Annals of Clinical Microbiology and Antimicrobials Open Access Comprehensive Bactericidal Activity of an Ethanol-based Hand Gel in 15 Seconds

Background: Some studies indicate that the commonly recommended 30 s application time for the post contamination treatment of hands may not be necessary as the same effect may be achieved with some formulations in a shorter application time such as 15 s.


Introduction
The use of alcohol-based hand antiseptics has become a standard worldwide to prevent the transmission of nosocomial pathogens by the hands of the healthcare workers [1]. This development was enforced after the publication of the CDC guideline on hand hygiene [2] and will be even further enforced after publication of the upcoming WHO guideline on hand hygiene [3]. Most hand rubs are tested and recommended with an application time of 30 s [4][5][6]. Recent data, however, indicate that with some hand antiseptics the same efficacy may also be found in shorter application times such as 15 s when the hand antiseptic is applied correctly [7].
It has been recommended that a hand hygiene agent should have at least activity against bacteria, yeasts, and enveloped viruses [1]. A Propanol-based hand rub has been described before to have a broad spectrum of bactericidal activity within 30 s [8]. Some alcohol-based hand rubs have been described to easily inactivate clinically relevant enveloped viruses in only 15 s, even when the antiseptics were challenged with various types of organic load [9]. There may be the potential to reduce the recommended application time for the post contamination treatment of hands with well formulated hand antiseptics. In that respect it is essential to know if the clinically relevant pathogens are killed under the stringent test conditions of 15 s. We have therefore studied the bactericidal activity of an ethanol-based hand gel with an exposure time of only 15 s.

Hand rub
A hand gel based on 85% (w/w) ethanol was used for all experiments (Sterillium Comfort Gel, Bode Chemie GmbH & Co. KG, Hamburg, Germany). It was used because many other alcohol-based hand gels with a lower concentration of ethanol or propanol such as 70% or less were described to be significantly less effective than the European reference treatment and are not recommended for use in hospitals due to the limited efficacy [4]. All isolates were received from various hospital sources. The susceptibility of the isolates was tested using the Kirby-Bauer disk diffusion methods, CSLI (formerly NCCLS) interpretive standards were applied [10].

Test procedure
Inocula were prepared by transfer of bacteria from stock cultures into the appropriate broth media which were incubated for 18 -24 h at 37 ± 2°C. Haemophilus influenzae and Streptococcus pyogenes were incubated in the presence of 5% carbon dioxide, Bacteriodes fragilis and Clostridium difficile were incubated under anaerobic conditions. BBL GasPak ® Jar Systems were used to produce the anaerobic conditions. The GasPak system generates an anaerobic environment by means of a carbon dioxide and hydrogen generator, water, and a palladium catalyst. For Clostridium difficile only the vegetative cell form was investigated because it is known for more than 100 years that ethanol has no or little activity against bacterial spores [11][12][13][14][15] which is also supported by clinical data [16,17]. The bacterial test suspension served as the control (no exposure) and the pre-value.
Ninety-nine ml of the hand antiseptic were dispensed into four sterile flasks containing stir bars as described before [8]. Flasks were allowed to equilibrate to ambient room temperature for at least 10 min. One ml of bacterial test suspension was added to each flask. After the exposure time of 15 s an aliquot of 1 ml was transferred into tubes containing 9 ml Dey/Engley (D/E) neutralizing broth. Serial ten-fold dilutions were performed in phosphate buffered dilution water. Duplicate aliquots from selected dilutions were plated using the appropriate agar and plating technique. Nutrient agar pour plates were used for TSA containing 5% defibrinated rabbit's blood plates using both spread and filter plating technique were used for Listeria monocytogenes, ATCC 7644. For membrane filtration, the filter was applied aseptically with a forceps onto the stainless steel support. The disposable funnel was snapped into place on the filtration system. The membrane filter was wet with sterile diluent (0.85% saline).
The dilution tube was thoroughly mixed prior to sampling. The sample from the dilution tube was placed onto the filter surface, diluent was added and vacuum applied, using a vacuum pump and collection flask set up. The filter was removed aseptically from the filter holder with flamed forceps and place onto the surface of an agar plate [18]. Brain heart infusion agar pour plates were used for Acinetobacter lwoffi, Enterococcus faecium and Listeria monocytogenes (clinical isolate strain). Reinforced clostridial medium pour plates were used for Bacteroides fragilis and Clostridium difficile. Plates were incubated for the appropriate time and temperature as follows: Haemophilus influenzae for 36 to 50 h at 37 ± 2°C in the presence of 5% carbon dioxide, Bacteriodes fragilis and Clostridium difficile for 60 to 74 h at 37 ± 2°C under anaerobic conditions, and all remaining bacteria for 36 to 50 h at 37 ± 2°C in ambient air. After incubation colonies were counted and the number of colony-forming units (CFU) per ml calculated and transferred into a log 10 value.

