Uropathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties

Momtaz, Hassan; Karimian, Azam; Madani, Mahboobeh; Safarpoor Dehkordi, Farhad; Ranjbar, Reza; Sarshar, Meysam; Souod, Negar

doi:10.1186/1476-0711-12-8

Annals of Clinical Microbiology and Antimicrobials

Table 1 Primers used for detection of virulence genes in uropathogenic Escherichia coli

From: Uropathogenic Escherichia coli in Iran: Serogroup distributions, virulence factors and antimicrobial resistance properties

Gene	Primer name	Primer sequence (5'-3')	Size of product (bp)	Reference
*set-1*	set-1-F	GTGAACCTGCTGCCGATATC	147	[21]
	set-1-R	ATTTGTGGATAAAAATGACG
*sen*	Sen-F	ATGTGCCTGCTATTATTTAT	799	[21]
	Sen-R	CATAATAATAAGCGGTCAGC
*astA*	astA-F	ATGCCATCAACACAGTATAT	110	[21]
	astA-R	GCGAGTGACGGCTTTGTAGT
*sigA*	sigA-F	TCCTCGGTATTATTTTATCC	408	[21]
	sigA-R	CGTAACCCCTGTTGTTTCCAC
*sap*	Sap_f	TACCCTCCACAACAGAGAATG	832	[21]
	Sap-R	TACCCTCCACAACAGAGAATG
*pic*	Pic-F	ACTGGATCTTAAGGCTCAGGAT	500	[21]
	Pic-R	GACTTAATGTCACTGTTCAGCG
*pap*	pap3	GCAACAGCAACGCTGGTTGCATCAT	336	[27]
	pap4	AGAGAGAGCCACTCTTATACGGACA
*cnf1*	cnf1	AAGATGGAGTTTCCTATGCAGGAG	498	[27]
	cnf2	TGGAGTTTCCTATGCAGGAG
*hlyA*	hly1	AACAAGGATAAGCACTGTTCTGGCT	1177	[27]
	hly2	ACCATATAAGCGGTCATTCCCGTCA
*sfa*	sfa1	CTCCGGAGAACTGGGTGCATCTTAC	410	[28]
	sfa2	CGGAGGAGTAATTACAAACCTGGCA
*afa*	afa1	GCTGGGCAGCAAACTGATAACTCTC	750	[28]
	afa2	CATCAAGCTGTTTGTTCGTCCGCCG
*iuc*	iuc1	ATGAGAATCATTATTGACATAATTG	1482	[29]
	iuc2	CTCACGGGTGAAAATATTTT
*fim*	fim1	GAGAAGAGGTTTGATTTAACTTATTG	559	[30]
	fim2	AGAGCCGCTGTAGAACTGAGG
*papGI*	papGJ96-F	TCGTGCTGAGGTCCGGAATTT	461	[31]
	papGJ96-R	TGGCATCCCCCAACATTATCG
*papGII*	papGIA2-F	GGGATGAGCGGGCCTTTGAT	190	[31]
	papGIA2-R	CGGGCCCCCAAGTAACTCG
*papGIII*	prsJ96-F	GGCCTGCAATGGATTTACCTGG	258	[31]
	prsJ96-R	CCACCAAATGACCATGCCAGAC
*kpsMT*	kpsM481F	CCATCGATACGATCATTGCACG	400	[32]
	kpsM481R	ATTGCAAGGTAGTTCAGACTCA
*iha*	IHA-F	CTGGCGGAGGCTCTGAGATCA	827	[23]
	IHA-R	TCCTTAAGCTCCCGCGGCTGA
*iron*	IRONEC-F	AAGTCAAAGCAGGGGTTGCCCG	665	[23]
	IRONEC-R	GACGCCGACATTAAGACGCAG
*ompT*	ompT-F	ATCTAGCCGAAGAAGGAGGC	559	[33]
	ompT-R	CCCGGGTCATAGTGTTCATC
*usp*	usp -F	ACATTCACGGCAAGCCTCAG	440	[22]
	usp -R	AGCGAGTTCCTGGTGAAAGC
*iss*	Iss-F	ATCACATAGGATTCTGCCG	309	[34]
	Iss-R	CAGCGGAGTATAGATGCCA
*irp2*	Irp2-F	AAGGATTCGCTGTTACCGGAC	413	[34]
	Irp2-R	AACTCCTGATACAGGTGGC
*tsh*	Tsh-F	ACTATTCTCTGCAGGAAGTC	824	[34]
	Tsh-R	CTTCCGATGTTCTGAACGT
*vat*	Vat-F	TCCTGGGACATAATGGTCAG	981	[34]
	Vat-R	GTGTCAGAACGGAATTGT
*cva*	Cva-F	TGGTAGAATGTGCCAGAGCAAG	1181	[34]
	Cva-R	GAGCTGTTTGTAGCGAAGCC

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ISSN: 1476-0711

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