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Table 1 Determination of neutralizer toxicity, material antiseptic activity and organism viability according to ASTM E 1054-02.

From: Pitfalls in efficacy testing – how important is the validation of neutralization of chlorhexidine digluconate?

Storage

time

Test organism exposed to

(name of the test)

Staphylococcus

aureus

Staphylococcus

epidermidis

Micrococcus

luteus

Corynebacterium

jeikeium

Candida

albicans

< 1 min

neutralizer in saline peptone water

(neutralizer toxicity test)

1.86 ± 0.05

1.81 ± 0.03

1.77 ± 0.01

1.82 ± 0.02

2.05 ± 0.01

 

antiseptic solution

(material control test)

0*

0*

0*

0*

0*

 

saline peptone water

(organism viability test)

1.90 ± 0.05

1.84 ± 0.07

1.74 ± 0.03

1.79 ± 0.02

2.02 ± 0.03

3 h

neutralizer in saline peptone water

(neutralizer toxicity test)

1.83 ± 0.02

1.81 ± 0.02

1.72 ± 0.03

1.89 ± 0.08

2.05 ± 0.06

 

antiseptic solution

(material control test)

0*

0*

0*

0*

0*

 

saline peptone water

(organism viability test)

1.88 ± 0.06

1.81 ± 0.02

1.73 ± 0.05

1.77 ± 0.08

2.03 ± 0.02

  1. Mean and SD of the number of CFU (log10) when exposed to the neutralizer in saline peptone water, the antiseptic solution or saline peptone water at storage times of < 1 min and 3 h; * significant difference (p < 0.05) in comparison to organism viability test, Tukey-HSD
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Annals of Clinical Microbiology and Antimicrobials

ISSN: 1476-0711

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