Isolation, characterization, therapeutic potency, and genomic analysis of a novel bacteriophage vB_KshKPC-M against carbapenemase-producing Klebsiella pneumoniae strains (CRKP) isolated from Ventilator-associated pneumoniae (VAP) infection of COVID-19 patients

Mohammadi, Mehrdad; Saffari, Mahmood; Siadat, Seyed Davar; Hejazi, Seyed Hossein; Shayestehpour, Mohammad; Motallebi, Mitra; Eidi, Milad

doi:10.1186/s12941-023-00567-1

Annals of Clinical Microbiology and Antimicrobials

Table 2 Characteristics of clinical Klebsiella pneumoniae isolates

From: Isolation, characterization, therapeutic potency, and genomic analysis of a novel bacteriophage vB_KshKPC-M against carbapenemase-producing Klebsiella pneumoniae strains (CRKP) isolated from Ventilator-associated pneumoniae (VAP) infection of COVID-19 patients

Number	K.p isolated codes	The Resistance pattern of antibiotics	Klebsiella pneumoniae carbapenemase (KPC) isolates	Carba NP test	mCIM test	BlaKPC gene	BlaNDM gene	blaOXA-23 gene	Colistin MIC interpretation	Biofilm formation potency
1	Kp1	AMC, SXT, CTX, TOB, AZT, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
2	Kp2	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	−	−	Sensitive	Weakly
3	Kp3	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	+	−	Sensitive	Negative
4	Kp4	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	+	−	Sensitive	Negative
5	Kp5	Non-resistance	−	N	N	−	−	−	Not applicable	Negative
6	Kp6	AMC, SXT, CPM, CTX, TOB, AZT, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
7	Kp7	SXT, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
8	Kp8	AMC, SXT, CPM, CTX, TOB, AZT, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
9	Kp9	AMC, SXT, CPM, CTX, TOB, AZT, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
10	Kp10	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
11	Kp11	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	N	N	+	−	−	Sensitive	Moderate
12	Kp12	AMC, SXT, CPM, CTX, TOB, AZT, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
13	Kp13	AMC, CPM, AZT, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
14	Kp14	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	−	−	Sensitive	Negative
15	Kp15	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	N	+	−	−	Sensitive	Weakly
16	Kp16	SXT, CIP	−	N	N	−	−	−	Not applicable	Negative
17	Kp17	Non-resistance	−	N	N	−	−	−	Not applicable	Negative
18	Kp18	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Negative
19	Kp19	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Negative
20	Kp20	Non-resistance	−	N	N	−	−	−	Not applicable	Negative
21	Kp21	AMC, SXT, CIP, GM	−	N	N	−	−	−	Not applicable	Negative
22	Kp22	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Moderate
23	Kp23	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	+	Sensitive	Moderate
24	Kp24	SXT, CIP	−	N	N	−	−	−	Not applicable	Negative
25	Kp25	Non-resistance	−	N	N	−	−	−	Not applicable	Negative
26	Kp26	AMC, SXT, CIP, GM	−	N	N	−	−	−	Not applicable	Negative
27	Kp27	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	+	−	Sensitive	Negative
28	Kp28	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
29	Kp29	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Negative
30	Kp30	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Negative
31	Kp31	SXT, TOB, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
32	Kp32	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	−	−	Sensitive	Negative
33	Kp33	SXT, CIP	−	N	N	−	−	−	Not applicable	Negative
34	Kp34	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
35	Kp35	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Strong
36	Kp36	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	N	+	+	−	Sensitive	Moderate
37	Kp37	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	N	N	+	+	−	Sensitive	Negative
38	Kp38	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Moderate
39	Kp39	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Moderate
40	Kp40	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Weakly
41	Kp41	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	-	Sensitive	Moderate
42	Kp42	SXT, CIP	−	N	N	−	−	−	Not applicable	Negative
43	Kp43	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	+	−	Sensitive	Negative
44	Kp44	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	−	+	−	Sensitive	Moderate
45	Kp45	SXT, TOB, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
46	Kp46	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	−	−	Sensitive	Moderate
47	Kp47	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
