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Table 5 Specificity of TaqMan PCR for detection of oprL and the AES-I HW2 locus

From: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa

   TaqMan assay
Sample a No of samples tested oprL HW2
   Ct TAMRA b Ct FAM b
AES-I sputum 1 31.61 21.33
Non-AES-I sputum 1 28.45 -
AES-I strains 31 23 - 30 18 - 30
Non-AES-I strains 8 28 - 30 -
  1. a All samples were purified using Whatman FTA Elute cards
  2. b Fluorescent dye used for primer labeling; see Materials and Methods.