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Table 1 Determination of neutralizer toxicity, material antiseptic activity and organism viability according to ASTM E 1054-02.

From: Pitfalls in efficacy testing – how important is the validation of neutralization of chlorhexidine digluconate?

Storage
time
Test organism exposed to
(name of the test)
Staphylococcus
aureus
Staphylococcus
epidermidis
Micrococcus
luteus
Corynebacterium
jeikeium
Candida
albicans
< 1 min neutralizer in saline peptone water
(neutralizer toxicity test)
1.86 ± 0.05 1.81 ± 0.03 1.77 ± 0.01 1.82 ± 0.02 2.05 ± 0.01
  antiseptic solution
(material control test)
0* 0* 0* 0* 0*
  saline peptone water
(organism viability test)
1.90 ± 0.05 1.84 ± 0.07 1.74 ± 0.03 1.79 ± 0.02 2.02 ± 0.03
3 h neutralizer in saline peptone water
(neutralizer toxicity test)
1.83 ± 0.02 1.81 ± 0.02 1.72 ± 0.03 1.89 ± 0.08 2.05 ± 0.06
  antiseptic solution
(material control test)
0* 0* 0* 0* 0*
  saline peptone water
(organism viability test)
1.88 ± 0.06 1.81 ± 0.02 1.73 ± 0.05 1.77 ± 0.08 2.03 ± 0.02
  1. Mean and SD of the number of CFU (log10) when exposed to the neutralizer in saline peptone water, the antiseptic solution or saline peptone water at storage times of < 1 min and 3 h; * significant difference (p < 0.05) in comparison to organism viability test, Tukey-HSD