Figure 2

The effect of prolonged (24 h) exposure to amphotericin B (0.5 μg ml⁻¹or 1.0 μg ml⁻¹) on wild-type and selected deletion mutants of S. cerevisiae measured by the production of reactive oxygen species (ROS) within yeast cells. Data presented are ratios of the number of fluorescent cells compared to the total population; at least 200 cells from ten fields of view were used to analyse each treatment. Incubation with amphotericin B induced significantly greater production of ROS in treated wild type BY4742, Δaif1 and Δstm1. Amphotericin B had no significant effect on Δizh2 and Δizh3. The data shown are means and standard deviations from three replicate experiments and were analysed by the Manny-Whitney test to compare treated samples with the untreated control; *p < 0.05, **p < 0.01, ***p < 0.001.