Statistical analysis
Each experiment was carried out in quadruplicate. The log 10 reduction factor (RF) was calculated as the difference of the number of CFU per ml before and after exposure to the hand rub using the following formula: RF = log 10 cfu (control) -log 10 cfu (hand gel) The lowest RF of the four experiments with each strain is presented.

Results
The ethanol-based hand gel reduced all 11 Gram-positive and all 16 Gram-negative bacteria within 15 s by more than 5 log 10 -steps (Tables 1 and 2), not only against the ATCC test strains but also against corresponding clinical isolates. In addition, a RF > 5 was observed against all tested emerging bacterial pathogens ( Table 3). The lowest RF was always beyond the limit of detection.

Discussion
It has been suggested that a hand rub should have at least activity against bacteria, yeasts and enveloped viruses [1]. In our study only the bactericidal activity of a hand gel based on 85% (w/w) ethanol was looked at. We were able to show for the first time that the tested gel kills the most relevant nosocomial bacterial pathogens in only 15 s. Ethanol is known to have a strong bactericidal activity with log 10 reductions > 5 which has been demonstrated against some of the most common nosocomial pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa [7,[19][20][21][22] and which includes activity against various mycobacteria [23][24][25]. Nevertheless, this type of comprehensive bactericidal activity as described in our study has so far only been demonstrated with a propanol-based hand rub and an exposure time of 30 s [8].
Suspension tests, however, are not the critical part in the assessment of the efficacy assessment of alcohol-based hand antiseptics [26]. In a study with tests under practical conditions for hygienic hand disinfection (EN 1500) significant differences were observed between various alcohol-based preparations with quite poor results for those containing only up to 70% alcohol [4]. Recent data confirm that the efficacy of some alcohol-based hand antiseptics may be fairly low under practical test conditions (TFM test) especially when the overall concentration of alcohols is below 70% in the gel [27]. Suspension tests, however, can be considered to be less sensitive but are nevertheless very important to determine a general spectrum of antimicrobial activity.
We found that the gel kills the vegetative cell form of Clostridium difficile within 15 s which has been described before with a propanol-based hand rub in 30 s [8]. But with the emergence of Clostridium difficile in Europe and North America [28,29] it is important to understand that alcohols like ethanol or propanol have little or no activity against bacterial spores [11][12][13][14][15] which were not investigated in the present study. Therefore, it would be wrong to conclude that the application of alcohol-based hand rubs is sufficient when contamination of hands with Clostridium difficile is expected because both the vegetative cell form and the bacterial spore must be expected on hands next to each other. Promoting the use of alcohol-based hand rubs has been very effective to reduce the number of nosocomial infections by various bacterial species such as MRSA or VRE [16] but did not reduce or increase the  number of Clostridium difficile cases in hospitals [16,17]. Clostridium difficile, however, is never the single nosocomial pathogen on healthcare workers hands. Boyce et al. showed that fecal samples of patients with Clostridium difficile contain also in 9.8% MRSA [30]. That is why the best hand hygiene procedure seems to be to initially perform a hand disinfection in order to kill all clinically relevant bacteria including the vegetative cell form of Clostridium difficile. Immediately thereafter a thorough 10 s hand wash with plain soap should be done in order to reduce the number of spores on hands as much as possible [15]. A longer hand wash or use of an antimicrobial soap do not yield a better reduction of bacterial spores on hands [15] but can substantially damage the skin [31]. Performing only a hand wash without a hand disinfection does not take into account the vast majority of nosocomial bacterial pathogens which will still be there when Clostridium difficile outbreaks occur.
The main argument for a shorter application time in hand disinfection without any reduction of efficacy is certainly that it is easier to comply with [32]. The overall required time will be shorter [33] which can have a positive effect on the unknowingly attitude of healthcare workers towards performing a hand disinfection procedure. A high compliance rate in hand hygiene is a key issue for a successful prevention of nosocomial infections [34]. A shorter application time such as 15 s may well make it easier for the healthcare worker to comply with the recommended standard. At the same time a shorter application time such as 15 s may result in an incomplete coverage of the hand with the antiseptic agent as suggested recently [35]. But at the same time we have to realize that there is also no evidence to show that a 30 s application time generally ensures a complete coverage of hands. If a shorter application time results in a higher compliance rate in hand hygiene, it is likely to significantly reduce the rate of nosocomial infections which will be welcomed by all professionals in infection control. On the other side it is unknown if a twinkle-toed technique with some untreated skin areas foils the expectable effect on the nosocomial infection rate at all. May be the leaks are mostly at parts of the skin which are not relevant for the transmission of pathogens. But may be the leaks are mostly at skin sites which abolish the effect of the hand disinfection procedure all together. As long as this issue can not be solved scientifically, it remains unclear if a shorter application time such as 15 s provides more benefits or risks.

Conclusion
In summary, a hand gel based on 85% ethanol demonstrated a comprehensive bactericidal activity within 15 s. Future research will hopefully help to find out if a shorter application time for the post contamination treatment of hands provides more benefits or more risks.