An indirect enzyme-linked immunosorbent assay for rapid and quantitative assessment of Type III virulence phenotypes of Pseudomonas aeruginosa isolates

Table 1 Reference bacterial strains used in this study

Strain	Relevant characteristic(s)^a	Reference(s) or source
PA103	Virulent lung isolate of P. aeruginosa; known type III secreted effector proteins are ExoT and ExoU	[45, 46]
PAO1	Reference P. aeruginosa strain; known type III secreted effector proteins are ExoS, ExoT and ExoY	[47, 48]
mutN	PA103pscJ::Tn5Gm^R; defective in Type III secretion	[39]
PA103ΔU	PA103 with an in-frame deletion of amino acids (aa) 330–571 of ExoU	[35]
PA103ΔT	PA103 with a xylE aacC1 cassette replacing aa 36–348 of exoT; Gm^R	[35]
PA103ΔUΔT	PA103ΔU with a xylE aacC1 cassette replacing aa 36–348 of exoT; Gm^R	[35]
PA103ΔpcrV	PA103 with an in-frame deletion of aa 10–277 of PcrV	(L. Li, L. Mijares, and B. I. Kazmierczak, submitted for publication)
PA103ΔpopD	PA103 with an in-frame deletion of aa 7–274 of PopD	(L. Li, L. Mijares, and B. I. Kazmierczak, submitted for publication)
PA103ΔpopB	PA103 with an in-frame deletion of aa 11–371 of PopB	(L. Li, L. Mijares, and B. I. Kazmierczak, submitted for publication)

ISSN: 1476-0711