Fig. 2

Separation and identification of (1,3)-β-D-glucans from Candida albicans. A Silver nitrate stainingand, and B Periodic acid-schiff staining: The (1 → 3) -β-D-glucan from Candida albicans was treated and analysed using Silver nitrate staining and periodic acid-schiff staining respectively; lane 1: The extraction of rude (1 → 3) -β-D-glucan from Candida albicans with Chemical method and ultrasonic crushing; lane 2:Deionized water blank control; lane 3 to lane 6: The rude glucans was digested with (1 → 3) -β-D-glucan in different times,1 h, 1.5 h, 2 h and 2.5 h respectively. C The low polymerization degree of (1 → 3) -β-D-glucan was purified by molecular sieve and stained by PAS; Dotted box indecated low molecular weight (1 → 3) -β-D-glucan as target in; D The interaction between aptamer and glucan was evaluated by western blot. Lane 1: 1 mg /ml; lane 2: 3 mg /ml; lane 3: glucose monomer, as control; E and F The purified low polymerization degree of (1 → 3) -β-D-glucan was anlysed by HPLC and elemental analysis. Arrow indicated the retention time of targeted product in HPLC system