|
Target microorganisms
|
Probes
|
Sequences (5′-3′)
|
Formamide content in hybridisation buffer (% v/v)
|
NaCl content in wash buffer (M)
|
Authors
|
|---|
|
C. albicans
|
Caal*
|
GCCAAGGCTTATACTCGCT
|
30
|
0.112
|
Kempf [10]
|
|
C. glabrata
|
Cagl*
|
CCG CCA AGC CAC AAG GAC T
|
30
|
0.112
|
Kempf [10]
|
|
C. parapsilosis
|
Capa*
|
CCTGGTTCGCCAAAAAGGC
|
20
|
0.225
|
Kempf [10]
|
|
Aspergillus spp.
|
Asp*
|
TGATACATTCCGAG
|
25
|
0.159
|
Wang [21]
|
|
C. neoformans and C. gattii
|
Cne205*
|
CCAGCCCTTATCCACCGA
|
20
|
0.225
|
Martins [14]
|
|
H. capsulatum
|
Hca1*
|
AGTCGAGGCTTTCAGCATGT
|
30
|
1.112
|
Silva Jr [22]
|
|
Fungi
|
Pan fungal**
|
CTCTGGCTTCACCCTATTC
|
30
|
0.112
|
Amann [23]
|
- *Oligonucleotide probe synthesised and directly 5′-labelled with the hydrophilic sulphoindocyanine fluorescent dye Cy3 (Thermo Hybaid, Ulm, Germany).
- **Oligonucleotide probe synthesised and directly 5′-labelled with fluorescein isothiocyanate (FITC) (Thermo Hybaid, Ulm, Germany).
