Table 1 Tabular illustration of the used primers sequence, annealing temperatures (Ta), expected product sizes (bp), and their references
Gene name | Primer name | Primer sequence (5′-3′) | Annealing temperature Ta (ºC) | Product size (bp) | Reference |
|---|---|---|---|---|---|
mecA | mecA-F | AAAATCGATGGTAAAGGTTGGC | 53 | 533 | [47] |
mecA- R | AGTTCTGGAGTACCGGATTTGC | ||||
icaA | icaA-F | GAC CTC GAA GTC AAT AGA GGT | 60 | 814 | [45] |
icaA-R | CCC AGT ATA ACG TTG GAT ACC | ||||
Domain V of 23S rRNA | Domain V of 23S rRNA-F | GCGGTCGCCTCCTAAAAG | 55 | 390 | [9] |
Domain V of 23S rRNA-R | ATCCCGGTCCTCTCGTACTA | ||||
rplC | rplC-F | AACCTGATTTAGTTCCGTCTA | 822 | ||
rplc-R | GTTGACGCTTTAATGGGCTTA | ||||
rplD | rplD-F | TCGCTTACCTCCTTAATG | 1200 | ||
rplD-R | GGTGGAAACACTGTAACTG | ||||
rplV | rplV-F | CAACACGAAGTCCGATTGGA’ | 350 | ||
rplV-R | GCAGACGACAAGAAAACAAG | ||||
optrA | optrA-F | TACTTGATGAACCTACTAACCA | 422 | ||
optrA-R | CCTTGAACTACTGATTCTCGG | ||||
cfr | cfr-F | TGAAGTATAAAGCAGGTTGGGAGTC | 746 | ||
cfr-R | ACCATATAATTGACCACAAGCAGC | ||||
cfr(B) | cfr(B)-F | TGAGCATATACGAGTAACCTCAAGA’ | 58 | 293 | |
cfr(B)-R | CGCAAGCAGCGTCTATAT CA | ||||
vanA | vanA-F | ATG AAT AGA ATA AAA GTT GC | 50 | 1032 | [43] |
vanA-R | TCA CCC CTT TAA CGC TAA TA | ||||
msrA | msrA-F | GGC ACA ATA AGA GTG TTT AAA GG | 40 | 939 | [44] |
msrA-R | AAG TTA TAT CAT GAA TAG ATT GTC CTG TT |