Table 2 Purification steps of the enzyme chitinase from Bacillus subtilis TV-125A
Purification steps | Volume | Activity | Total activity | Protein amount | Specific activity | Purificationfold | |
|---|---|---|---|---|---|---|---|
(mL) | (EU/mL) | EU | % | (mg/mL) | (EU/mg) | ||
Crude extrat | 50 | 44.95 | 8155.0 | 100 | 6.46 | 6.95 | - |
Ammonium sulphate precipitation | 5 | 255.5 | 1277.5 | 15.7 | 1.64 | 155.79 | 22.4 |
(%0-20) | |||||||
DEAE Sephadex column | 20 | 55.2 | 1104.0 | 13.4 | 0.28 | 197.14 | 28.4 |