Table 1 Time to Identification of isolates from blood and peritoneal fluids by culture and PNA-FISH
BLOOD CULTURES | |||||
|---|---|---|---|---|---|
Organism | N | Mean Culture Hours to ID (range) | Mean PNA-FISH Hours to ID (range) | Mean Hours Saved with PNA-FISH (range) | P(Time Saved) |
All Gram Positive | 54 | 78.5 h | 8.3 h | 70.2 h | <0.0001 |
(20–112) | (3–22) | (1 – 94) | |||
CoNS | 34 | 72.2 | 12.5 h | 59.7 h | <0.0001 |
(20–103) | (3 – 22) | (1 – 93) | |||
S. aureus | 10 | 79.0 h | 9.9 h | 65.8 h | <0.0001 |
(47 – 98) | (3–20) | (33 – 93) | |||
E. faecalis | 5 | 73.8 h | 5.8 h | 68.0 h | <0.0001 |
(60 – 97) | (3 – 15) | (45 – 94) | |||
Other Enterococci (includes E. faecium) | 5 | 89.0 h | 6.4 h | 82.6 h | <0.0001 |
(70 –112) | (3 – 20) | (69 – 93) | |||
All Gram Negative | 19 | 76.2 h | 12.1 h | 64.1 h | 0.0002 |
(23 –112) | (3 – 24) | (3–115) | |||
E. coli | 9 | 78.4 h | 9.2 h | 69.4 h | <0.0001 |
(25 –108) | (5 – 18) | (20 – 91) | |||
K. pneumoniae | 5 | 71.6 h | 17.8 h | 54.0 h | 0.0109 |
(23 –112) | (5 – 24) | (3 – 91) | |||
P. aeruginosa | 5 | 70.0 h | 7.8 h | 58.8 h | 0.0002 |
(40–94) | (3 – 22) | (18 – 90) | |||
All Candida | 4 | 96.0 h | 13.2 h | 83.0 h | 0.0095 |
(72 –130) | (7.2 – 17.2) | (65 – 115) | |||
C. albicans | 2* | 72.0 h | 7.2 h | 64.8 h | N/A |
C. glabrata | 1 | 130 h | 15.2 h | 114.8 h | N/A |
C. parapsilosis | 1 | 86 | 17.2 h | 68.8 h | NA |
PERITONEAL FLUID CULTURES † | |||||
All Gram Positive | 5 | 73.2 h | 10.6 h | 62.6 h | 0.0070 |
(23–111) | (2 – 22.2) | (21 – 90.8) | |||
CoNS | 1 | 111.0 h | 19.6 h | 91.4 h | N/A |
E. faecalis | 2 | 35.0 h | 4.5 h | 30.5 | 0.1306‡ |
(23–47) | (2 – 7) | (21 – 40) | |||
Other Enterococci (includes E. faecium) | 2 | 92.5 h | 12.1 h | 80.h | 0.0522‡ |
(75 – 110) | (5 – 20) | (70 – 90) | |||
All Gram Negative | 2 | 101.5 h | 22.2 h | 79.3 h | 0.0204 |
K. pneumoniae | 2 | 101.5 h | 22.2 h | 79.3 h | 0.0204 |
(90 – 113) | (22.2) | (67.8 – 90.8) | |||