48	Kp48	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
49	Kp49	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	+	−	Sensitive	Negative
50	Kp50	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	N	+	−	−	Sensitive	Negative
51	Kp51	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Negative
52	Kp52	AMC, SXT, CIP, GM	−	N	N	−	−	−	Not applicable	Negative
53	Kp53	SXT, TOB, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
54	Kp54	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	P	+	+	−	Sensitive	Negative
55	Kp55	SXT, TOB, CIP	−	N	N	−	−	−	Not applicable	Negative
56	Kp56	CPM, TOB, AZT, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
57	Kp57	MER, TOB, CIP	−	N	N	−	−	−	Not applicable	Negative
58	Kp58	MER, CPM, TOB, AZT, IMI, GM, CIP, PTZ	+	P	P	+	+	−	Sensitive	Negative
59	Kp59	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
60	Kp60	SXT, TOB, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
61	Kp61	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	-	Not applicable	Negative
62	Kp62	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	−	−	Sensitive	Negative
63	Kp63	SXT, TOB, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
64	Kp64	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Strong
65	Kp65	SXT, CPM, AZT, GM, CIP, CTX, PT	−	N	N	−	−	−	Not applicable	Weak
66	Kp66	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
67	Kp67	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
68	Kp68	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
69	Kp69	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	+	−	Sensitive	Negative
70	Kp70	SXT, TOB, CIP, PTZ	−	N	P	−	−	−	Not applicable	Negative
71	Kp71	AMC, SXT, CIP, GM	−	N	N	−	−	−	Not applicable	Negative
72	Kp72	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
73	Kp73	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	−	−	Sensitive	Negative
74	Kp74	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	−	−	Sensitive	Negative
75	Kp75	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	+	−	Sensitive	Negative
76	Kp76	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Negative
77	Kp77	AMC, SXT, MER, CPM, TOB, AZT, IMI, CIP, GM, CTX, PTZ	+	P	N	+	−	−	Sensitive	Moderate
78	Kp78	AMC, SXT, CIP, GM	−	N	N	−	−	−	Not applicable	Negative
79	Kp79	Non-resistance	−	N	N	−	−	−	Not applicable	Negative
80	Kp80	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	−	−	Sensitive	Weakly
81	Kp81	AMC, SXT, CIP, GM	−	N	N	−	−	−	Not applicable	Negative
82	Kp82	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	−	−	Sensitive	Weakly
83	Kp83	SXT, PTZ, CIP	−	N	N	−	−	−	Not applicable	Negative
84	Kp84	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
85	Kp85	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	+	−	Sensitive	Moderate
86	Kp86	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
87	Kp87	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	−	−	Not applicable	Negative
88	Kp88	SXT, PTZ, CIP	−	N	N	−	−	−	Not applicable	Negative
89	Kp89	SXT, CPM, AZT, GM, CIP, CTX, PTZ	−	N	N	−	−	−	Not applicable	Negative
90	Kp90	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	−	−	Sensitive	Moderate
91	Kp91	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Moderate
92	Kp92	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	+	−	Not applicable	Negative
93	Kp93	SXT, PTZ, CIP	−	N	N	−	−	−	Not applicable	Negative
94	Kp94	SXT, MER, CPM, CTX, AZT, IMI, ETP, GM, CIP, PTZ	+	P	P	+	−	−	Sensitive	Negative
95	Kp95	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Sensitive	Strong
96	Kp96	SXT, CPM, TOB, AZT, CTX, GM, CIP, PTZ	−	N	N	−	+	−	Not applicable	Negative
97	Kp97	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PT	+	P	P	+	+	+	Sensitive	Strong
98	Kp98	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Resistance	Moderate
99	Kp99	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	−	Resistance	Moderate
100	Kp100	AMC, SXT, MER, CPM, TOB, AZT, IMI, GEM, ETP, CIP, CTX, PTZ	+	P	P	+	+	+	Resistance	Strong

Trimethoprim/sulfamethoxazole (1.25/23.75 μg) (SXT), Amoxicillin-clavulanate (20/10 μg) (AMC), piperacillin/tazobactam (100/10 μg) (PTZ), Cefepime (30 μg) (CPM), Cefotaxime (30 μg) (CTX), Ciprofloxacin (5 μg) (CIP), Imipenem (10 μg) (IMI), Ertapenem (10 μg) (ETP), Meropenem (10 μg) (MER), Tobramycin (10 μg) (TOB), Gentamicin (10 μg) (GM), Aztreonam (30 μg) (AZT). Kp: Klebsiella pneumoniae, P positive, N Negative

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ISSN: 1476-0711

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