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Table 5 Specificity of TaqMan PCR for detection of oprL and the AES-I HW2 locus

From: A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa

  

TaqMan assay

Sample a

No of samples tested

oprL

HW2

  

Ct TAMRA b

Ct FAM b

AES-I sputum

1

31.61

21.33

Non-AES-I sputum

1

28.45

-

AES-I strains

31

23 - 30

18 - 30

Non-AES-I strains

8

28 - 30

-

  1. a All samples were purified using Whatman FTA Elute cards
  2. b Fluorescent dye used for primer labeling; see Materials and Methods.
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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