Table 2 Details of gene loci and the corresponding primer sequences used for MLST analysis
Gene | Locus | Gene size (bp) | Co-ordinates | PCR product size (bp) | Size of polymorphic sequence (bp) | Function | Primer sequences |
|---|---|---|---|---|---|---|---|
adk | LIC12852 | 564 | 3458298–3458861 | 531 | 430 | Adenylate Kinase | F-GGGCTGGAAAAGGTACACAA |
R-ACGCAAGCTCCTTTTGAATC | |||||||
icdA | LIC13244 | 1197 | 3979829–3981025 | 674 | 557 | Isocitarate Dehydrogenase | F-GGGACGAGATGACCAGGAT |
R-TTTTTTGAGATCCGCAGCTTT | |||||||
LipL41 | LIC12966 | 1068 | 3603575–3604642 | 520 | 518 | Outermenbrane Lipoprotein LipL41 | F-TAGGAAATTGCGCAGCTACA |
R-GCATCGAGAGGAATTAACATCA | |||||||
rrs2 | LIC11508 | 1512 | 1862433–1863944 | 541 | 452 | 16S ribosomal RNA | F-CATGCAAGTCAAGCGGAGTA |
R-AGTTGAGCCCGCAGTTTTC | |||||||
secY | LIC12853 | 1383 | 3458869–3460251 | 549 | 549 | Translocase pre-protein secY | F-ATGCCGATCATTTTTGCTTC |
R-CCGTCCCTTAATTTTAGACTTCTTC | |||||||
LipL32 | LIC11352 | 819 | 1666299–1667117 | 474 | 474 | Outermenbrane Lipoprotein LipL32 | F-ATCTCCGTTGCACTCTTTGC |
R-ACCATCATCATCATCGTCCA |