Table 1 Primers used for PCR amplification
| Â | Primer sequence (5' to 3') | Â | Reference | |
|---|---|---|---|---|
Target gene(s) | Forward | Reverse | Size of amplicon | Â |
blaTEM-1 | GCACGAGTGGGTTACATCGA | GGTCCTCCGATCGTTGTCAG | 310 | 14 |
blaPER-1 | ATGAATGTCATTATAAAAG | TTGGGCTTAGGGCAG | 927 | 14 |
blaIMP alleles1-21 | GTTTATGTTCATACWTCG | GGTTTAAYAAAACAACCAC | 432 | 14 |
blaVIM alleles | TTTGGTCGCATATCGCAACG | CCATTCAGCCAGATCGGCAT | 500 | 14 |
blaGIM | ATATTACTTGTAGCGTTGCCAGC | TTAATCAGCCGACGCTTCAG | 729 | 14 |
blaSHV | ATGCGTTATATTCGCCTGTG | TGCTTTGTTATTCGGGCCAA | 753 | 14 |
blaSIM | ATGAGAACTTTATTGATTTT | TTAATTAATGAGCGGCGGTT | 741 | This study |
blaCTX-M | ATGATGACTCAGAGCATTCGCCGCT | TCAGAAACCGTGGGTTACGATTTTCG | 876 | 14 |
blaADC | ATGCGATTTAAAAAAATTTCTTGT | TGGAATACGTTTATTGGTTAACATGA | 1081 | 14 |
OXA-23-like | TCTGGTTGTACGGTTCAGC | AGTCTTTCCAAAAATTTTG | 606 | 14 |
OXA-24-like | ATGAAAAAATTTATACTTCC | TTAAATGATTCCAAGATTTTC | 828 | 14 |
OXA-51-like | ACAGAARTATTTAAGTGGG | GGTCTACAKCCMWTCCCCA | 880 | 14 |
blaOXA-58 | ATGAAATTATTAAAAATATTGAGTTTAG | TTATAAATAATGAAAAACACCCAAC | 843 | 14 |
ISAba1 | ATGCAGCGCTTCTTTGCAGG | AATGATTGGTGACAATGAAG | 393 | 14 |
IS1133 | ATGACACATCTCAATGAGTTATAT | TTAACACGAATGCAGAAGTTGATG | 543 | 14 |
ISAb-F1/OXA/IS-R | AGTTGCACTTGGTCGAATGAA | CCATAGCTTTGTTGAGTTTGG | 540 | 22 |
ISAb-F2/OXA/IS-R | TTGAAAATACGCGCTTGACAGA | CCATAGCTTTGTTGAGTTTGG | 1104 | 22 |
ISAb-F3/OXA/IS-R | CTCTGTACACGACAAATTTCAC | CCATAGCTTTGTTGAGTTTGG | 1384 | 22 |
ISAba1-ampC-UP | GACCTGCAAAGAAGCGCTGCATA | TTGGTTCTTTTAAACCATATACC | 1502 | This study |
intI1 5'CS | GACGATGCGTGGAGACC | CTTGCTGCTTGGATGCC | 300 | 25 |
intI1 3'CS | ATCGCAATAGTTGGCGAAGT | GCAAGGCGGAAACCCGCC | 800 | 25 |
Variable region | GGCATCCAAGCAGCAAGC | AAGCAGACTTGACCTGAT | Variable | 25 |
intI2 | ACGATGCCTGCTTTTTGTACGGCTGC | CCGTCTATCCTGCTTGCACGATGCA | 962 | This study |
intI3 | TCAGCCGGGCGACAAGTGCAAGGCCA | ATGAACAGGTATAACAGAAAT | 1041 | This study |
aphA6 | ATGGAATTGCCCAATATTATTC | TCAATTCAATTCATCAAGTTTTA | 780 | 14 |
tet A | GCGCGATCTGGTTCACTCG | AGTCGACAGYRGCGCCGGC | 164 | 36 |
tet B | TACGTGAATTTATTGCTTCGG | ATACAGCATCCAAAGCGCAC | 206 | 36 |
qnrA1 to qnrA6 | AGAGGATTTCTCACGCCAGG | TGCCAGGCACAGATCTTGAC | 661 | 37 |
qnrB1 to qnrB6 | GGMATHGAAATTCGCCACTG | TTTGCYGYYCGCCAGTCGAA | 562 | 37 |
qnrS1 to qnrS2 | GCAAGTTCATTGAACAGGGT | TCTAAACCGTCGAGTTCGGCG | 605 | 37 |
gyrA (QRDR) | AAATCTGCCCGTGTCGTTGGT | GCCATACCTACGGCGATACC | 285 | 14 |
parC (QRDR) | AAAAATCAGCGCGTACAGTG | CGAGAGTTTGGCTTCGGTAT | 276 | 14 |
adeB | GGATTATGGCGACAGAAGGA | AATACTGCCGCCAATACCAG | 981 | This study |
adeR | ATGTTTGATCATTCTTTTTCTTTTG | TTAATTAACATTTGAAATATG | 687 | 14 |
adeS | TTCAACAAGAAGATTGGACC | CTTGCTCAATACGACGG | 114 | 28 |