Figure 3
Transmission electron micrographs of acapsular mutant Cap 67 grown in the presence of subfraction F2.4 from S. adstringens for 72 h, at 35°C. Untreated yeasts have a compact cell wall (cw), nucleus (n), nucleolus (nu), mitochondria (m) and many electron-dense granules (glycogen) (A). Yeasts treated with 1 μg/ml (B-D) and 2.5 μg/ml (E-F) showed drastic ultrastructural alterations. Disruption of cell wall (black arrow), membranous structures (black arrowhead and details in the box of Fig. E and F), vacuoles (v) and budding of vesicular bodies in the vacuole lumen (white asterisk) can be observed. Bars = 1 μm.