Table 3 Mutation analysis of BDQ resistant genes among 205 MDR isolates
Resistance pattern | Isolate number | Gene mutation type | No. of isolates (%) | MIC of BDQ (μg/ml) | |||
|---|---|---|---|---|---|---|---|
atpE | Rv0678 | pepQ | Rv1979 | ||||
BDQ resistant isolates (9) | 22A050, 22A133, 22A148 | WT | CAG152CGG Gln51Arg | WT | WT | 3 | 0.500 |
22A118 | WT | TTC56TCC Phe19Ser | WT | WT | 1 | 0.500 | |
22A177 | WT | 492 position ins_GA | WT | WT | 1 | 1.000 | |
22A180 | WT | 274 position ins -A | WT | WT | 1 | 1.000 | |
Total | 6 (66.7)a | 0.500–1.000 | |||||
BDQ sensitive isolates (196) | 22A076 | WT | GGC307AGC Gly103Ser | WT | WT | 1 | 0.125 |
22A079 | WT | GCC184ACC Ala62Thr | WT | WT | 1 | 0.250 | |
22A128 | WT | WT | GCC411GCT Ala137Ala | WT | 1 | 0.031 | |
22A174 | WT | WT | GAA1080GAT/Glu360Asp | WT | 1 | 0.016 | |
22A012, 22A025, 22A030, 22A032, 22A041 | WT | WT | WT | GTT1276ATT/Val426lle | 5 | 0.031–0.063 | |
22A222 | WT | WT | WT | C(−70)G | 1 | 0.063 | |
22A196 | WT | WT | WT | GCG717GCA/Ala239Ala | 1 | 0.063 | |
22A005 | WT | WT | WT | TCG785TTG/Ser262Leu | 1 | 0.016 | |
22A227 | WT | WT | WT | GTC286ATC/Val96lle | 1 | 0.031 | |
22A204 | WT | WT | WT | GCC449GTC/Ala150Val | 1 | 0.250 | |
22A039 | WT | WT | WT | GTT155GGT/Val52Gly | 1 | 0.031 | |
22A016, 22A029, 22A201, 22A220, 22A223 | WT | WT | WT | WT | 5 | ≤ 0.008–0.031 | |
22A006, 22A009 | WT | WT | WT | WT | 2 | 0.031 | |
22A044 | WT | WT | WT | WT | 1 | 0.016 | |
22A206 | WT | WT | WT | WT | 1 | 0.031 | |
22A028 | WT | WT | WT | WT | 1 | ≤ 0.008 | |
22A008 | WT | WT | WT | WT | 1 | 0.031 | |
Total | 26 (13.3)b | ≤ 0.008–0.250 | |||||