Figure 1

The effects of exposure to polyene antifungals on the wild-type and selected deletion mutants of Saccharomyces cerevisiae . The effect of drug was determined by expressing each treatment as a percentage of control growth to account for variations in starting cell numbers between treatments and experiments. (a-b) Saccharomyces cerevisiae was exposed to two concentrations of amphotericin B; 1.0 μg ml⁻¹ and 2.0 μg ml⁻¹ for one, two and three hours. The number of viable cells reduced over time for each strain, however Δizh2 and Δizh3 were significantly more resistant than the wild-type and other strains tested at all doses. (c-d) S. cerevisiae was exposed to two concentrations of nystatin; 2.5 μg ml⁻¹ and 5.0 μg ml⁻¹ for one, two and three hours. The number of viable cells reduced over time for each strain; however Δizh2 and Δizh3 were significantly more resistant than the other strains after two and three hour’s incubation. The data in a-d are means and standard deviations of three replicate experiments: these were analysed by repeated measures ANOVA and Bonferroni’s multiple comparison test; *p < 0.05, **p < 0.01, ***p < 0.001.