Extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa in camel in Egypt: potential human hazard

Background The rapid increase of extended-spectrum beta-lactamase (ESBL) producing bacteria are a potential health hazard. Development of antimicrobial resistance in animal pathogens has serious implications for human health, especially when such strains could be transmitted to human. In this study, the antimicrobial resistance due to ESBL producing Pseudomonas aeruginosa in the camel meat was investigated. Methods In this study meat samples from 200 healthy camels at two major abattoirs in Egypt (Cairo and Giza) were collected. Following culture on cetrimide agar, suspected P. aeruginosa colonies were confirmed with a Vitek 2 system (bioMe´rieux). P. aeruginosa isolates were phenotypically identified as ESBL by double disk synergy test. Additionally antimicrobial susceptibility testing of ESBL producing P. aeruginosa isolates were done against 11 antimicrobial drugs and carried out by disk diffusion method. The ESBL genotypes were determined by polymerase chain reaction according to the presence of the bla PER-1, bla CTX-M, bla SHV, and bla TEM. Results Pseudomonas aeruginosa was isolated from 45 camel meat sample (22.5%). The total percentage of ESBL producing P. aeruginosa was 45% (21/45) from camel meat isolates. Antibiogram results revealed the highest resistance was for c, ceftriaxone and rifampicin followed by cefepime and aztreonam. The prevalence rates of β-lactamase genes were recorded (bla PER-1 28.5%, bla CTX-M 38%, bla SHV 33.3% and bla TEM 23.8%). Conclusions This study illustrates the presence of high rates of ESBL-P. aeruginosa in camels that represents an increasing alarming for the risk of transmission to human and opens the door for current and future antibiotics therapy failure. Livestock associated ESBL-P. aeruginosa is a growing disaster, therefore, attention has to be fully given to livestock associated ESBL-bacteria which try to find its way to human beings.


Background
The increasing resistance of potentially pathogenic bacteria to multiple conventional antibiotics is an urgent problem in global public health [1]. Pseudomonas aeruginosa is one of the major causes of diseases such as otitis, mastitis, endometritis, hemorrhagic pneumonia and urinary tract infections in both livestock and companion animals [2][3][4]. The multiple-drug-resistant (MDR) Pseudomonas can be transmitted from different sources to humans and also to the environment through horizontal gene, the emergence and occurrence of MDR P. aeruginosa strains are growing in the world, leading to limited therapeutic options [5,6].
The prevalence of MDR enterobacteriaceae in slaughterhouses, including swine and poultry environments, has been reported in several studies building a growing alarm about their effect on animal and human health

Open Access
Annals of Clinical Microbiology and Antimicrobials [7,8]. Recently encountered the emergence of livestock associated ESBL-producing P. aeruginosa in cow, poultry and pigs [9,10]. In Middle East region, camels are important in the livestock economy by their adaptability to adverse environmental conditions and naturally resistant to most of the diseases commonly affecting livestock [11]. Camels provide milk, meat, wool, hides, and skin, and their dung is used for fires [12]. They are acting one of major and good sources of protein and income for developing countries.
Transmission of ESBL-producing gram-negative bacteria between food-producing animals and humans via direct contact or meat is supposed [13]. Accordingly, livestock associated ESBL-producing gram-negative bacteria become new alarm for emerging infectious pathogens to human and animals.
This study aimed to investigate the role of camels as a risky reservoir and disseminating carrier of ESBL producing P. aeruginosa especially as this micro-organism has the ability of producing multidrug resistant enzymes that could be easily disseminated in the community via livestock products, particularly through direct contact and/or consumption of meat as well as other infected animal products.

Camel meat samples
This study was carried out on 200 apparently healthy camels. These camels were selected from two major abattoirs (Cairo and Giza) from April to September 2015. Meat samples from slaughtered camels in the abattoirs were collected in sterile containers and sent on ice to the microbiology department laboratory.

Meat samples preparation
Twenty-five grams of the collected meat samples were weighed and transferred to sterile flasks containing 100 ml of phosphate buffer saline (PBS). Samples were homogenized using a meat grinder under aseptic conditions.

Isolation and identification of P. aeruginosa
Two hundred meat samples were cultured into cetrimide agar, the plates were incubated aerobically at 37 °C for 24 h. The putative colonies were examined for their colonial morphology and microscopically according to Quinn et al. [14], the purified isolates of P. aeruginosa were finally confirmed with a Vitek 2 system (bioMe´rieux) which is rapid and reliable identification method.

Phenotypic detection of ESBL by double-disk synergy test method (DDST)
ESBL production in P. aeruginosa was identified by the double disk synergy test (DDST) as described by Jarlier [15]. Mueller-Hinton agar was inoculated with standardized inoculum (corresponding to 0.5 McFarland tube) using a sterile cotton swab. An Augmentin (20 μg amoxicillin and 10 μg of clavulanic acid-AMC) disk was inserted in the center of the plate and test disks of 3rd generation cephalosporins (ceftazidime-CAZ 30 μg, ceftriaxone-CRO 30 μg, cefotaxime-CTX 30 μg) and aztreonam (ATM 30 μg) disks were placed at 20 mm distance (center to center) from the amoxicillin clavulanic acid disk. The plate was incubated overnight at 37 °C. Enhancement of the zone of inhibition of any one of the four drug disks toward amoxicillin-clavulanic acid suggested the presence of extended-spectrum beta-lactamases.
Escherichia coli 25922 was used as a negative control for the ESBL and P. aeruginosa ATCC 27853 was used as a control strain for a positive ESBL.

DNA extraction
The whole genomic DNA from ESBL resistant P. aeruginosa strains totaling 21 was extracted using QIAamp Mini Kit (QIAGEN, Hombrechtikon, Switzerland) according to the manufacturer's instructions.

