Evaluation of fluorescence in situ hybridisation (FISH) for the detection of fungi directly from blood cultures and cerebrospinal fluid from patients with suspected invasive mycoses

Table 1 Information regarding the probes used in the present study

Target microorganisms	Probes	Sequences (5′-3′)	Formamide content in hybridisation buffer (% v/v)	NaCl content in wash buffer (M)	Authors
C. albicans	Caal*	GCCAAGGCTTATACTCGCT	30	0.112	Kempf [10]
C. glabrata	Cagl*	CCG CCA AGC CAC AAG GAC T	30	0.112	Kempf [10]
C. parapsilosis	Capa*	CCTGGTTCGCCAAAAAGGC	20	0.225	Kempf [10]
Aspergillus spp.	Asp*	TGATACATTCCGAG	25	0.159	Wang [21]
C. neoformans and C. gattii	Cne205*	CCAGCCCTTATCCACCGA	20	0.225	Martins [14]
H. capsulatum	Hca1*	AGTCGAGGCTTTCAGCATGT	30	1.112	Silva Jr [22]
Fungi	Pan fungal**	CTCTGGCTTCACCCTATTC	30	0.112	Amann [23]

*Oligonucleotide probe synthesised and directly 5′-labelled with the hydrophilic sulphoindocyanine fluorescent dye Cy3 (Thermo Hybaid, Ulm, Germany).
**Oligonucleotide probe synthesised and directly 5′-labelled with fluorescein isothiocyanate (FITC) (Thermo Hybaid, Ulm, Germany).

ISSN: 1476-0711