Table 1 Efficiency of blood PCR assays
From: Polymerase chain reaction–based assays for the diagnosis of human brucellosis
Primer | Sequence | Target gene | Sensitivity | Specificity | Positive predictive value | Negative predictive value | Reference |
|---|---|---|---|---|---|---|---|
B4 | TGGCTCGGTTGCCAATATCAA | bcsp31 | 100 | 100 | 100 | 98 | 14 |
B5 | CGCGCTTGCCTTTCAGGTCTG | ||||||
JPF | GCGCTCAGGCTGCCGACGCAA | omp2 | 98 | 100 | 100 | 96.1 | 14 |
JPR | ACCAGCCATTGCGGTCGGTA | ||||||
P1 | TGGAGGTCAGAAATGAAC | omp2 | 99 | 100 | 100 | 98 | 14 |
P2 | GAGTGCGAAACGAGCGC | ||||||
26A | GCCCCTGACATAACCCGCTT | bp26 | 98.5 | 100 | 100 | 97.1 | 14 |
26B | GAGCGTGACATTTGCCGATA | ||||||
F4 | TCGAGCGCCCGCAAGGGG | 16S | 53.1 | 100 | 53.1 | 100 | 35 |
R2 | AACCATAGTGTCTCCACTAA | rRNA |