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Table 1 Amplification master mix

From: Genotypic detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis strains by DNA sequencing: a randomized trial

Component

Volume/sample

Sterile nuclease free water

3 ul

Forward primer

6 ul

Reverse Primer

6 ul

Master mix

25 ul

DNA template

10 ul

Final volume

50 ul