Molecular detection of ESBL-encoding genes
PCRs for detection of the bla PER-1 , bla CTX-M , bla SHV and bla TEM genes. Specific primers for amplifying the selected genes by PCR are shown in Table 1.
The reaction mixture consisted of 25 µl Platinum ™ Hot Start PCR Master Mix (Invitrogen ™ ), 1 µl DNA extract, 0.5 µl of each primer in the concentration of (20 pmol) and nuclease free water up to 50 µl. The cycling conditions included denaturation for 10 min at 95 °C, amplification for 30 cycles of 30 s at 95 °C, 1 min at 55 °C, and 1 min at 72 °C, and extension for 10 min at 72 °C. The PCR products were resolved by electrophoresis on 1.5% (wt/vol) agarose gels (QIAGEN, Hombrechtikon, Switzerland).

Isolation and identification of P. aeruginosa
Forty-five isolates (22.5%) from meat samples of 200 camels were produced characteristic growth features of Pseudomonas species on the cetrimide agar medium. The isolates were obtained as one specific colony per camel meat sample. The isolates were confirmed as by Vitek 2 system (bioMe´rieux).

Phenotypic detection of ESBL by double-disk synergy test method (DDST)
Phenotypic detection of ESBL by DDST revealed that 21 P. aeruginosa isolates. Accordingly, a total percentage of ESBL was detected in overall, 45% (21/45) from camel meat isolates.

Molecular detection of ESBL-encoding genes
PCR screening of genes encoding ESBL revealed the amplification of bla PER-1 , bla CTX-M , bla SHV and bla TEM genes in all tested isolates except three isolates coded p-4, -11, and -18 did not harbor any of ESBL genes (Table 3).

Discussion
Extended-spectrum beta lactamase-producing bacteria are one of the fastest emerging resistance problems worldwide. ESBL-producing bacteria were observed in human medical practice, the observation of these bacteria in companion animals and the increase in livestock has initiated monitoring studies concentrating on livestock [13,21].
Livestock may be an important vehicle for the community-wide dissemination of antimicrobial-resistant Enterobacteriaceae, also P. aeruginosa especially ESBLproducing type isolates have been found in increasing numbers in food-producing animals [9,10,22].
Accordingly to the hypothesis that animals might become infection sources or even natural persistent sources acting as risky reservoirs of infection leading to the spread of these bacteria specifically multidrug resistant types in community [23]. There are essential needs for monitoring or surveillance studies incorporating veterinary medicine to identify transmissible pathogens to human and its risk factors.
The dromedary camel is a good source of meat, especially in areas where the climate adversely affects the performance of other meat animals. This is because of the unique physiological characteristics of camels [24]. The interest for camel meat seems by all accounts to be expanded because of wellbeing reasons, as it contains

Table 1 Primers used for detection of resistance genes
One uni plex PCR for detection of bla TEM gene and two multiplex PCR were conducted for the (bla PER-1 and bla CTX-M ) and another one for (bla SHV ) gene   16:21 less fat and in addition less cholesterol and generally high polyunsaturated unsaturated fats than other animal's meat [25].

Target gene Primer sequence (5′-3′) Product size (bp) References
To the best of our knowledge, this is the first time that an attempt has been made to determine ESBL P. aeruginosa resistance in camel meat. In this study, the prevalence of P. aeruginosa in camel was determined about 22.5% (45/200). This percent is so higher to the encountered investigations on fish and cow in Switzerland and Nigeria [9,26]. Even though P. aeruginosa is ubiquitous, the prevalence of its isolation among camel isolates in this study was lower compared to the presence rate of E. coli that isolated from camel in Saudi Arabia with 26.0% [27].
Class A (ESBLs) are typically identified in P. aeruginosa isolates and showing resistance to the extended-spectrum cephalosporin (ESCs) [28], this resistance is often due to the production of β-lactamases. Clinically, ESBLs are generally encoded by plasmid-mediated bla genes; three major clinically relevant β-lactamase genes are bla SHV , bla TEM and bla CTX-M 2, 4 [29].
CTX-M enzymes have now become the most widespread type of ESBL worldwide and represent the wide dissemination of particular plasmids or bacterial clones [30]. On the other hand, bla PER-1 were found to be the most prevalent type of β-lactamase-encoding genes in Acinetobacter baumannii in Egypt [31] and this suggests the horizontal transfer of this gene.
Pseudomonas aeruginosa and other gram negative bacteria with ESBLs contain other β-lactamases that makes difficult the phenotypic detection of ESBL [32], this issue need further investigation.
Many investigators focus on MDR P. aeruginosa from human. P. aeruginosa are multi-drug resistant to amikacin (17.25%), ciprofloxacin (27.59%), ceftriaxone varied from 51.00 to 73.00% and all the strains were susceptible to imipenem (20.69%) [33]. High resistance to aminoglycosides had been reported in Malaysia [34]. Similarly higher rates of resistance to fluoroquinolones such as ciprofloxacin (40.5%) had been reported in a study done in India. Lesser rate of resistance to ceftriaxone (40%) had been reported in Andhra Pradesh, India [35]. The presence of high resistance profile by camel P. aeruginosa isolates my attributed antibiotics used in management of this animals or natural resistance of camel that suites it as a risk reservoir for such pathogens.

Conclusion
The present study demonstrates, for the first time in Egypt, the presence a high rate of ESBL-P. aeruginosa in camels, The abundance MDR P. aeruginosa from camel meat in this study suggests a potential risk of human susceptibility to such pathogen group which remodels the epidemiology of antibiotic resistance. Urgent control measures are necessary to be applied to restrict the continuous abuse of antibiotics, especially in livestock production.