Table 2 PCR conditions, primer sequences, and PCR products of icaA, icaD, and FnBPA genes
The gene | Primers sequences | PCR product (bp) | Reference | Thermal cycles |
|---|---|---|---|---|
icaA | F-5′-GAC CTC GAA GTC AAT AGA GGT A R-5′-CCC AGT ATA ACG TTG GAT ACC | 814 | [28] | 95 °C for 5 min, 35 cycles 94 °C 30 s, 59.5 °C 30 s, 72 °C 50 s, and 72 °C 5 min after conclusion of the 35 cycles [29] |
icaD | F-5′-AGG CAA TAT CCA ACG GTA A R-5′-GTC ACG ACC TTT CTT ATA TT | 371 | ||
FnBPA | F-5′-AGGGATCCGATGGTGGAGGTGGATA-3′ R-5′-AGCCCGGGTGGCGTTGGTGGCACGATTGGAG | 1274 | [30] | 95 °C 5 min, 35 cycles 94 °C 30 s, 65.5 °C 30 s, 72 °C 1 min and 72 °C 5 min after conclusion of the 35 cycles [30] |