD F-5′-AGG CAA TAT CCA ACG GTA A R-5′-GTC ACG ACC TTT CTT ATA TT 371
FnBPA F-5′-AGGGATCCGATGGTGGAGGTGGATA-3′ R-5′-AGCCCGGGTGGCGTTGGTGGCACGATTGGAG 1274 [30] 95 °C 5 min, 35 cycles 94 °C 30 s, 65.5 °C 30 s, 72 °C 1 min and 72 °C 5 min after conclusion of the 35 cycles [30]